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Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections
BACKGROUND & OBJECTIVES: Respiratory syncytial virus (RSV) and Mycoplasma pneumoniae are considered common cause of lower respiratory tract infections (LRTIs) in children. The present study was conducted to detect M. pneumoniae and RSV in paediatric LRTIs employing serology, polymerase chain rea...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6886145/ https://www.ncbi.nlm.nih.gov/pubmed/31719302 http://dx.doi.org/10.4103/ijmr.IJMR_983_18 |
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author | Kumar, Sanchit Chakravarti, Anita Kumar, Surinder Kapoor, Seema |
author_facet | Kumar, Sanchit Chakravarti, Anita Kumar, Surinder Kapoor, Seema |
author_sort | Kumar, Sanchit |
collection | PubMed |
description | BACKGROUND & OBJECTIVES: Respiratory syncytial virus (RSV) and Mycoplasma pneumoniae are considered common cause of lower respiratory tract infections (LRTIs) in children. The present study was conducted to detect M. pneumoniae and RSV in paediatric LRTIs employing serology, polymerase chain reaction (PCR) and reverse transcriptase PCR (RT-PCR) analysis. METHODS: Seventy five children aged one month to five years with acute LRTIs were investigated for M. pneumoniae antibodies and RSV antigen using immunochromatographic test, RT-PCR for RSV and M. pneumoniae by PCR on nasopharyngeal aspirates. RESULTS: RSV infection was observed in 33 (44%) and M. pneumoniae was positive in 26 (35%) children. No significant difference in infection was noted between male and female children. Clinical and radiological features among RSV and M. pneumoniae positive and negative cases were similar. Considering RT-PCR for RSV as gold standard, RSV antigen immunochromatography was 90.90 per cent sensitive and 100 per cent specific. INTERPRETATION & CONCLUSIONS: Our study showed the presence of RSV and M. pneumoniae infection in 44 and 35 per cent children, respectively with community-acquired LRTIs and aged less than five years. |
format | Online Article Text |
id | pubmed-6886145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-68861452019-12-09 Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections Kumar, Sanchit Chakravarti, Anita Kumar, Surinder Kapoor, Seema Indian J Med Res Student IJMR BACKGROUND & OBJECTIVES: Respiratory syncytial virus (RSV) and Mycoplasma pneumoniae are considered common cause of lower respiratory tract infections (LRTIs) in children. The present study was conducted to detect M. pneumoniae and RSV in paediatric LRTIs employing serology, polymerase chain reaction (PCR) and reverse transcriptase PCR (RT-PCR) analysis. METHODS: Seventy five children aged one month to five years with acute LRTIs were investigated for M. pneumoniae antibodies and RSV antigen using immunochromatographic test, RT-PCR for RSV and M. pneumoniae by PCR on nasopharyngeal aspirates. RESULTS: RSV infection was observed in 33 (44%) and M. pneumoniae was positive in 26 (35%) children. No significant difference in infection was noted between male and female children. Clinical and radiological features among RSV and M. pneumoniae positive and negative cases were similar. Considering RT-PCR for RSV as gold standard, RSV antigen immunochromatography was 90.90 per cent sensitive and 100 per cent specific. INTERPRETATION & CONCLUSIONS: Our study showed the presence of RSV and M. pneumoniae infection in 44 and 35 per cent children, respectively with community-acquired LRTIs and aged less than five years. Wolters Kluwer - Medknow 2019-09 /pmc/articles/PMC6886145/ /pubmed/31719302 http://dx.doi.org/10.4103/ijmr.IJMR_983_18 Text en Copyright: © 2019 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Student IJMR Kumar, Sanchit Chakravarti, Anita Kumar, Surinder Kapoor, Seema Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections |
title | Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections |
title_full | Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections |
title_fullStr | Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections |
title_full_unstemmed | Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections |
title_short | Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections |
title_sort | detection of respiratory syncytial virus & mycoplasma pneumoniae in paediatric lower respiratory tract infections |
topic | Student IJMR |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6886145/ https://www.ncbi.nlm.nih.gov/pubmed/31719302 http://dx.doi.org/10.4103/ijmr.IJMR_983_18 |
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