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Exosomes Carrying MicroRNA-155 Target Forkhead Box O3 of Endothelial Cells and Promote Angiogenesis in Gastric Cancer

Gastric cancer (GC) has a poor prognosis due to its relentless proliferation and metastasis. One of the reasons for this plight is the formidable angiogenesis ability of GC. Considering the important role of cancer exosomes as carriers and communicators in the tumor microenvironment, we explored the...

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Autores principales: Zhou, Zhengyang, Zhang, Haiyang, Deng, Ting, Ning, Tao, Liu, Rui, Liu, Dongying, Bai, Ming, Ying, Guoguang, Ba, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889542/
https://www.ncbi.nlm.nih.gov/pubmed/31828217
http://dx.doi.org/10.1016/j.omto.2019.10.006
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author Zhou, Zhengyang
Zhang, Haiyang
Deng, Ting
Ning, Tao
Liu, Rui
Liu, Dongying
Bai, Ming
Ying, Guoguang
Ba, Yi
author_facet Zhou, Zhengyang
Zhang, Haiyang
Deng, Ting
Ning, Tao
Liu, Rui
Liu, Dongying
Bai, Ming
Ying, Guoguang
Ba, Yi
author_sort Zhou, Zhengyang
collection PubMed
description Gastric cancer (GC) has a poor prognosis due to its relentless proliferation and metastasis. One of the reasons for this plight is the formidable angiogenesis ability of GC. Considering the important role of cancer exosomes as carriers and communicators in the tumor microenvironment, we explored the role of exosome-microRNA (miRNA) in regulating angiogenesis. Western blotting and quantitative real-time PCR were used to measure the protein and mRNA levels of the miRNA target gene. To detect changes in cellular biological functions, we pretreated human umbilical vein endothelial cells (HUVECs) that were severally cocultured with GC-derived exosomes and transfected them with different miRNAs directly. Also, we used the mouse xenograft model to verify the effect of miR-155 on angiogenesis of GC tissues in vivo. Our study confirmed that miR-155, as a driver of angiogenesis, encapsulated by exosomes from GC can enhance the generation of new vessels for GC in vitro through inhibiting the expression of Forkhead box O3 (FOXO3a) protein, which led to the progression of GC. Therefore, miR-155 is probable to become a potential biomarker for the detection of migration and angiogenesis of GC, and serves as a novel target for anti-angiogenesis therapy.
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spelling pubmed-68895422019-12-11 Exosomes Carrying MicroRNA-155 Target Forkhead Box O3 of Endothelial Cells and Promote Angiogenesis in Gastric Cancer Zhou, Zhengyang Zhang, Haiyang Deng, Ting Ning, Tao Liu, Rui Liu, Dongying Bai, Ming Ying, Guoguang Ba, Yi Mol Ther Oncolytics Article Gastric cancer (GC) has a poor prognosis due to its relentless proliferation and metastasis. One of the reasons for this plight is the formidable angiogenesis ability of GC. Considering the important role of cancer exosomes as carriers and communicators in the tumor microenvironment, we explored the role of exosome-microRNA (miRNA) in regulating angiogenesis. Western blotting and quantitative real-time PCR were used to measure the protein and mRNA levels of the miRNA target gene. To detect changes in cellular biological functions, we pretreated human umbilical vein endothelial cells (HUVECs) that were severally cocultured with GC-derived exosomes and transfected them with different miRNAs directly. Also, we used the mouse xenograft model to verify the effect of miR-155 on angiogenesis of GC tissues in vivo. Our study confirmed that miR-155, as a driver of angiogenesis, encapsulated by exosomes from GC can enhance the generation of new vessels for GC in vitro through inhibiting the expression of Forkhead box O3 (FOXO3a) protein, which led to the progression of GC. Therefore, miR-155 is probable to become a potential biomarker for the detection of migration and angiogenesis of GC, and serves as a novel target for anti-angiogenesis therapy. American Society of Gene & Cell Therapy 2019-10-31 /pmc/articles/PMC6889542/ /pubmed/31828217 http://dx.doi.org/10.1016/j.omto.2019.10.006 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Zhou, Zhengyang
Zhang, Haiyang
Deng, Ting
Ning, Tao
Liu, Rui
Liu, Dongying
Bai, Ming
Ying, Guoguang
Ba, Yi
Exosomes Carrying MicroRNA-155 Target Forkhead Box O3 of Endothelial Cells and Promote Angiogenesis in Gastric Cancer
title Exosomes Carrying MicroRNA-155 Target Forkhead Box O3 of Endothelial Cells and Promote Angiogenesis in Gastric Cancer
title_full Exosomes Carrying MicroRNA-155 Target Forkhead Box O3 of Endothelial Cells and Promote Angiogenesis in Gastric Cancer
title_fullStr Exosomes Carrying MicroRNA-155 Target Forkhead Box O3 of Endothelial Cells and Promote Angiogenesis in Gastric Cancer
title_full_unstemmed Exosomes Carrying MicroRNA-155 Target Forkhead Box O3 of Endothelial Cells and Promote Angiogenesis in Gastric Cancer
title_short Exosomes Carrying MicroRNA-155 Target Forkhead Box O3 of Endothelial Cells and Promote Angiogenesis in Gastric Cancer
title_sort exosomes carrying microrna-155 target forkhead box o3 of endothelial cells and promote angiogenesis in gastric cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889542/
https://www.ncbi.nlm.nih.gov/pubmed/31828217
http://dx.doi.org/10.1016/j.omto.2019.10.006
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