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Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles

There are disease-causing biohazards in the blood that cannot be treated with modern medicines. Here we show that an intelligently designed safe biomaterial can precisely identify, tow and dump a targeted biohazard from the blood into the small intestine. Positively charged mesoporous silica nanopar...

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Autores principales: Xie, Xiaodong, Nie, Huifang, Zhou, Yu, Lian, Shu, Mei, Hao, Lu, Yusheng, Dong, Haiyan, Li, Fengqiao, Li, Tao, Li, Bifei, Wang, Jie, Lin, Min, Wang, Chaihung, Shao, Jingwei, Gao, Yu, Chen, Jianming, Xie, Fangwei, Jia, Lee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889386/
https://www.ncbi.nlm.nih.gov/pubmed/31792209
http://dx.doi.org/10.1038/s41467-019-13316-w
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author Xie, Xiaodong
Nie, Huifang
Zhou, Yu
Lian, Shu
Mei, Hao
Lu, Yusheng
Dong, Haiyan
Li, Fengqiao
Li, Tao
Li, Bifei
Wang, Jie
Lin, Min
Wang, Chaihung
Shao, Jingwei
Gao, Yu
Chen, Jianming
Xie, Fangwei
Jia, Lee
author_facet Xie, Xiaodong
Nie, Huifang
Zhou, Yu
Lian, Shu
Mei, Hao
Lu, Yusheng
Dong, Haiyan
Li, Fengqiao
Li, Tao
Li, Bifei
Wang, Jie
Lin, Min
Wang, Chaihung
Shao, Jingwei
Gao, Yu
Chen, Jianming
Xie, Fangwei
Jia, Lee
author_sort Xie, Xiaodong
collection PubMed
description There are disease-causing biohazards in the blood that cannot be treated with modern medicines. Here we show that an intelligently designed safe biomaterial can precisely identify, tow and dump a targeted biohazard from the blood into the small intestine. Positively charged mesoporous silica nanoparticles (MSNs) functionalized with EGFR-targeting aptamers (MSN-AP) specifically recognize and bind blood-borne negatively charged oncogenic exosomes (A-Exo), and tow A-Exo across hepatobiliary layers and Oddi’s sphincter into the small intestine. MSN-AP specifically distinguish and bind A-Exo from interfering exosomes in cell culture and rat and patient blood to form MSN-AP and A-Exo conjugates (MSN-Exo) that transverse hepatocytes, cholangiocytes, and endothelial monolayers via endocytosis and exocytosis mechanisms, although Kupffer cells have been shown to engulf some MSN-Exo. Blood MSN-AP significantly decreased circulating A-Exo levels, sequentially increased intestinal A-Exo and attenuated A-Exo-induced lung metastasis in mice. This study opens an innovative avenue to relocate blood-borne life-threatening biohazards to the intestine.
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spelling pubmed-68893862019-12-04 Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles Xie, Xiaodong Nie, Huifang Zhou, Yu Lian, Shu Mei, Hao Lu, Yusheng Dong, Haiyan Li, Fengqiao Li, Tao Li, Bifei Wang, Jie Lin, Min Wang, Chaihung Shao, Jingwei Gao, Yu Chen, Jianming Xie, Fangwei Jia, Lee Nat Commun Article There are disease-causing biohazards in the blood that cannot be treated with modern medicines. Here we show that an intelligently designed safe biomaterial can precisely identify, tow and dump a targeted biohazard from the blood into the small intestine. Positively charged mesoporous silica nanoparticles (MSNs) functionalized with EGFR-targeting aptamers (MSN-AP) specifically recognize and bind blood-borne negatively charged oncogenic exosomes (A-Exo), and tow A-Exo across hepatobiliary layers and Oddi’s sphincter into the small intestine. MSN-AP specifically distinguish and bind A-Exo from interfering exosomes in cell culture and rat and patient blood to form MSN-AP and A-Exo conjugates (MSN-Exo) that transverse hepatocytes, cholangiocytes, and endothelial monolayers via endocytosis and exocytosis mechanisms, although Kupffer cells have been shown to engulf some MSN-Exo. Blood MSN-AP significantly decreased circulating A-Exo levels, sequentially increased intestinal A-Exo and attenuated A-Exo-induced lung metastasis in mice. This study opens an innovative avenue to relocate blood-borne life-threatening biohazards to the intestine. Nature Publishing Group UK 2019-12-02 /pmc/articles/PMC6889386/ /pubmed/31792209 http://dx.doi.org/10.1038/s41467-019-13316-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xie, Xiaodong
Nie, Huifang
Zhou, Yu
Lian, Shu
Mei, Hao
Lu, Yusheng
Dong, Haiyan
Li, Fengqiao
Li, Tao
Li, Bifei
Wang, Jie
Lin, Min
Wang, Chaihung
Shao, Jingwei
Gao, Yu
Chen, Jianming
Xie, Fangwei
Jia, Lee
Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles
title Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles
title_full Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles
title_fullStr Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles
title_full_unstemmed Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles
title_short Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles
title_sort eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889386/
https://www.ncbi.nlm.nih.gov/pubmed/31792209
http://dx.doi.org/10.1038/s41467-019-13316-w
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