Cargando…

The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome

Chemokines are important in the development and progression of tumors. We investigated the expression of CXCL14 and CXCL16 in colon cancer. Expression of mRNA was assessed in primary tumors and lymph nodes and CXCL16 mRNA levels were correlated to patient’s survival. Protein expression was investiga...

Descripción completa

Detalles Bibliográficos
Autores principales: AbdelMageed, Manar, Ali, Haytham, Olsson, Lina, Lindmark, Gudrun, Hammarström, Marie-Louise, Hammarström, Sten, Sitohy, Basel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888697/
https://www.ncbi.nlm.nih.gov/pubmed/31752131
http://dx.doi.org/10.3390/ijms20225793
_version_ 1783475290274529280
author AbdelMageed, Manar
Ali, Haytham
Olsson, Lina
Lindmark, Gudrun
Hammarström, Marie-Louise
Hammarström, Sten
Sitohy, Basel
author_facet AbdelMageed, Manar
Ali, Haytham
Olsson, Lina
Lindmark, Gudrun
Hammarström, Marie-Louise
Hammarström, Sten
Sitohy, Basel
author_sort AbdelMageed, Manar
collection PubMed
description Chemokines are important in the development and progression of tumors. We investigated the expression of CXCL14 and CXCL16 in colon cancer. Expression of mRNA was assessed in primary tumors and lymph nodes and CXCL16 mRNA levels were correlated to patient’s survival. Protein expression was investigated by two-color immunofluorescence and immunomorphometry. CXCL14 and CXCL16 mRNA levels and protein expression were significantly higher in colon cancer primary tumors compared to apparently normal colon tissue. Positive cells were tumor cells, as revealed by anti-CEA and anti-EpCAM staining. CXCL16, but not CXCL14, mRNA levels were significantly higher in hematoxylin and eosin positive (H&E(+)) compared to H&E(−) colon cancer lymph nodes or control nodes (P < 0.0001). CXCL16 mRNA was expressed in 5/5 colon cancer cell lines while CXCL14 was expressed significantly in only one. Kaplan-Meier analysis revealed that colon cancer patients with lymph nodes expressing high or very high levels (7.2 and 11.4 copies/18S rRNA unit, respectively) of CXCL16 mRNA had a decreased mean survival time of 30 and 46 months at the 12-year follow-up (P = 0.04, P = 0.005, respectively). In conclusion, high expression of CXCL16 mRNA in regional lymph nodes of colon cancer patients is a sign of a poor prognosis.
format Online
Article
Text
id pubmed-6888697
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68886972019-12-09 The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome AbdelMageed, Manar Ali, Haytham Olsson, Lina Lindmark, Gudrun Hammarström, Marie-Louise Hammarström, Sten Sitohy, Basel Int J Mol Sci Article Chemokines are important in the development and progression of tumors. We investigated the expression of CXCL14 and CXCL16 in colon cancer. Expression of mRNA was assessed in primary tumors and lymph nodes and CXCL16 mRNA levels were correlated to patient’s survival. Protein expression was investigated by two-color immunofluorescence and immunomorphometry. CXCL14 and CXCL16 mRNA levels and protein expression were significantly higher in colon cancer primary tumors compared to apparently normal colon tissue. Positive cells were tumor cells, as revealed by anti-CEA and anti-EpCAM staining. CXCL16, but not CXCL14, mRNA levels were significantly higher in hematoxylin and eosin positive (H&E(+)) compared to H&E(−) colon cancer lymph nodes or control nodes (P < 0.0001). CXCL16 mRNA was expressed in 5/5 colon cancer cell lines while CXCL14 was expressed significantly in only one. Kaplan-Meier analysis revealed that colon cancer patients with lymph nodes expressing high or very high levels (7.2 and 11.4 copies/18S rRNA unit, respectively) of CXCL16 mRNA had a decreased mean survival time of 30 and 46 months at the 12-year follow-up (P = 0.04, P = 0.005, respectively). In conclusion, high expression of CXCL16 mRNA in regional lymph nodes of colon cancer patients is a sign of a poor prognosis. MDPI 2019-11-18 /pmc/articles/PMC6888697/ /pubmed/31752131 http://dx.doi.org/10.3390/ijms20225793 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
AbdelMageed, Manar
Ali, Haytham
Olsson, Lina
Lindmark, Gudrun
Hammarström, Marie-Louise
Hammarström, Sten
Sitohy, Basel
The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome
title The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome
title_full The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome
title_fullStr The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome
title_full_unstemmed The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome
title_short The Chemokine CXCL16 Is a New Biomarker for Lymph Node Analysis of Colon Cancer Outcome
title_sort chemokine cxcl16 is a new biomarker for lymph node analysis of colon cancer outcome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888697/
https://www.ncbi.nlm.nih.gov/pubmed/31752131
http://dx.doi.org/10.3390/ijms20225793
work_keys_str_mv AT abdelmageedmanar thechemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT alihaytham thechemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT olssonlina thechemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT lindmarkgudrun thechemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT hammarstrommarielouise thechemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT hammarstromsten thechemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT sitohybasel thechemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT abdelmageedmanar chemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT alihaytham chemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT olssonlina chemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT lindmarkgudrun chemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT hammarstrommarielouise chemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT hammarstromsten chemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome
AT sitohybasel chemokinecxcl16isanewbiomarkerforlymphnodeanalysisofcoloncanceroutcome