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Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli

When nutrients run out, bacteria enter a dormant metabolic state. This low or undetectable metabolic activity helps bacteria to preserve their scant reserves for the future needs, yet it also diminishes their ability to scan the environment for new growth-promoting substrates. However, neighboring m...

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Autores principales: Jõers, Arvi, Vind, Kristiina, Hernández, Sara B., Maruste, Regina, Pereira, Marta, Brauer, Age, Remm, Maido, Cava, Felipe, Tenson, Tanel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888817/
https://www.ncbi.nlm.nih.gov/pubmed/31792329
http://dx.doi.org/10.1038/s41598-019-54646-5
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author Jõers, Arvi
Vind, Kristiina
Hernández, Sara B.
Maruste, Regina
Pereira, Marta
Brauer, Age
Remm, Maido
Cava, Felipe
Tenson, Tanel
author_facet Jõers, Arvi
Vind, Kristiina
Hernández, Sara B.
Maruste, Regina
Pereira, Marta
Brauer, Age
Remm, Maido
Cava, Felipe
Tenson, Tanel
author_sort Jõers, Arvi
collection PubMed
description When nutrients run out, bacteria enter a dormant metabolic state. This low or undetectable metabolic activity helps bacteria to preserve their scant reserves for the future needs, yet it also diminishes their ability to scan the environment for new growth-promoting substrates. However, neighboring microbial growth is a reliable indicator of a favorable environment and can thus serve as a cue for exiting dormancy. Here we report that for Escherichia coli and Pseudomonas aeruginosa this cue is provided by the basic peptidoglycan unit (i.e. muropeptide). We show that several forms of muropeptides from a variety of bacterial species can stimulate growth resumption of dormant cells and the sugar – peptide bond is crucial for this activity. These results, together with previous research that identifies muropeptides as a germination signal for bacterial spores, and their detection by mammalian immune cells, show that muropeptides are a universal cue for bacterial growth.
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spelling pubmed-68888172019-12-10 Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli Jõers, Arvi Vind, Kristiina Hernández, Sara B. Maruste, Regina Pereira, Marta Brauer, Age Remm, Maido Cava, Felipe Tenson, Tanel Sci Rep Article When nutrients run out, bacteria enter a dormant metabolic state. This low or undetectable metabolic activity helps bacteria to preserve their scant reserves for the future needs, yet it also diminishes their ability to scan the environment for new growth-promoting substrates. However, neighboring microbial growth is a reliable indicator of a favorable environment and can thus serve as a cue for exiting dormancy. Here we report that for Escherichia coli and Pseudomonas aeruginosa this cue is provided by the basic peptidoglycan unit (i.e. muropeptide). We show that several forms of muropeptides from a variety of bacterial species can stimulate growth resumption of dormant cells and the sugar – peptide bond is crucial for this activity. These results, together with previous research that identifies muropeptides as a germination signal for bacterial spores, and their detection by mammalian immune cells, show that muropeptides are a universal cue for bacterial growth. Nature Publishing Group UK 2019-12-02 /pmc/articles/PMC6888817/ /pubmed/31792329 http://dx.doi.org/10.1038/s41598-019-54646-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Jõers, Arvi
Vind, Kristiina
Hernández, Sara B.
Maruste, Regina
Pereira, Marta
Brauer, Age
Remm, Maido
Cava, Felipe
Tenson, Tanel
Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli
title Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli
title_full Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli
title_fullStr Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli
title_full_unstemmed Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli
title_short Muropeptides Stimulate Growth Resumption from Stationary Phase in Escherichia coli
title_sort muropeptides stimulate growth resumption from stationary phase in escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888817/
https://www.ncbi.nlm.nih.gov/pubmed/31792329
http://dx.doi.org/10.1038/s41598-019-54646-5
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