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Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle

The thick (myosin-containing) filaments of vertebrate skeletal muscle are arranged in a hexagonal lattice, interleaved with an array of thin (actin-containing) filaments with which they interact to produce contraction. X-ray diffraction and EM have shown that there are two types of thick filament la...

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Autores principales: Ma, Weikang, Lee, Kyoung Hwan, Yang, Shixin, Irving, Thomas C., Craig, Roger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888752/
https://www.ncbi.nlm.nih.gov/pubmed/31699797
http://dx.doi.org/10.1085/jgp.201912460
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author Ma, Weikang
Lee, Kyoung Hwan
Yang, Shixin
Irving, Thomas C.
Craig, Roger
author_facet Ma, Weikang
Lee, Kyoung Hwan
Yang, Shixin
Irving, Thomas C.
Craig, Roger
author_sort Ma, Weikang
collection PubMed
description The thick (myosin-containing) filaments of vertebrate skeletal muscle are arranged in a hexagonal lattice, interleaved with an array of thin (actin-containing) filaments with which they interact to produce contraction. X-ray diffraction and EM have shown that there are two types of thick filament lattice. In the simple lattice, all filaments have the same orientation about their long axis, while in the superlattice, nearest neighbors have rotations differing by 0° or 60°. Tetrapods (amphibians, reptiles, birds, and mammals) typically have only a superlattice, while the simple lattice is confined to fish. We have performed x-ray diffraction and electron microscopy of the soleus (SOL) and extensor digitorum longus (EDL) muscles of the rat and found that while the EDL has a superlattice as expected, the SOL has a simple lattice. The EDL and SOL of the rat are unusual in being essentially pure fast and slow muscles, respectively. The mixed fiber content of most tetrapod muscles and/or lattice disorder may explain why the simple lattice has not been apparent in these vertebrates before. This is supported by only weak simple lattice diffraction in the x-ray pattern of mouse SOL, which has a greater mix of fiber types than rat SOL. We conclude that the simple lattice might be common in tetrapods. The correlation between fiber type and filament lattice arrangement suggests that the lattice arrangement may contribute to the functional properties of a muscle.
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spelling pubmed-68887522020-06-02 Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle Ma, Weikang Lee, Kyoung Hwan Yang, Shixin Irving, Thomas C. Craig, Roger J Gen Physiol Research Articles The thick (myosin-containing) filaments of vertebrate skeletal muscle are arranged in a hexagonal lattice, interleaved with an array of thin (actin-containing) filaments with which they interact to produce contraction. X-ray diffraction and EM have shown that there are two types of thick filament lattice. In the simple lattice, all filaments have the same orientation about their long axis, while in the superlattice, nearest neighbors have rotations differing by 0° or 60°. Tetrapods (amphibians, reptiles, birds, and mammals) typically have only a superlattice, while the simple lattice is confined to fish. We have performed x-ray diffraction and electron microscopy of the soleus (SOL) and extensor digitorum longus (EDL) muscles of the rat and found that while the EDL has a superlattice as expected, the SOL has a simple lattice. The EDL and SOL of the rat are unusual in being essentially pure fast and slow muscles, respectively. The mixed fiber content of most tetrapod muscles and/or lattice disorder may explain why the simple lattice has not been apparent in these vertebrates before. This is supported by only weak simple lattice diffraction in the x-ray pattern of mouse SOL, which has a greater mix of fiber types than rat SOL. We conclude that the simple lattice might be common in tetrapods. The correlation between fiber type and filament lattice arrangement suggests that the lattice arrangement may contribute to the functional properties of a muscle. Rockefeller University Press 2019-12-02 2019-11-07 /pmc/articles/PMC6888752/ /pubmed/31699797 http://dx.doi.org/10.1085/jgp.201912460 Text en © 2019 Ma et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Ma, Weikang
Lee, Kyoung Hwan
Yang, Shixin
Irving, Thomas C.
Craig, Roger
Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle
title Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle
title_full Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle
title_fullStr Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle
title_full_unstemmed Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle
title_short Lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle
title_sort lattice arrangement of myosin filaments correlates with fiber type in rat skeletal muscle
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888752/
https://www.ncbi.nlm.nih.gov/pubmed/31699797
http://dx.doi.org/10.1085/jgp.201912460
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