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Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1

We replicated the rat common carotid artery (CCA) intima hyperplasia model and found the expression of a circular RNA, circRNA_009723 (circDcbld1), was markedly increased in the CCA with intimal hyperplasia. In vitro, the suppression of circDcbld1 in rat vascular smooth muscle cells (VSMCs) led the...

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Autores principales: Rong, Zhi-Hua, Chang, Neng-Bin, Yao, Qing-Ping, Li, Tao, Zhu, Xiao-Ling, Cao, Yu, Jiang, Mei-Jun, Cheng, Yan-Shuang, Jiang, Rui, Jiang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889766/
https://www.ncbi.nlm.nih.gov/pubmed/31778958
http://dx.doi.org/10.1016/j.omtn.2019.10.023
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author Rong, Zhi-Hua
Chang, Neng-Bin
Yao, Qing-Ping
Li, Tao
Zhu, Xiao-Ling
Cao, Yu
Jiang, Mei-Jun
Cheng, Yan-Shuang
Jiang, Rui
Jiang, Jun
author_facet Rong, Zhi-Hua
Chang, Neng-Bin
Yao, Qing-Ping
Li, Tao
Zhu, Xiao-Ling
Cao, Yu
Jiang, Mei-Jun
Cheng, Yan-Shuang
Jiang, Rui
Jiang, Jun
author_sort Rong, Zhi-Hua
collection PubMed
description We replicated the rat common carotid artery (CCA) intima hyperplasia model and found the expression of a circular RNA, circRNA_009723 (circDcbld1), was markedly increased in the CCA with intimal hyperplasia. In vitro, the suppression of circDcbld1 in rat vascular smooth muscle cells (VSMCs) led the increase of contractile smooth muscle cell markers and the decrease of cell migration. In vivo, the injection of chemically modified circDcbld1 small interfering RNA (siRNA) lessened the formation of neointima in rat CCA after balloon injury. Further experiments proved that circDcbld1, as a competing endogenous RNA, interacted with miR-145-3p and upregulated the level of neuropilin-1 (Nrp1), thereby regulating the migration of VSMCs. In this study, we demonstrated a new mechanism by which circular RNA promotes intimal hyperplasia. We deem that intervention in the circDcbld1-miR-145-3p/Nrp1 pathway might be a feasible approach to alleviate the post-injury intimal hyperplasia.
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spelling pubmed-68897662019-12-11 Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1 Rong, Zhi-Hua Chang, Neng-Bin Yao, Qing-Ping Li, Tao Zhu, Xiao-Ling Cao, Yu Jiang, Mei-Jun Cheng, Yan-Shuang Jiang, Rui Jiang, Jun Mol Ther Nucleic Acids Article We replicated the rat common carotid artery (CCA) intima hyperplasia model and found the expression of a circular RNA, circRNA_009723 (circDcbld1), was markedly increased in the CCA with intimal hyperplasia. In vitro, the suppression of circDcbld1 in rat vascular smooth muscle cells (VSMCs) led the increase of contractile smooth muscle cell markers and the decrease of cell migration. In vivo, the injection of chemically modified circDcbld1 small interfering RNA (siRNA) lessened the formation of neointima in rat CCA after balloon injury. Further experiments proved that circDcbld1, as a competing endogenous RNA, interacted with miR-145-3p and upregulated the level of neuropilin-1 (Nrp1), thereby regulating the migration of VSMCs. In this study, we demonstrated a new mechanism by which circular RNA promotes intimal hyperplasia. We deem that intervention in the circDcbld1-miR-145-3p/Nrp1 pathway might be a feasible approach to alleviate the post-injury intimal hyperplasia. American Society of Gene & Cell Therapy 2019-10-28 /pmc/articles/PMC6889766/ /pubmed/31778958 http://dx.doi.org/10.1016/j.omtn.2019.10.023 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Rong, Zhi-Hua
Chang, Neng-Bin
Yao, Qing-Ping
Li, Tao
Zhu, Xiao-Ling
Cao, Yu
Jiang, Mei-Jun
Cheng, Yan-Shuang
Jiang, Rui
Jiang, Jun
Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1
title Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1
title_full Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1
title_fullStr Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1
title_full_unstemmed Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1
title_short Suppression of circDcbld1 Alleviates Intimal Hyperplasia in Rat Carotid Artery by Targeting miR-145-3p/Neuropilin-1
title_sort suppression of circdcbld1 alleviates intimal hyperplasia in rat carotid artery by targeting mir-145-3p/neuropilin-1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889766/
https://www.ncbi.nlm.nih.gov/pubmed/31778958
http://dx.doi.org/10.1016/j.omtn.2019.10.023
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