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A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose

Chronic vasopressin secretion induced by recurrent mild heat stress exposure is significantly enhanced by limited rehydration with a fructose-containing beverage both in rodents and in humans. Moreover, this effect has been associated with upregulation of the polyol–fructokinase pathway and increase...

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Autores principales: García-Arroyo, Fernando E., Muñoz-Jiménez, Itzel, Gonzaga, Guillermo, Tapia, Edilia, Osorio-Alonso, Horacio, Roncal-Jiménez, Carlos A, Iroz, Alison, Vecchio, Mariacristina, Reyes-García, Juan G., Johnson, Richard J, Sánchez-Lozada, L Gabriela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888540/
https://www.ncbi.nlm.nih.gov/pubmed/31744099
http://dx.doi.org/10.3390/ijms20225764
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author García-Arroyo, Fernando E.
Muñoz-Jiménez, Itzel
Gonzaga, Guillermo
Tapia, Edilia
Osorio-Alonso, Horacio
Roncal-Jiménez, Carlos A
Iroz, Alison
Vecchio, Mariacristina
Reyes-García, Juan G.
Johnson, Richard J
Sánchez-Lozada, L Gabriela
author_facet García-Arroyo, Fernando E.
Muñoz-Jiménez, Itzel
Gonzaga, Guillermo
Tapia, Edilia
Osorio-Alonso, Horacio
Roncal-Jiménez, Carlos A
Iroz, Alison
Vecchio, Mariacristina
Reyes-García, Juan G.
Johnson, Richard J
Sánchez-Lozada, L Gabriela
author_sort García-Arroyo, Fernando E.
collection PubMed
description Chronic vasopressin secretion induced by recurrent mild heat stress exposure is significantly enhanced by limited rehydration with a fructose-containing beverage both in rodents and in humans. Moreover, this effect has been associated with upregulation of the polyol–fructokinase pathway and increased renal oxidative stress. Previously, we have shown that pharmacological inhibition of both V1a and V2 vasopressin receptors with conivaptan improved such renal alterations. The aim of this study was to evaluate the independent contributions of V1a and V2 receptors to the renal damage caused by mild heat stress and limited rehydration with a fructose-containing beverage. Osmotic minipumps were used to deliver either relcovaptan (0.64 mg/day) or tolvaptan (0.25 mg/day) in male Wistar rats for two weeks. Corresponding dilution vehicles were used as controls. To induce dehydration, rats were exposed to mild heat stress (37 °C for 1 h, Monday to Friday). All groups received a 10% fructose solution as a rehydration fluid for 2 h after mild heat stress. For the remainder of the day and on weekends, rats received tap water. The independent blockade of either the V1a or the V2 receptor prevented renal damage, reduced oxidative stress, and decreased plasma cortisol and systemic inflammation. However, the beneficial effects were regulated by different mechanisms. Tolvaptan inhibited polyol–fructokinase pathway overactivation, while relcovaptan prevented upregulation of the renin–angiotensin system and SGK1 expression. These data suggest that both V1a and V2 receptors participate in renal damage caused by heat stress-induced dehydration when fructose-containing beverages are used as rehydration fluids.
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spelling pubmed-68885402019-12-09 A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose García-Arroyo, Fernando E. Muñoz-Jiménez, Itzel Gonzaga, Guillermo Tapia, Edilia Osorio-Alonso, Horacio Roncal-Jiménez, Carlos A Iroz, Alison Vecchio, Mariacristina Reyes-García, Juan G. Johnson, Richard J Sánchez-Lozada, L Gabriela Int J Mol Sci Article Chronic vasopressin secretion induced by recurrent mild heat stress exposure is significantly enhanced by limited rehydration with a fructose-containing beverage both in rodents and in humans. Moreover, this effect has been associated with upregulation of the polyol–fructokinase pathway and increased renal oxidative stress. Previously, we have shown that pharmacological inhibition of both V1a and V2 vasopressin receptors with conivaptan improved such renal alterations. The aim of this study was to evaluate the independent contributions of V1a and V2 receptors to the renal damage caused by mild heat stress and limited rehydration with a fructose-containing beverage. Osmotic minipumps were used to deliver either relcovaptan (0.64 mg/day) or tolvaptan (0.25 mg/day) in male Wistar rats for two weeks. Corresponding dilution vehicles were used as controls. To induce dehydration, rats were exposed to mild heat stress (37 °C for 1 h, Monday to Friday). All groups received a 10% fructose solution as a rehydration fluid for 2 h after mild heat stress. For the remainder of the day and on weekends, rats received tap water. The independent blockade of either the V1a or the V2 receptor prevented renal damage, reduced oxidative stress, and decreased plasma cortisol and systemic inflammation. However, the beneficial effects were regulated by different mechanisms. Tolvaptan inhibited polyol–fructokinase pathway overactivation, while relcovaptan prevented upregulation of the renin–angiotensin system and SGK1 expression. These data suggest that both V1a and V2 receptors participate in renal damage caused by heat stress-induced dehydration when fructose-containing beverages are used as rehydration fluids. MDPI 2019-11-16 /pmc/articles/PMC6888540/ /pubmed/31744099 http://dx.doi.org/10.3390/ijms20225764 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
García-Arroyo, Fernando E.
Muñoz-Jiménez, Itzel
Gonzaga, Guillermo
Tapia, Edilia
Osorio-Alonso, Horacio
Roncal-Jiménez, Carlos A
Iroz, Alison
Vecchio, Mariacristina
Reyes-García, Juan G.
Johnson, Richard J
Sánchez-Lozada, L Gabriela
A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose
title A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose
title_full A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose
title_fullStr A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose
title_full_unstemmed A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose
title_short A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose
title_sort role for both v1a and v2 receptors in renal heat stress injury amplified by rehydration with fructose
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888540/
https://www.ncbi.nlm.nih.gov/pubmed/31744099
http://dx.doi.org/10.3390/ijms20225764
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