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Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit
After tooth extraction, the alveolar bone tends to shrink in volume, especially on the vestibular side. The role of myofibroblasts in bone remodeling has not been sufficiently investigated. The aim of the present study was to explore the gene expression related to myofibroblasts presence and activit...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888118/ https://www.ncbi.nlm.nih.gov/pubmed/31717520 http://dx.doi.org/10.3390/ma12223697 |
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author | Marconcini, Simone Denaro, Maria Cosola, Saverio Gabriele, Mario Toti, Paolo Mijiritsky, Eitan Proietti, Agnese Basolo, Fulvio Giammarinaro, Enrica Covani, Ugo |
author_facet | Marconcini, Simone Denaro, Maria Cosola, Saverio Gabriele, Mario Toti, Paolo Mijiritsky, Eitan Proietti, Agnese Basolo, Fulvio Giammarinaro, Enrica Covani, Ugo |
author_sort | Marconcini, Simone |
collection | PubMed |
description | After tooth extraction, the alveolar bone tends to shrink in volume, especially on the vestibular side. The role of myofibroblasts in bone remodeling has not been sufficiently investigated. The aim of the present study was to explore the gene expression related to myofibroblasts presence and activity during a 90-day healing period after tooth extraction. The study included 36 rabbits, and a single tooth extraction was performed on each rabbit. The extractive sockets were randomly distributed to natural healing or to scarification of the wound. The sacrifices were staggered in such a manner that animals contributed with sockets representing 2, 7, 15, 30, 60, and 90 days of healing. Nanostring technology was used to evaluate the expression of a wide panel consisting in 148 genes related to the activation, induction, and suppression of myofibroblasts, socket microenvironment, and autophagy. We found that the expression profile of this custom panel was time-related. The post-extractive socket was subjected to significant gene expression changes after 15 days: the genes involved in the induction of myofibroblasts were up-regulated in the first 15-day period and down-regulated during the rest of the follow-up. The study suggested that myofibroblasts play a major role in the immediate 15-day period following tooth extraction. |
format | Online Article Text |
id | pubmed-6888118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68881182019-12-09 Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit Marconcini, Simone Denaro, Maria Cosola, Saverio Gabriele, Mario Toti, Paolo Mijiritsky, Eitan Proietti, Agnese Basolo, Fulvio Giammarinaro, Enrica Covani, Ugo Materials (Basel) Article After tooth extraction, the alveolar bone tends to shrink in volume, especially on the vestibular side. The role of myofibroblasts in bone remodeling has not been sufficiently investigated. The aim of the present study was to explore the gene expression related to myofibroblasts presence and activity during a 90-day healing period after tooth extraction. The study included 36 rabbits, and a single tooth extraction was performed on each rabbit. The extractive sockets were randomly distributed to natural healing or to scarification of the wound. The sacrifices were staggered in such a manner that animals contributed with sockets representing 2, 7, 15, 30, 60, and 90 days of healing. Nanostring technology was used to evaluate the expression of a wide panel consisting in 148 genes related to the activation, induction, and suppression of myofibroblasts, socket microenvironment, and autophagy. We found that the expression profile of this custom panel was time-related. The post-extractive socket was subjected to significant gene expression changes after 15 days: the genes involved in the induction of myofibroblasts were up-regulated in the first 15-day period and down-regulated during the rest of the follow-up. The study suggested that myofibroblasts play a major role in the immediate 15-day period following tooth extraction. MDPI 2019-11-09 /pmc/articles/PMC6888118/ /pubmed/31717520 http://dx.doi.org/10.3390/ma12223697 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marconcini, Simone Denaro, Maria Cosola, Saverio Gabriele, Mario Toti, Paolo Mijiritsky, Eitan Proietti, Agnese Basolo, Fulvio Giammarinaro, Enrica Covani, Ugo Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit |
title | Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit |
title_full | Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit |
title_fullStr | Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit |
title_full_unstemmed | Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit |
title_short | Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit |
title_sort | myofibroblast gene expression profile after tooth extraction in the rabbit |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888118/ https://www.ncbi.nlm.nih.gov/pubmed/31717520 http://dx.doi.org/10.3390/ma12223697 |
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