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Regulation of vanillate and syringate catabolism by a MarR-type transcriptional regulator DesR in Sphingobium sp. SYK-6

Vanillate and syringate are major intermediate metabolites generated during the microbial degradation of lignin. In Sphingobium sp. SYK-6, vanillate is O demethylated to protocatechuate by LigM; protocatechuate is then catabolized via the protocatechuate 4,5-cleavage pathway. Syringate is O demethyl...

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Autores principales: Araki, Takuma, Umeda, Shusuke, Kamimura, Naofumi, Kasai, Daisuke, Kumano, Shuta, Abe, Tomokuni, Kawazu, Chika, Otsuka, Yuichiro, Nakamura, Masaya, Katayama, Yoshihiro, Fukuda, Masao, Masai, Eiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888825/
https://www.ncbi.nlm.nih.gov/pubmed/31792252
http://dx.doi.org/10.1038/s41598-019-54490-7
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author Araki, Takuma
Umeda, Shusuke
Kamimura, Naofumi
Kasai, Daisuke
Kumano, Shuta
Abe, Tomokuni
Kawazu, Chika
Otsuka, Yuichiro
Nakamura, Masaya
Katayama, Yoshihiro
Fukuda, Masao
Masai, Eiji
author_facet Araki, Takuma
Umeda, Shusuke
Kamimura, Naofumi
Kasai, Daisuke
Kumano, Shuta
Abe, Tomokuni
Kawazu, Chika
Otsuka, Yuichiro
Nakamura, Masaya
Katayama, Yoshihiro
Fukuda, Masao
Masai, Eiji
author_sort Araki, Takuma
collection PubMed
description Vanillate and syringate are major intermediate metabolites generated during the microbial degradation of lignin. In Sphingobium sp. SYK-6, vanillate is O demethylated to protocatechuate by LigM; protocatechuate is then catabolized via the protocatechuate 4,5-cleavage pathway. Syringate is O demethylated to gallate by consecutive reactions catalyzed by DesA and LigM, and then gallate is subjected to ring cleavage by DesB. Here, we investigated the transcriptional regulation of desA, ligM, and desB involved in vanillate and syringate catabolism. Quantitative reverse transcription-PCR analyses indicated that the transcription of these genes was induced 5.8–37-fold in the presence of vanillate and syringate. A MarR-type transcriptional regulator, SLG_12870 (desR), was identified as the gene whose product bound to the desB promoter region. Analysis of a desR mutant indicated that the transcription of desB, ligM, and desR is negatively regulated by DesR. Purified DesR bound to the upstream regions of desB, ligM, and desR, and the inverted repeat sequences similar to each other in these regions were suggested to be essential for DNA binding of DesR. Vanillate and syringate inhibited DNA binding of DesR, indicating that these compounds are effector molecules of DesR. The transcription of desA was found to be regulated by an as-yet unidentified regulator.
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spelling pubmed-68888252019-12-10 Regulation of vanillate and syringate catabolism by a MarR-type transcriptional regulator DesR in Sphingobium sp. SYK-6 Araki, Takuma Umeda, Shusuke Kamimura, Naofumi Kasai, Daisuke Kumano, Shuta Abe, Tomokuni Kawazu, Chika Otsuka, Yuichiro Nakamura, Masaya Katayama, Yoshihiro Fukuda, Masao Masai, Eiji Sci Rep Article Vanillate and syringate are major intermediate metabolites generated during the microbial degradation of lignin. In Sphingobium sp. SYK-6, vanillate is O demethylated to protocatechuate by LigM; protocatechuate is then catabolized via the protocatechuate 4,5-cleavage pathway. Syringate is O demethylated to gallate by consecutive reactions catalyzed by DesA and LigM, and then gallate is subjected to ring cleavage by DesB. Here, we investigated the transcriptional regulation of desA, ligM, and desB involved in vanillate and syringate catabolism. Quantitative reverse transcription-PCR analyses indicated that the transcription of these genes was induced 5.8–37-fold in the presence of vanillate and syringate. A MarR-type transcriptional regulator, SLG_12870 (desR), was identified as the gene whose product bound to the desB promoter region. Analysis of a desR mutant indicated that the transcription of desB, ligM, and desR is negatively regulated by DesR. Purified DesR bound to the upstream regions of desB, ligM, and desR, and the inverted repeat sequences similar to each other in these regions were suggested to be essential for DNA binding of DesR. Vanillate and syringate inhibited DNA binding of DesR, indicating that these compounds are effector molecules of DesR. The transcription of desA was found to be regulated by an as-yet unidentified regulator. Nature Publishing Group UK 2019-12-02 /pmc/articles/PMC6888825/ /pubmed/31792252 http://dx.doi.org/10.1038/s41598-019-54490-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Araki, Takuma
Umeda, Shusuke
Kamimura, Naofumi
Kasai, Daisuke
Kumano, Shuta
Abe, Tomokuni
Kawazu, Chika
Otsuka, Yuichiro
Nakamura, Masaya
Katayama, Yoshihiro
Fukuda, Masao
Masai, Eiji
Regulation of vanillate and syringate catabolism by a MarR-type transcriptional regulator DesR in Sphingobium sp. SYK-6
title Regulation of vanillate and syringate catabolism by a MarR-type transcriptional regulator DesR in Sphingobium sp. SYK-6
title_full Regulation of vanillate and syringate catabolism by a MarR-type transcriptional regulator DesR in Sphingobium sp. SYK-6
title_fullStr Regulation of vanillate and syringate catabolism by a MarR-type transcriptional regulator DesR in Sphingobium sp. SYK-6
title_full_unstemmed Regulation of vanillate and syringate catabolism by a MarR-type transcriptional regulator DesR in Sphingobium sp. SYK-6
title_short Regulation of vanillate and syringate catabolism by a MarR-type transcriptional regulator DesR in Sphingobium sp. SYK-6
title_sort regulation of vanillate and syringate catabolism by a marr-type transcriptional regulator desr in sphingobium sp. syk-6
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6888825/
https://www.ncbi.nlm.nih.gov/pubmed/31792252
http://dx.doi.org/10.1038/s41598-019-54490-7
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