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Provitamin A Biofortification of Durum Wheat through a TILLING Approach

Macro- and micronutrients, essential for the maintenance of human metabolism, are assimilated daily through the diet. Wheat and other major cereals are a good source of nutrients, such as carbohydrates and proteins, but cannot supply a sufficient amount of essential micronutrients, including provita...

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Autores principales: Sestili, Francesco, Garcia-Molina, Maria Dolores, Gambacorta, Gianluca, Beleggia, Romina, Botticella, Ermelinda, De Vita, Pasquale, Savatin, Daniel Valentin, Masci, Stefania, Lafiandra, Domenico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888361/
https://www.ncbi.nlm.nih.gov/pubmed/31739436
http://dx.doi.org/10.3390/ijms20225703
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author Sestili, Francesco
Garcia-Molina, Maria Dolores
Gambacorta, Gianluca
Beleggia, Romina
Botticella, Ermelinda
De Vita, Pasquale
Savatin, Daniel Valentin
Masci, Stefania
Lafiandra, Domenico
author_facet Sestili, Francesco
Garcia-Molina, Maria Dolores
Gambacorta, Gianluca
Beleggia, Romina
Botticella, Ermelinda
De Vita, Pasquale
Savatin, Daniel Valentin
Masci, Stefania
Lafiandra, Domenico
author_sort Sestili, Francesco
collection PubMed
description Macro- and micronutrients, essential for the maintenance of human metabolism, are assimilated daily through the diet. Wheat and other major cereals are a good source of nutrients, such as carbohydrates and proteins, but cannot supply a sufficient amount of essential micronutrients, including provitamin A. As vitamin A deficiency (VAD) leads to several serious diseases throughout the world, the biofortification of a major staple crop, such as wheat, represents an effective way to preserve human health in developing countries. In the present work, a key enzyme involved in the branch of carotenoids pathway producing β-carotene, lycopene epsilon cyclase, has been targeted by a Targeting Induced Local Lesions in Genomes (TILLING) approach in a “block strategy” perspective. The null mutant genotype showed a strong reduction in the expression of the lcyE gene and also interesting pleiotropic effects on an enzyme (β-ring hydroxylase) acting downstream in the pathway. Biochemical profiling of carotenoids in the wheat mutant lines showed an increase of roughly 75% in β-carotene in the grains of the complete mutant line compared with the control. In conclusion, we describe here the production and characterization of a new wheat line biofortified with provitamin A obtained through a nontransgenic approach, which also sheds new light on the molecular mechanism governing carotenoid biosynthesis in durum wheat.
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spelling pubmed-68883612019-12-09 Provitamin A Biofortification of Durum Wheat through a TILLING Approach Sestili, Francesco Garcia-Molina, Maria Dolores Gambacorta, Gianluca Beleggia, Romina Botticella, Ermelinda De Vita, Pasquale Savatin, Daniel Valentin Masci, Stefania Lafiandra, Domenico Int J Mol Sci Article Macro- and micronutrients, essential for the maintenance of human metabolism, are assimilated daily through the diet. Wheat and other major cereals are a good source of nutrients, such as carbohydrates and proteins, but cannot supply a sufficient amount of essential micronutrients, including provitamin A. As vitamin A deficiency (VAD) leads to several serious diseases throughout the world, the biofortification of a major staple crop, such as wheat, represents an effective way to preserve human health in developing countries. In the present work, a key enzyme involved in the branch of carotenoids pathway producing β-carotene, lycopene epsilon cyclase, has been targeted by a Targeting Induced Local Lesions in Genomes (TILLING) approach in a “block strategy” perspective. The null mutant genotype showed a strong reduction in the expression of the lcyE gene and also interesting pleiotropic effects on an enzyme (β-ring hydroxylase) acting downstream in the pathway. Biochemical profiling of carotenoids in the wheat mutant lines showed an increase of roughly 75% in β-carotene in the grains of the complete mutant line compared with the control. In conclusion, we describe here the production and characterization of a new wheat line biofortified with provitamin A obtained through a nontransgenic approach, which also sheds new light on the molecular mechanism governing carotenoid biosynthesis in durum wheat. MDPI 2019-11-14 /pmc/articles/PMC6888361/ /pubmed/31739436 http://dx.doi.org/10.3390/ijms20225703 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sestili, Francesco
Garcia-Molina, Maria Dolores
Gambacorta, Gianluca
Beleggia, Romina
Botticella, Ermelinda
De Vita, Pasquale
Savatin, Daniel Valentin
Masci, Stefania
Lafiandra, Domenico
Provitamin A Biofortification of Durum Wheat through a TILLING Approach
title Provitamin A Biofortification of Durum Wheat through a TILLING Approach
title_full Provitamin A Biofortification of Durum Wheat through a TILLING Approach
title_fullStr Provitamin A Biofortification of Durum Wheat through a TILLING Approach
title_full_unstemmed Provitamin A Biofortification of Durum Wheat through a TILLING Approach
title_short Provitamin A Biofortification of Durum Wheat through a TILLING Approach
title_sort provitamin a biofortification of durum wheat through a tilling approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888361/
https://www.ncbi.nlm.nih.gov/pubmed/31739436
http://dx.doi.org/10.3390/ijms20225703
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