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The DEAD-Box RNA Helicase DDX1 Interacts with the Viral Protein 3D and Inhibits Foot-and-Mouth Disease Virus Replication

Foot-and-mouth disease virus (FMDV) can infect domestic and wild cloven-hoofed animals. The non-structural protein 3D plays an important role in FMDV replication and pathogenesis. However, the interaction partners of 3D, and the effects of those interactions on FMDV replication, remain incompletely...

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Autores principales: Xue, Qiao, Liu, Huisheng, Zeng, Qiaoying, Zheng, Haixue, Xue, Qinghong, Cai, Xuepeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888807/
https://www.ncbi.nlm.nih.gov/pubmed/31359346
http://dx.doi.org/10.1007/s12250-019-00148-7
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author Xue, Qiao
Liu, Huisheng
Zeng, Qiaoying
Zheng, Haixue
Xue, Qinghong
Cai, Xuepeng
author_facet Xue, Qiao
Liu, Huisheng
Zeng, Qiaoying
Zheng, Haixue
Xue, Qinghong
Cai, Xuepeng
author_sort Xue, Qiao
collection PubMed
description Foot-and-mouth disease virus (FMDV) can infect domestic and wild cloven-hoofed animals. The non-structural protein 3D plays an important role in FMDV replication and pathogenesis. However, the interaction partners of 3D, and the effects of those interactions on FMDV replication, remain incompletely elucidated. In the present study, using the yeast two-hybrid system, we identified a porcine cell protein, DEAD-box RNA helicase 1 (DDX1), which interacted with FMDV 3D. The DDX1-3D interaction was further confirmed by co-immunoprecipitation experiments and an indirect immunofluorescence assay (IFA) in porcine kidney 15 (PK-15) cells. DDX1 was reported to either inhibit or facilitate viral replication and regulate host innate immune responses. However, the roles of DDX1 during FMDV infection remain unclear. Our results revealed that DDX1 inhibited FMDV replication in an ATPase/helicase activity-dependent manner. In addition, DDX1 stimulated IFN-β activation in FMDV-infected cells. Together, our results expand the body of knowledge regarding the role of DDX1 in FMDV infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12250-019-00148-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-68888072020-03-24 The DEAD-Box RNA Helicase DDX1 Interacts with the Viral Protein 3D and Inhibits Foot-and-Mouth Disease Virus Replication Xue, Qiao Liu, Huisheng Zeng, Qiaoying Zheng, Haixue Xue, Qinghong Cai, Xuepeng Virol Sin Research Article Foot-and-mouth disease virus (FMDV) can infect domestic and wild cloven-hoofed animals. The non-structural protein 3D plays an important role in FMDV replication and pathogenesis. However, the interaction partners of 3D, and the effects of those interactions on FMDV replication, remain incompletely elucidated. In the present study, using the yeast two-hybrid system, we identified a porcine cell protein, DEAD-box RNA helicase 1 (DDX1), which interacted with FMDV 3D. The DDX1-3D interaction was further confirmed by co-immunoprecipitation experiments and an indirect immunofluorescence assay (IFA) in porcine kidney 15 (PK-15) cells. DDX1 was reported to either inhibit or facilitate viral replication and regulate host innate immune responses. However, the roles of DDX1 during FMDV infection remain unclear. Our results revealed that DDX1 inhibited FMDV replication in an ATPase/helicase activity-dependent manner. In addition, DDX1 stimulated IFN-β activation in FMDV-infected cells. Together, our results expand the body of knowledge regarding the role of DDX1 in FMDV infection. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12250-019-00148-7) contains supplementary material, which is available to authorized users. Springer Singapore 2019-07-29 /pmc/articles/PMC6888807/ /pubmed/31359346 http://dx.doi.org/10.1007/s12250-019-00148-7 Text en © Wuhan Institute of Virology, CAS 2019
spellingShingle Research Article
Xue, Qiao
Liu, Huisheng
Zeng, Qiaoying
Zheng, Haixue
Xue, Qinghong
Cai, Xuepeng
The DEAD-Box RNA Helicase DDX1 Interacts with the Viral Protein 3D and Inhibits Foot-and-Mouth Disease Virus Replication
title The DEAD-Box RNA Helicase DDX1 Interacts with the Viral Protein 3D and Inhibits Foot-and-Mouth Disease Virus Replication
title_full The DEAD-Box RNA Helicase DDX1 Interacts with the Viral Protein 3D and Inhibits Foot-and-Mouth Disease Virus Replication
title_fullStr The DEAD-Box RNA Helicase DDX1 Interacts with the Viral Protein 3D and Inhibits Foot-and-Mouth Disease Virus Replication
title_full_unstemmed The DEAD-Box RNA Helicase DDX1 Interacts with the Viral Protein 3D and Inhibits Foot-and-Mouth Disease Virus Replication
title_short The DEAD-Box RNA Helicase DDX1 Interacts with the Viral Protein 3D and Inhibits Foot-and-Mouth Disease Virus Replication
title_sort dead-box rna helicase ddx1 interacts with the viral protein 3d and inhibits foot-and-mouth disease virus replication
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888807/
https://www.ncbi.nlm.nih.gov/pubmed/31359346
http://dx.doi.org/10.1007/s12250-019-00148-7
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