A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs

BACKGROUND: Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management. RESULTS: Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B....

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Autores principales: Liu, I-Li, Chi, Nai-Yu, Chang, Chia-Ling, Hung, Ming-Long, Chiu, Chun-Ta, Chen, Hui-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884907/
https://www.ncbi.nlm.nih.gov/pubmed/31783850
http://dx.doi.org/10.1186/s12917-019-2181-5
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author Liu, I-Li
Chi, Nai-Yu
Chang, Chia-Ling
Hung, Ming-Long
Chiu, Chun-Ta
Chen, Hui-Wen
author_facet Liu, I-Li
Chi, Nai-Yu
Chang, Chia-Ling
Hung, Ming-Long
Chiu, Chun-Ta
Chen, Hui-Wen
author_sort Liu, I-Li
collection PubMed
description BACKGROUND: Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management. RESULTS: Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B. gibsoni near the dog patient. Within 30 min, this diagnostic assay was able to detect as low as 0.002% parasitemia of the dog blood. Using clinical samples, this new assay was validated to demonstrate 100% agreement with real-time PCR. CONCLUSIONS: This novel diagnostic method provides a reliable point-of-care test to assist in the identification of B. gibsoni.
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spelling pubmed-68849072019-12-03 A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs Liu, I-Li Chi, Nai-Yu Chang, Chia-Ling Hung, Ming-Long Chiu, Chun-Ta Chen, Hui-Wen BMC Vet Res Methodology Article BACKGROUND: Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management. RESULTS: Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B. gibsoni near the dog patient. Within 30 min, this diagnostic assay was able to detect as low as 0.002% parasitemia of the dog blood. Using clinical samples, this new assay was validated to demonstrate 100% agreement with real-time PCR. CONCLUSIONS: This novel diagnostic method provides a reliable point-of-care test to assist in the identification of B. gibsoni. BioMed Central 2019-11-29 /pmc/articles/PMC6884907/ /pubmed/31783850 http://dx.doi.org/10.1186/s12917-019-2181-5 Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology Article
Liu, I-Li
Chi, Nai-Yu
Chang, Chia-Ling
Hung, Ming-Long
Chiu, Chun-Ta
Chen, Hui-Wen
A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs
title A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs
title_full A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs
title_fullStr A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs
title_full_unstemmed A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs
title_short A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs
title_sort novel pcr-based point-of-care method enables rapid, sensitive and reliable diagnosis of babesia gibsoni infection in dogs
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884907/
https://www.ncbi.nlm.nih.gov/pubmed/31783850
http://dx.doi.org/10.1186/s12917-019-2181-5
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