A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs

BACKGROUND: Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management. RESULTS: Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B....

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Detalles Bibliográficos
Autores principales: Liu, I-Li, Chi, Nai-Yu, Chang, Chia-Ling, Hung, Ming-Long, Chiu, Chun-Ta, Chen, Hui-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884907/
https://www.ncbi.nlm.nih.gov/pubmed/31783850
http://dx.doi.org/10.1186/s12917-019-2181-5
Descripción
Sumario:BACKGROUND: Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management. RESULTS: Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B. gibsoni near the dog patient. Within 30 min, this diagnostic assay was able to detect as low as 0.002% parasitemia of the dog blood. Using clinical samples, this new assay was validated to demonstrate 100% agreement with real-time PCR. CONCLUSIONS: This novel diagnostic method provides a reliable point-of-care test to assist in the identification of B. gibsoni.

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