Cargando…

Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections

BACKGROUND & OBJECTIVES: Respiratory syncytial virus (RSV) and Mycoplasma pneumoniae are considered common cause of lower respiratory tract infections (LRTIs) in children. The present study was conducted to detect M. pneumoniae and RSV in paediatric LRTIs employing serology, polymerase chain rea...

Descripción completa

Detalles Bibliográficos
Autores principales: Kumar, Sanchit, Chakravarti, Anita, Kumar, Surinder, Kapoor, Seema
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6886145/
https://www.ncbi.nlm.nih.gov/pubmed/31719302
http://dx.doi.org/10.4103/ijmr.IJMR_983_18
_version_ 1783474826317398016
author Kumar, Sanchit
Chakravarti, Anita
Kumar, Surinder
Kapoor, Seema
author_facet Kumar, Sanchit
Chakravarti, Anita
Kumar, Surinder
Kapoor, Seema
author_sort Kumar, Sanchit
collection PubMed
description BACKGROUND & OBJECTIVES: Respiratory syncytial virus (RSV) and Mycoplasma pneumoniae are considered common cause of lower respiratory tract infections (LRTIs) in children. The present study was conducted to detect M. pneumoniae and RSV in paediatric LRTIs employing serology, polymerase chain reaction (PCR) and reverse transcriptase PCR (RT-PCR) analysis. METHODS: Seventy five children aged one month to five years with acute LRTIs were investigated for M. pneumoniae antibodies and RSV antigen using immunochromatographic test, RT-PCR for RSV and M. pneumoniae by PCR on nasopharyngeal aspirates. RESULTS: RSV infection was observed in 33 (44%) and M. pneumoniae was positive in 26 (35%) children. No significant difference in infection was noted between male and female children. Clinical and radiological features among RSV and M. pneumoniae positive and negative cases were similar. Considering RT-PCR for RSV as gold standard, RSV antigen immunochromatography was 90.90 per cent sensitive and 100 per cent specific. INTERPRETATION & CONCLUSIONS: Our study showed the presence of RSV and M. pneumoniae infection in 44 and 35 per cent children, respectively with community-acquired LRTIs and aged less than five years.
format Online
Article
Text
id pubmed-6886145
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Wolters Kluwer - Medknow
record_format MEDLINE/PubMed
spelling pubmed-68861452019-12-09 Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections Kumar, Sanchit Chakravarti, Anita Kumar, Surinder Kapoor, Seema Indian J Med Res Student IJMR BACKGROUND & OBJECTIVES: Respiratory syncytial virus (RSV) and Mycoplasma pneumoniae are considered common cause of lower respiratory tract infections (LRTIs) in children. The present study was conducted to detect M. pneumoniae and RSV in paediatric LRTIs employing serology, polymerase chain reaction (PCR) and reverse transcriptase PCR (RT-PCR) analysis. METHODS: Seventy five children aged one month to five years with acute LRTIs were investigated for M. pneumoniae antibodies and RSV antigen using immunochromatographic test, RT-PCR for RSV and M. pneumoniae by PCR on nasopharyngeal aspirates. RESULTS: RSV infection was observed in 33 (44%) and M. pneumoniae was positive in 26 (35%) children. No significant difference in infection was noted between male and female children. Clinical and radiological features among RSV and M. pneumoniae positive and negative cases were similar. Considering RT-PCR for RSV as gold standard, RSV antigen immunochromatography was 90.90 per cent sensitive and 100 per cent specific. INTERPRETATION & CONCLUSIONS: Our study showed the presence of RSV and M. pneumoniae infection in 44 and 35 per cent children, respectively with community-acquired LRTIs and aged less than five years. Wolters Kluwer - Medknow 2019-09 /pmc/articles/PMC6886145/ /pubmed/31719302 http://dx.doi.org/10.4103/ijmr.IJMR_983_18 Text en Copyright: © 2019 Indian Journal of Medical Research http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Student IJMR
Kumar, Sanchit
Chakravarti, Anita
Kumar, Surinder
Kapoor, Seema
Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections
title Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections
title_full Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections
title_fullStr Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections
title_full_unstemmed Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections
title_short Detection of respiratory syncytial virus & Mycoplasma pneumoniae in paediatric lower respiratory tract infections
title_sort detection of respiratory syncytial virus & mycoplasma pneumoniae in paediatric lower respiratory tract infections
topic Student IJMR
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6886145/
https://www.ncbi.nlm.nih.gov/pubmed/31719302
http://dx.doi.org/10.4103/ijmr.IJMR_983_18
work_keys_str_mv AT kumarsanchit detectionofrespiratorysyncytialvirusmycoplasmapneumoniaeinpaediatriclowerrespiratorytractinfections
AT chakravartianita detectionofrespiratorysyncytialvirusmycoplasmapneumoniaeinpaediatriclowerrespiratorytractinfections
AT kumarsurinder detectionofrespiratorysyncytialvirusmycoplasmapneumoniaeinpaediatriclowerrespiratorytractinfections
AT kapoorseema detectionofrespiratorysyncytialvirusmycoplasmapneumoniaeinpaediatriclowerrespiratorytractinfections