Non-templated addition and template switching by Moloney murine leukemia virus (MMLV)-based reverse transcriptases co-occur and compete with each other

Single-cell RNA-Seq (scRNA-Seq) has led to an unprecedented understanding of gene expression and regulation in individual cells. Many scRNA-Seq approaches rely upon the template switching property of Moloney murine leukemia virus (MMLV)-type reverse transcriptases. Template switching is believed to...

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Detalles Bibliográficos
Autores principales: Wulf, Madalee G., Maguire, Sean, Humbert, Paul, Dai, Nan, Bei, Yanxia, Nichols, Nicole M., Corrêa, Ivan R., Guan, Shengxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2019
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885630/
https://www.ncbi.nlm.nih.gov/pubmed/31640989
http://dx.doi.org/10.1074/jbc.RA119.010676

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