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Influence of gallic and tannic acid on therapeutic properties of acarbose in vitro and in vivo in Drosophila melanogaster
BACKGROUND: In this study, gallic acid (GA) and its polymeric form-tannic acid (TA) which are two phenolic acids found abundantly distributed in plant food sources were investigated for their influence on therapeutic properties of acarbose (AC) in vitro and in vivo in Drosophila melanogaster. METHOD...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Chang Gung University
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889231/ https://www.ncbi.nlm.nih.gov/pubmed/31783992 http://dx.doi.org/10.1016/j.bj.2019.01.005 |
Sumario: | BACKGROUND: In this study, gallic acid (GA) and its polymeric form-tannic acid (TA) which are two phenolic acids found abundantly distributed in plant food sources were investigated for their influence on therapeutic properties of acarbose (AC) in vitro and in vivo in Drosophila melanogaster. METHODS: Combinations of AC and GA or TA were assessed for their alpha-glucosidase and alpha-amylase inhibitory effects as markers of anti-hyperglycemic properties, as well as their free radicals scavenging, Fe(2+) chelating and malondialdehyde (MDA) inhibitory effects (in vitro). Furthermore, wild type D. melanogaster cultures were raised on diets containing AC, GA, TA and their various combinations for seven days. Thereafter, flies were homogenized and glucose concentrations, alpha-glucosidase and alpha-amylase activities, as well as reactive oxygen species (ROS) and total thiol levels were determined. RESULTS: The results showed that GA and TA up to 5 mg/ml significantly (p < 0.05) increased the enzymes' inhibitory effects and antioxidant properties of AC in vitro. Also, there was significant reduction in glucose concentration, enzyme activities and ROS level in D. melanogaster fed diets supplemented with phenolic acids and acarbose. CONCLUSIONS: These bioactive compounds–drug interactions provide useful information on improving the therapeutic properties of acarbose especially in its use as an antidiabetic drug. |
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