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Tissue-Wide Gene Expression Analysis of Sodium/Phosphate Co-Transporters in Pigs

Sodium/phosphate co-transporters are considered to be important mediators of phosphorus (P) homeostasis. The expression of specific sodium/phosphate co-transporters is routinely used as an immediate response to dietary interventions in different species. However, a general understanding of their tis...

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Autores principales: Wubuli, Aisanjiang, Reyer, Henry, Muráni, Eduard, Ponsuksili, Siriluck, Wolf, Petra, Oster, Michael, Wimmers, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888643/
https://www.ncbi.nlm.nih.gov/pubmed/31717287
http://dx.doi.org/10.3390/ijms20225576
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author Wubuli, Aisanjiang
Reyer, Henry
Muráni, Eduard
Ponsuksili, Siriluck
Wolf, Petra
Oster, Michael
Wimmers, Klaus
author_facet Wubuli, Aisanjiang
Reyer, Henry
Muráni, Eduard
Ponsuksili, Siriluck
Wolf, Petra
Oster, Michael
Wimmers, Klaus
author_sort Wubuli, Aisanjiang
collection PubMed
description Sodium/phosphate co-transporters are considered to be important mediators of phosphorus (P) homeostasis. The expression of specific sodium/phosphate co-transporters is routinely used as an immediate response to dietary interventions in different species. However, a general understanding of their tissue-specificity is required to elucidate their particular contribution to P homeostasis. In this study, the tissue-wide gene expression status of all currently annotated sodium/phosphate co-transporters were investigated in two pig trials focusing on a standard commercial diet (trial 1) or divergent P-containing diets (trial 2). A wide range of tissues including the gastrointestinal tract (stomach, duodenum, jejunum, ileum, caecum, and colon), kidney, liver, bone, muscle, lung, and aorta were analyzed. Both trials showed consistent patterns in the overall tissue-specific expression of P transporters. While SLC34A2 was considered as the most important intestinal P transporter in other species including humans, SLC34A3 appeared to be the most prominent intestinal P transporter in pigs. In addition, the P transporters of the SLC17 family showed basal expression in the pig intestine and might have a contribution to P homeostasis. The expression patterns observed in the distal colon provide evidence that the large intestine may also be relevant for intestinal P absorption. A low dietary P supply induced higher expressions of SLC20A1, SLC20A2, SLC34A1, and SLC34A3 in the kidney cortex. The results suggest that the expression of genes encoding transcellular P transporters is tissue-specific and responsive to dietary P supply, while underlying regulatory mechanisms require further analyses.
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spelling pubmed-68886432019-12-09 Tissue-Wide Gene Expression Analysis of Sodium/Phosphate Co-Transporters in Pigs Wubuli, Aisanjiang Reyer, Henry Muráni, Eduard Ponsuksili, Siriluck Wolf, Petra Oster, Michael Wimmers, Klaus Int J Mol Sci Article Sodium/phosphate co-transporters are considered to be important mediators of phosphorus (P) homeostasis. The expression of specific sodium/phosphate co-transporters is routinely used as an immediate response to dietary interventions in different species. However, a general understanding of their tissue-specificity is required to elucidate their particular contribution to P homeostasis. In this study, the tissue-wide gene expression status of all currently annotated sodium/phosphate co-transporters were investigated in two pig trials focusing on a standard commercial diet (trial 1) or divergent P-containing diets (trial 2). A wide range of tissues including the gastrointestinal tract (stomach, duodenum, jejunum, ileum, caecum, and colon), kidney, liver, bone, muscle, lung, and aorta were analyzed. Both trials showed consistent patterns in the overall tissue-specific expression of P transporters. While SLC34A2 was considered as the most important intestinal P transporter in other species including humans, SLC34A3 appeared to be the most prominent intestinal P transporter in pigs. In addition, the P transporters of the SLC17 family showed basal expression in the pig intestine and might have a contribution to P homeostasis. The expression patterns observed in the distal colon provide evidence that the large intestine may also be relevant for intestinal P absorption. A low dietary P supply induced higher expressions of SLC20A1, SLC20A2, SLC34A1, and SLC34A3 in the kidney cortex. The results suggest that the expression of genes encoding transcellular P transporters is tissue-specific and responsive to dietary P supply, while underlying regulatory mechanisms require further analyses. MDPI 2019-11-08 /pmc/articles/PMC6888643/ /pubmed/31717287 http://dx.doi.org/10.3390/ijms20225576 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wubuli, Aisanjiang
Reyer, Henry
Muráni, Eduard
Ponsuksili, Siriluck
Wolf, Petra
Oster, Michael
Wimmers, Klaus
Tissue-Wide Gene Expression Analysis of Sodium/Phosphate Co-Transporters in Pigs
title Tissue-Wide Gene Expression Analysis of Sodium/Phosphate Co-Transporters in Pigs
title_full Tissue-Wide Gene Expression Analysis of Sodium/Phosphate Co-Transporters in Pigs
title_fullStr Tissue-Wide Gene Expression Analysis of Sodium/Phosphate Co-Transporters in Pigs
title_full_unstemmed Tissue-Wide Gene Expression Analysis of Sodium/Phosphate Co-Transporters in Pigs
title_short Tissue-Wide Gene Expression Analysis of Sodium/Phosphate Co-Transporters in Pigs
title_sort tissue-wide gene expression analysis of sodium/phosphate co-transporters in pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888643/
https://www.ncbi.nlm.nih.gov/pubmed/31717287
http://dx.doi.org/10.3390/ijms20225576
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