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Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging
Human brain imaging studies have revealed several regions that are activated in patients with chronic pain. In rodent brains, functional changes due to chronic pain have not been fully elucidated, as brain imaging techniques such as functional magnetic resonance imaging and positron emission tomogra...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889800/ https://www.ncbi.nlm.nih.gov/pubmed/31849617 http://dx.doi.org/10.3389/fncir.2019.00074 |
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author | Inami, Chihiro Tanihira, Hiroki Kikuta, Satomi Ogasawara, Osamu Sobue, Kazuya Kume, Kazuhiko Osanai, Makoto Ohsawa, Masahiro |
author_facet | Inami, Chihiro Tanihira, Hiroki Kikuta, Satomi Ogasawara, Osamu Sobue, Kazuya Kume, Kazuhiko Osanai, Makoto Ohsawa, Masahiro |
author_sort | Inami, Chihiro |
collection | PubMed |
description | Human brain imaging studies have revealed several regions that are activated in patients with chronic pain. In rodent brains, functional changes due to chronic pain have not been fully elucidated, as brain imaging techniques such as functional magnetic resonance imaging and positron emission tomography (PET) require the use of anesthesia to suppress movement. Consequently, conclusions derived from existing imaging studies in rodents may not accurately reflect brain activity under awake conditions. In this study, we used quantitative activation-induced manganese-enhanced magnetic resonance imaging to directly capture the previous brain activity of awake mice. We also observed and quantified the brain activity of the spared nerve injury (SNI) neuropathic pain model during awake conditions. SNI-operated mice exhibited a robust decrease of mechanical nociceptive threshold 14 days after nerve injury. Imaging on SNI-operated mice revealed increased neural activity in the limbic system and secondary somatosensory, sensory-motor, piriform, and insular cortex. We present the first study demonstrating a direct measurement of awake neural activity in a neuropathic pain mouse model. |
format | Online Article Text |
id | pubmed-6889800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68898002019-12-17 Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging Inami, Chihiro Tanihira, Hiroki Kikuta, Satomi Ogasawara, Osamu Sobue, Kazuya Kume, Kazuhiko Osanai, Makoto Ohsawa, Masahiro Front Neural Circuits Neuroscience Human brain imaging studies have revealed several regions that are activated in patients with chronic pain. In rodent brains, functional changes due to chronic pain have not been fully elucidated, as brain imaging techniques such as functional magnetic resonance imaging and positron emission tomography (PET) require the use of anesthesia to suppress movement. Consequently, conclusions derived from existing imaging studies in rodents may not accurately reflect brain activity under awake conditions. In this study, we used quantitative activation-induced manganese-enhanced magnetic resonance imaging to directly capture the previous brain activity of awake mice. We also observed and quantified the brain activity of the spared nerve injury (SNI) neuropathic pain model during awake conditions. SNI-operated mice exhibited a robust decrease of mechanical nociceptive threshold 14 days after nerve injury. Imaging on SNI-operated mice revealed increased neural activity in the limbic system and secondary somatosensory, sensory-motor, piriform, and insular cortex. We present the first study demonstrating a direct measurement of awake neural activity in a neuropathic pain mouse model. Frontiers Media S.A. 2019-11-26 /pmc/articles/PMC6889800/ /pubmed/31849617 http://dx.doi.org/10.3389/fncir.2019.00074 Text en Copyright © 2019 Inami, Tanihira, Kikuta, Ogasawara, Sobue, Kume, Osanai and Ohsawa. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Inami, Chihiro Tanihira, Hiroki Kikuta, Satomi Ogasawara, Osamu Sobue, Kazuya Kume, Kazuhiko Osanai, Makoto Ohsawa, Masahiro Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
title | Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
title_full | Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
title_fullStr | Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
title_full_unstemmed | Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
title_short | Visualization of Brain Activity in a Neuropathic Pain Model Using Quantitative Activity-Dependent Manganese Magnetic Resonance Imaging |
title_sort | visualization of brain activity in a neuropathic pain model using quantitative activity-dependent manganese magnetic resonance imaging |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6889800/ https://www.ncbi.nlm.nih.gov/pubmed/31849617 http://dx.doi.org/10.3389/fncir.2019.00074 |
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