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Curcumin Ameliorates Benzo[a]pyrene-Induced DNA Damages in Stomach Tissues of Sprague-Dawley Rats

Benzo[a]pyrene (BaP) is a well-known carcinogen formed during the cooking process. Although BaP exposure has been implicated as one of the risk factors for lung cancer in animals and humans, there are only limited data on BaP-induced gastrointestinal cancer. Therefore, this study investigated the pr...

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Autores principales: Kim, Kyeong Seok, Kim, Na Yoon, Son, Ji Yeon, Park, Jae Hyeon, Lee, Su Hyun, Kim, Hae Ri, Kim, Boomin, Kim, Yoon Gyoon, Jeong, Hye Gwang, Lee, Byung Mu, Kim, Hyung Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888507/
https://www.ncbi.nlm.nih.gov/pubmed/31698770
http://dx.doi.org/10.3390/ijms20225533
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author Kim, Kyeong Seok
Kim, Na Yoon
Son, Ji Yeon
Park, Jae Hyeon
Lee, Su Hyun
Kim, Hae Ri
Kim, Boomin
Kim, Yoon Gyoon
Jeong, Hye Gwang
Lee, Byung Mu
Kim, Hyung Sik
author_facet Kim, Kyeong Seok
Kim, Na Yoon
Son, Ji Yeon
Park, Jae Hyeon
Lee, Su Hyun
Kim, Hae Ri
Kim, Boomin
Kim, Yoon Gyoon
Jeong, Hye Gwang
Lee, Byung Mu
Kim, Hyung Sik
author_sort Kim, Kyeong Seok
collection PubMed
description Benzo[a]pyrene (BaP) is a well-known carcinogen formed during the cooking process. Although BaP exposure has been implicated as one of the risk factors for lung cancer in animals and humans, there are only limited data on BaP-induced gastrointestinal cancer. Therefore, this study investigated the protective effects of curcumin on BaP-induced DNA damage in rat stomach tissues. BaP (20 mg/kg/day) and curcumin (50, 100, or 200 mg/kg) were administered daily to Sprague-Dawley rats by oral gavage over 30 days. Curcumin was pre-administered before BaP exposure. All rats were euthanized, and liver, kidney, and stomach tissues were removed at 24 h after the last treatment. We observed that aspartate aminotransferase (AST), alanine aminotransferase (ALT), and glucose levels were significantly reduced in rats treated with high dose co-administration of curcumin (200 mg/kg) compared to BaP alone. The expression levels of cytochrome P450 (CYP) 1A1 and CYP1B1 were significantly increased in the liver of rats treated with BaP. However, co-administration of curcumin (200 mg/kg) with BaP markedly reduced CYP1A1 expression in a dose-dependent manner. Furthermore, plasma levels of BaP-diolepoxide (BPDE) and BaP metabolites were significantly reduced by co-administration of curcumin (200 mg/kg). Additionally, co-administration of curcumin (200 mg/kg) with BaP significantly reduced the formation of BPDE-I-DNA and 8-hydroxydeoxy guanosine (8-OHdG) adducts in the liver, kidney, and stomach tissues. The inhibition of these adduct formations were more prominent in the stomach tissues than in the liver. Overall, our observations suggest that curcumin might inhibit BaP-induced gastrointestinal tumorigenesis and shows promise as a chemopreventive agent.
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spelling pubmed-68885072019-12-09 Curcumin Ameliorates Benzo[a]pyrene-Induced DNA Damages in Stomach Tissues of Sprague-Dawley Rats Kim, Kyeong Seok Kim, Na Yoon Son, Ji Yeon Park, Jae Hyeon Lee, Su Hyun Kim, Hae Ri Kim, Boomin Kim, Yoon Gyoon Jeong, Hye Gwang Lee, Byung Mu Kim, Hyung Sik Int J Mol Sci Article Benzo[a]pyrene (BaP) is a well-known carcinogen formed during the cooking process. Although BaP exposure has been implicated as one of the risk factors for lung cancer in animals and humans, there are only limited data on BaP-induced gastrointestinal cancer. Therefore, this study investigated the protective effects of curcumin on BaP-induced DNA damage in rat stomach tissues. BaP (20 mg/kg/day) and curcumin (50, 100, or 200 mg/kg) were administered daily to Sprague-Dawley rats by oral gavage over 30 days. Curcumin was pre-administered before BaP exposure. All rats were euthanized, and liver, kidney, and stomach tissues were removed at 24 h after the last treatment. We observed that aspartate aminotransferase (AST), alanine aminotransferase (ALT), and glucose levels were significantly reduced in rats treated with high dose co-administration of curcumin (200 mg/kg) compared to BaP alone. The expression levels of cytochrome P450 (CYP) 1A1 and CYP1B1 were significantly increased in the liver of rats treated with BaP. However, co-administration of curcumin (200 mg/kg) with BaP markedly reduced CYP1A1 expression in a dose-dependent manner. Furthermore, plasma levels of BaP-diolepoxide (BPDE) and BaP metabolites were significantly reduced by co-administration of curcumin (200 mg/kg). Additionally, co-administration of curcumin (200 mg/kg) with BaP significantly reduced the formation of BPDE-I-DNA and 8-hydroxydeoxy guanosine (8-OHdG) adducts in the liver, kidney, and stomach tissues. The inhibition of these adduct formations were more prominent in the stomach tissues than in the liver. Overall, our observations suggest that curcumin might inhibit BaP-induced gastrointestinal tumorigenesis and shows promise as a chemopreventive agent. MDPI 2019-11-06 /pmc/articles/PMC6888507/ /pubmed/31698770 http://dx.doi.org/10.3390/ijms20225533 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Kyeong Seok
Kim, Na Yoon
Son, Ji Yeon
Park, Jae Hyeon
Lee, Su Hyun
Kim, Hae Ri
Kim, Boomin
Kim, Yoon Gyoon
Jeong, Hye Gwang
Lee, Byung Mu
Kim, Hyung Sik
Curcumin Ameliorates Benzo[a]pyrene-Induced DNA Damages in Stomach Tissues of Sprague-Dawley Rats
title Curcumin Ameliorates Benzo[a]pyrene-Induced DNA Damages in Stomach Tissues of Sprague-Dawley Rats
title_full Curcumin Ameliorates Benzo[a]pyrene-Induced DNA Damages in Stomach Tissues of Sprague-Dawley Rats
title_fullStr Curcumin Ameliorates Benzo[a]pyrene-Induced DNA Damages in Stomach Tissues of Sprague-Dawley Rats
title_full_unstemmed Curcumin Ameliorates Benzo[a]pyrene-Induced DNA Damages in Stomach Tissues of Sprague-Dawley Rats
title_short Curcumin Ameliorates Benzo[a]pyrene-Induced DNA Damages in Stomach Tissues of Sprague-Dawley Rats
title_sort curcumin ameliorates benzo[a]pyrene-induced dna damages in stomach tissues of sprague-dawley rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888507/
https://www.ncbi.nlm.nih.gov/pubmed/31698770
http://dx.doi.org/10.3390/ijms20225533
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