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Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit

After tooth extraction, the alveolar bone tends to shrink in volume, especially on the vestibular side. The role of myofibroblasts in bone remodeling has not been sufficiently investigated. The aim of the present study was to explore the gene expression related to myofibroblasts presence and activit...

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Autores principales: Marconcini, Simone, Denaro, Maria, Cosola, Saverio, Gabriele, Mario, Toti, Paolo, Mijiritsky, Eitan, Proietti, Agnese, Basolo, Fulvio, Giammarinaro, Enrica, Covani, Ugo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888118/
https://www.ncbi.nlm.nih.gov/pubmed/31717520
http://dx.doi.org/10.3390/ma12223697
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author Marconcini, Simone
Denaro, Maria
Cosola, Saverio
Gabriele, Mario
Toti, Paolo
Mijiritsky, Eitan
Proietti, Agnese
Basolo, Fulvio
Giammarinaro, Enrica
Covani, Ugo
author_facet Marconcini, Simone
Denaro, Maria
Cosola, Saverio
Gabriele, Mario
Toti, Paolo
Mijiritsky, Eitan
Proietti, Agnese
Basolo, Fulvio
Giammarinaro, Enrica
Covani, Ugo
author_sort Marconcini, Simone
collection PubMed
description After tooth extraction, the alveolar bone tends to shrink in volume, especially on the vestibular side. The role of myofibroblasts in bone remodeling has not been sufficiently investigated. The aim of the present study was to explore the gene expression related to myofibroblasts presence and activity during a 90-day healing period after tooth extraction. The study included 36 rabbits, and a single tooth extraction was performed on each rabbit. The extractive sockets were randomly distributed to natural healing or to scarification of the wound. The sacrifices were staggered in such a manner that animals contributed with sockets representing 2, 7, 15, 30, 60, and 90 days of healing. Nanostring technology was used to evaluate the expression of a wide panel consisting in 148 genes related to the activation, induction, and suppression of myofibroblasts, socket microenvironment, and autophagy. We found that the expression profile of this custom panel was time-related. The post-extractive socket was subjected to significant gene expression changes after 15 days: the genes involved in the induction of myofibroblasts were up-regulated in the first 15-day period and down-regulated during the rest of the follow-up. The study suggested that myofibroblasts play a major role in the immediate 15-day period following tooth extraction.
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spelling pubmed-68881182019-12-09 Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit Marconcini, Simone Denaro, Maria Cosola, Saverio Gabriele, Mario Toti, Paolo Mijiritsky, Eitan Proietti, Agnese Basolo, Fulvio Giammarinaro, Enrica Covani, Ugo Materials (Basel) Article After tooth extraction, the alveolar bone tends to shrink in volume, especially on the vestibular side. The role of myofibroblasts in bone remodeling has not been sufficiently investigated. The aim of the present study was to explore the gene expression related to myofibroblasts presence and activity during a 90-day healing period after tooth extraction. The study included 36 rabbits, and a single tooth extraction was performed on each rabbit. The extractive sockets were randomly distributed to natural healing or to scarification of the wound. The sacrifices were staggered in such a manner that animals contributed with sockets representing 2, 7, 15, 30, 60, and 90 days of healing. Nanostring technology was used to evaluate the expression of a wide panel consisting in 148 genes related to the activation, induction, and suppression of myofibroblasts, socket microenvironment, and autophagy. We found that the expression profile of this custom panel was time-related. The post-extractive socket was subjected to significant gene expression changes after 15 days: the genes involved in the induction of myofibroblasts were up-regulated in the first 15-day period and down-regulated during the rest of the follow-up. The study suggested that myofibroblasts play a major role in the immediate 15-day period following tooth extraction. MDPI 2019-11-09 /pmc/articles/PMC6888118/ /pubmed/31717520 http://dx.doi.org/10.3390/ma12223697 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marconcini, Simone
Denaro, Maria
Cosola, Saverio
Gabriele, Mario
Toti, Paolo
Mijiritsky, Eitan
Proietti, Agnese
Basolo, Fulvio
Giammarinaro, Enrica
Covani, Ugo
Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit
title Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit
title_full Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit
title_fullStr Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit
title_full_unstemmed Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit
title_short Myofibroblast Gene Expression Profile after Tooth Extraction in the Rabbit
title_sort myofibroblast gene expression profile after tooth extraction in the rabbit
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888118/
https://www.ncbi.nlm.nih.gov/pubmed/31717520
http://dx.doi.org/10.3390/ma12223697
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