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Alteration of Extracellular Matrix Molecules and Perineuronal Nets in the Hippocampus of Pentylenetetrazol-Kindled Mice

The pathophysiological processes leading to epilepsy are poorly understood. Understanding the molecular and cellular mechanisms involved in the onset of epilepsy is crucial for drug development. Epileptogenicity is thought to be associated with changes in synaptic plasticity; however, whether extrac...

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Autores principales: Ueno, Hiroshi, Suemitsu, Shunsuke, Murakami, Shinji, Kitamura, Naoya, Wani, Kenta, Takahashi, Yu, Matsumoto, Yosuke, Okamoto, Motoi, Ishihara, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885262/
https://www.ncbi.nlm.nih.gov/pubmed/31827499
http://dx.doi.org/10.1155/2019/8924634
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author Ueno, Hiroshi
Suemitsu, Shunsuke
Murakami, Shinji
Kitamura, Naoya
Wani, Kenta
Takahashi, Yu
Matsumoto, Yosuke
Okamoto, Motoi
Ishihara, Takeshi
author_facet Ueno, Hiroshi
Suemitsu, Shunsuke
Murakami, Shinji
Kitamura, Naoya
Wani, Kenta
Takahashi, Yu
Matsumoto, Yosuke
Okamoto, Motoi
Ishihara, Takeshi
author_sort Ueno, Hiroshi
collection PubMed
description The pathophysiological processes leading to epilepsy are poorly understood. Understanding the molecular and cellular mechanisms involved in the onset of epilepsy is crucial for drug development. Epileptogenicity is thought to be associated with changes in synaptic plasticity; however, whether extracellular matrix molecules—known regulators of synaptic plasticity—are altered during epileptogenesis is unknown. To test this, we used a pentylenetetrazole- (PTZ-) kindling model mouse to investigate changes to hippocampal parvalbumin- (PV-) positive neurons, extracellular matrix molecules, and perineuronal nets (PNNs) after the last kindled seizure. We found an increase in Wisteria floribunda agglutinin- (WFA-) and Cat-315-positive PNNs and a decrease in PV-positive neurons not surrounded by PNNs, in the hippocampus of PTZ-kindled mice compared to control mice. Furthermore, the expression of WFA- and Cat-315-positive molecules increased in the extracellular space of PTZ-kindled mice. In addition, consistent with previous studies, astrocytes were activated in PTZ-kindled mice. We propose that the increase in PNNs after kindling decreases neuroplasticity in the hippocampus and helps maintain the neural circuit for recurrent seizures. This study shows that possibility of changes in extracellular matrix molecules due to astrocyte activation is associated with epilepticus in PTZ-kindled mice.
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spelling pubmed-68852622019-12-11 Alteration of Extracellular Matrix Molecules and Perineuronal Nets in the Hippocampus of Pentylenetetrazol-Kindled Mice Ueno, Hiroshi Suemitsu, Shunsuke Murakami, Shinji Kitamura, Naoya Wani, Kenta Takahashi, Yu Matsumoto, Yosuke Okamoto, Motoi Ishihara, Takeshi Neural Plast Research Article The pathophysiological processes leading to epilepsy are poorly understood. Understanding the molecular and cellular mechanisms involved in the onset of epilepsy is crucial for drug development. Epileptogenicity is thought to be associated with changes in synaptic plasticity; however, whether extracellular matrix molecules—known regulators of synaptic plasticity—are altered during epileptogenesis is unknown. To test this, we used a pentylenetetrazole- (PTZ-) kindling model mouse to investigate changes to hippocampal parvalbumin- (PV-) positive neurons, extracellular matrix molecules, and perineuronal nets (PNNs) after the last kindled seizure. We found an increase in Wisteria floribunda agglutinin- (WFA-) and Cat-315-positive PNNs and a decrease in PV-positive neurons not surrounded by PNNs, in the hippocampus of PTZ-kindled mice compared to control mice. Furthermore, the expression of WFA- and Cat-315-positive molecules increased in the extracellular space of PTZ-kindled mice. In addition, consistent with previous studies, astrocytes were activated in PTZ-kindled mice. We propose that the increase in PNNs after kindling decreases neuroplasticity in the hippocampus and helps maintain the neural circuit for recurrent seizures. This study shows that possibility of changes in extracellular matrix molecules due to astrocyte activation is associated with epilepticus in PTZ-kindled mice. Hindawi 2019-11-14 /pmc/articles/PMC6885262/ /pubmed/31827499 http://dx.doi.org/10.1155/2019/8924634 Text en Copyright © 2019 Hiroshi Ueno et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ueno, Hiroshi
Suemitsu, Shunsuke
Murakami, Shinji
Kitamura, Naoya
Wani, Kenta
Takahashi, Yu
Matsumoto, Yosuke
Okamoto, Motoi
Ishihara, Takeshi
Alteration of Extracellular Matrix Molecules and Perineuronal Nets in the Hippocampus of Pentylenetetrazol-Kindled Mice
title Alteration of Extracellular Matrix Molecules and Perineuronal Nets in the Hippocampus of Pentylenetetrazol-Kindled Mice
title_full Alteration of Extracellular Matrix Molecules and Perineuronal Nets in the Hippocampus of Pentylenetetrazol-Kindled Mice
title_fullStr Alteration of Extracellular Matrix Molecules and Perineuronal Nets in the Hippocampus of Pentylenetetrazol-Kindled Mice
title_full_unstemmed Alteration of Extracellular Matrix Molecules and Perineuronal Nets in the Hippocampus of Pentylenetetrazol-Kindled Mice
title_short Alteration of Extracellular Matrix Molecules and Perineuronal Nets in the Hippocampus of Pentylenetetrazol-Kindled Mice
title_sort alteration of extracellular matrix molecules and perineuronal nets in the hippocampus of pentylenetetrazol-kindled mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885262/
https://www.ncbi.nlm.nih.gov/pubmed/31827499
http://dx.doi.org/10.1155/2019/8924634
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