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Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle
The thick (myosin-containing) filaments of vertebrate skeletal muscle are arranged in a hexagonal lattice, interleaved with an array of thin (actin-containing) filaments with which they interact to produce contraction. X-ray diffraction and EM have shown that there are two types of thick filament la...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888752/ https://www.ncbi.nlm.nih.gov/pubmed/31699797 http://dx.doi.org/10.1085/jgp.201912460 |
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author | Ma, Weikang Lee, Kyoung Hwan Yang, Shixin Irving, Thomas C. Craig, Roger |
author_facet | Ma, Weikang Lee, Kyoung Hwan Yang, Shixin Irving, Thomas C. Craig, Roger |
author_sort | Ma, Weikang |
collection | PubMed |
description | The thick (myosin-containing) filaments of vertebrate skeletal muscle are arranged in a hexagonal lattice, interleaved with an array of thin (actin-containing) filaments with which they interact to produce contraction. X-ray diffraction and EM have shown that there are two types of thick filament lattice. In the simple lattice, all filaments have the same orientation about their long axis, while in the superlattice, nearest neighbors have rotations differing by 0° or 60°. Tetrapods (amphibians, reptiles, birds, and mammals) typically have only a superlattice, while the simple lattice is confined to fish. We have performed x-ray diffraction and electron microscopy of the soleus (SOL) and extensor digitorum longus (EDL) muscles of the rat and found that while the EDL has a superlattice as expected, the SOL has a simple lattice. The EDL and SOL of the rat are unusual in being essentially pure fast and slow muscles, respectively. The mixed fiber content of most tetrapod muscles and/or lattice disorder may explain why the simple lattice has not been apparent in these vertebrates before. This is supported by only weak simple lattice diffraction in the x-ray pattern of mouse SOL, which has a greater mix of fiber types than rat SOL. We conclude that the simple lattice might be common in tetrapods. The correlation between fiber type and filament lattice arrangement suggests that the lattice arrangement may contribute to the functional properties of a muscle. |
format | Online Article Text |
id | pubmed-6888752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-68887522020-06-02 Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle Ma, Weikang Lee, Kyoung Hwan Yang, Shixin Irving, Thomas C. Craig, Roger J Gen Physiol Research Articles The thick (myosin-containing) filaments of vertebrate skeletal muscle are arranged in a hexagonal lattice, interleaved with an array of thin (actin-containing) filaments with which they interact to produce contraction. X-ray diffraction and EM have shown that there are two types of thick filament lattice. In the simple lattice, all filaments have the same orientation about their long axis, while in the superlattice, nearest neighbors have rotations differing by 0° or 60°. Tetrapods (amphibians, reptiles, birds, and mammals) typically have only a superlattice, while the simple lattice is confined to fish. We have performed x-ray diffraction and electron microscopy of the soleus (SOL) and extensor digitorum longus (EDL) muscles of the rat and found that while the EDL has a superlattice as expected, the SOL has a simple lattice. The EDL and SOL of the rat are unusual in being essentially pure fast and slow muscles, respectively. The mixed fiber content of most tetrapod muscles and/or lattice disorder may explain why the simple lattice has not been apparent in these vertebrates before. This is supported by only weak simple lattice diffraction in the x-ray pattern of mouse SOL, which has a greater mix of fiber types than rat SOL. We conclude that the simple lattice might be common in tetrapods. The correlation between fiber type and filament lattice arrangement suggests that the lattice arrangement may contribute to the functional properties of a muscle. Rockefeller University Press 2019-12-02 2019-11-07 /pmc/articles/PMC6888752/ /pubmed/31699797 http://dx.doi.org/10.1085/jgp.201912460 Text en © 2019 Ma et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Ma, Weikang Lee, Kyoung Hwan Yang, Shixin Irving, Thomas C. Craig, Roger Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle |
title | Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle |
title_full | Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle |
title_fullStr | Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle |
title_full_unstemmed | Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle |
title_short | Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle |
title_sort | lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888752/ https://www.ncbi.nlm.nih.gov/pubmed/31699797 http://dx.doi.org/10.1085/jgp.201912460 |
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