Cargando…

Application of volumetric absorptive microsampling (VAMS) to measure multidimensional anti-influenza IgG antibodies by the mPlex-Flu assay

Introduction: Recently, volumetric absorptive microsampling (VAMS) has been used for accurate sampling of a fixed peripheral blood volume (10 µL) on a volumetric swab, and long-term sample storage. The mPlex-Flu assay is a novel, high-throughput assay that simultaneously measures the concentration o...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jiong, Li, Dongmei, Wiltse, Alexander, Emo, Jason, Hilchey, Shannon P., Zand, Martin S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885997/
https://www.ncbi.nlm.nih.gov/pubmed/31827907
http://dx.doi.org/10.1017/cts.2019.410
_version_ 1783474816521601024
author Wang, Jiong
Li, Dongmei
Wiltse, Alexander
Emo, Jason
Hilchey, Shannon P.
Zand, Martin S.
author_facet Wang, Jiong
Li, Dongmei
Wiltse, Alexander
Emo, Jason
Hilchey, Shannon P.
Zand, Martin S.
author_sort Wang, Jiong
collection PubMed
description Introduction: Recently, volumetric absorptive microsampling (VAMS) has been used for accurate sampling of a fixed peripheral blood volume (10 µL) on a volumetric swab, and long-term sample storage. The mPlex-Flu assay is a novel, high-throughput assay that simultaneously measures the concentration of antibodies against the hemagglutinin (HA) proteins from multiple influenza virus strains with ≤5 µL of serum. Here we describe combining these two methods to measure multidimensional anti-influenza IgG activity in whole blood samples collected by a finger stick and VAMS, with correction for serum volume based on simultaneous hemoglobin measurement. Methods: We compared capillary blood samples obtained from a finger stick using a VAMS device with serum samples collected by traditional phlebotomy from 20 subjects, with the influenza antibody profiles measured by the mPlex-Flu assay. Results: We found that results with the two sampling methods were highly correlated within subjects and across all influenza strains (mean R(2) = 0.9470). Adjustment for serum volume, based on hemaglobin measurement, was used to estimate serum volume of samples and improved the accuracy. IgG measurements were stable over 3 weeks when VAMS samples were stored at room temperature or transported using a variety of shipping methods. Additionally, when volunteers performed finger-stick VAMS at-home by themselves, the comparison results of anti-HA antibody concentrations were highly consistent with sampling performed by study personnel on-site (R(2) = 0.9496). Conclusions: This novel approach can provide a simple, accurate, and low-cost means for monitoring the IgG anti-influenza HA antibody responses in large population studies and clinical trials.
format Online
Article
Text
id pubmed-6885997
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Cambridge University Press
record_format MEDLINE/PubMed
spelling pubmed-68859972019-12-11 Application of volumetric absorptive microsampling (VAMS) to measure multidimensional anti-influenza IgG antibodies by the mPlex-Flu assay Wang, Jiong Li, Dongmei Wiltse, Alexander Emo, Jason Hilchey, Shannon P. Zand, Martin S. J Clin Transl Sci Research Article Introduction: Recently, volumetric absorptive microsampling (VAMS) has been used for accurate sampling of a fixed peripheral blood volume (10 µL) on a volumetric swab, and long-term sample storage. The mPlex-Flu assay is a novel, high-throughput assay that simultaneously measures the concentration of antibodies against the hemagglutinin (HA) proteins from multiple influenza virus strains with ≤5 µL of serum. Here we describe combining these two methods to measure multidimensional anti-influenza IgG activity in whole blood samples collected by a finger stick and VAMS, with correction for serum volume based on simultaneous hemoglobin measurement. Methods: We compared capillary blood samples obtained from a finger stick using a VAMS device with serum samples collected by traditional phlebotomy from 20 subjects, with the influenza antibody profiles measured by the mPlex-Flu assay. Results: We found that results with the two sampling methods were highly correlated within subjects and across all influenza strains (mean R(2) = 0.9470). Adjustment for serum volume, based on hemaglobin measurement, was used to estimate serum volume of samples and improved the accuracy. IgG measurements were stable over 3 weeks when VAMS samples were stored at room temperature or transported using a variety of shipping methods. Additionally, when volunteers performed finger-stick VAMS at-home by themselves, the comparison results of anti-HA antibody concentrations were highly consistent with sampling performed by study personnel on-site (R(2) = 0.9496). Conclusions: This novel approach can provide a simple, accurate, and low-cost means for monitoring the IgG anti-influenza HA antibody responses in large population studies and clinical trials. Cambridge University Press 2019-09-26 /pmc/articles/PMC6885997/ /pubmed/31827907 http://dx.doi.org/10.1017/cts.2019.410 Text en © The Association for Clinical and Translational Science 2019 http://creativecommons.org/licenses/by/4.0/ This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Jiong
Li, Dongmei
Wiltse, Alexander
Emo, Jason
Hilchey, Shannon P.
Zand, Martin S.
Application of volumetric absorptive microsampling (VAMS) to measure multidimensional anti-influenza IgG antibodies by the mPlex-Flu assay
title Application of volumetric absorptive microsampling (VAMS) to measure multidimensional anti-influenza IgG antibodies by the mPlex-Flu assay
title_full Application of volumetric absorptive microsampling (VAMS) to measure multidimensional anti-influenza IgG antibodies by the mPlex-Flu assay
title_fullStr Application of volumetric absorptive microsampling (VAMS) to measure multidimensional anti-influenza IgG antibodies by the mPlex-Flu assay
title_full_unstemmed Application of volumetric absorptive microsampling (VAMS) to measure multidimensional anti-influenza IgG antibodies by the mPlex-Flu assay
title_short Application of volumetric absorptive microsampling (VAMS) to measure multidimensional anti-influenza IgG antibodies by the mPlex-Flu assay
title_sort application of volumetric absorptive microsampling (vams) to measure multidimensional anti-influenza igg antibodies by the mplex-flu assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885997/
https://www.ncbi.nlm.nih.gov/pubmed/31827907
http://dx.doi.org/10.1017/cts.2019.410
work_keys_str_mv AT wangjiong applicationofvolumetricabsorptivemicrosamplingvamstomeasuremultidimensionalantiinfluenzaiggantibodiesbythemplexfluassay
AT lidongmei applicationofvolumetricabsorptivemicrosamplingvamstomeasuremultidimensionalantiinfluenzaiggantibodiesbythemplexfluassay
AT wiltsealexander applicationofvolumetricabsorptivemicrosamplingvamstomeasuremultidimensionalantiinfluenzaiggantibodiesbythemplexfluassay
AT emojason applicationofvolumetricabsorptivemicrosamplingvamstomeasuremultidimensionalantiinfluenzaiggantibodiesbythemplexfluassay
AT hilcheyshannonp applicationofvolumetricabsorptivemicrosamplingvamstomeasuremultidimensionalantiinfluenzaiggantibodiesbythemplexfluassay
AT zandmartins applicationofvolumetricabsorptivemicrosamplingvamstomeasuremultidimensionalantiinfluenzaiggantibodiesbythemplexfluassay