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Culex quinquefasciatus Egg Membrane Alteration and Ovicidal Activity of Cipadessa baccifera (Roth) Plant Extracts Compared to Synthetic Insect Growth Regulators

BACKGROUND: Insecticide resistance among mosquito vectors for synthetic insecticides still remains a major problem for control efforts. This study assessed the ovicidal potential of crude solvent extracts from the medicinal plant Cipadessa baccifera comparatively to standard registered synthetic ins...

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Autores principales: Ramkumar, Govindaraju, Karthi, Sengodan, Shivakumar, Muthugounder S, Kweka, Eliningaya J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890224/
https://www.ncbi.nlm.nih.gov/pubmed/31819700
http://dx.doi.org/10.2147/RRTM.S227590
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author Ramkumar, Govindaraju
Karthi, Sengodan
Shivakumar, Muthugounder S
Kweka, Eliningaya J
author_facet Ramkumar, Govindaraju
Karthi, Sengodan
Shivakumar, Muthugounder S
Kweka, Eliningaya J
author_sort Ramkumar, Govindaraju
collection PubMed
description BACKGROUND: Insecticide resistance among mosquito vectors for synthetic insecticides still remains a major problem for control efforts. This study assessed the ovicidal potential of crude solvent extracts from the medicinal plant Cipadessa baccifera comparatively to standard registered synthetic insect growth regulators (IGR) on freshly laid eggs of Culex quinquefasciatus. METHOD: Five plant extracts were prepared using different solvents. The batches of eggs were exposed to different concentrations of each solvent extract comparatively to synthetic IGR. The hatched eggs of Cx. quinquefasciatus were subjected to different concentrations. The first instars that emerged from the eggs were counted daily. The egg hatching inhibition was observed 24, 48 and 72 hrs post treatment. The desiccation median time (DT(50) and DT(90)) was calculated. RESULTS: The percent egg hatching inhibition was inversely proportional to the concentration of extracts. The morphological damage to the eggs was observed. Among five solvent extracts, acetone extracts showed the highest ovicidal activity. The changes in eggshell morphology were observed. The maximum ovicidal activity was observed in acetone extracts with DT(50) value of 1.70 hrs (0.91–2.22). The methanol plant extract using gas chromatography-mass spectrometry identified 14 compounds. CONCLUSION: These results suggest that the acetone extracts of C. baccifera have the potential to be used as an ovicidal agent for controlling mosquito populations in aquatic stages. The biodegradability of the extracts has the advantage of being eco-friendly.
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spelling pubmed-68902242019-12-09 Culex quinquefasciatus Egg Membrane Alteration and Ovicidal Activity of Cipadessa baccifera (Roth) Plant Extracts Compared to Synthetic Insect Growth Regulators Ramkumar, Govindaraju Karthi, Sengodan Shivakumar, Muthugounder S Kweka, Eliningaya J Res Rep Trop Med Original Research BACKGROUND: Insecticide resistance among mosquito vectors for synthetic insecticides still remains a major problem for control efforts. This study assessed the ovicidal potential of crude solvent extracts from the medicinal plant Cipadessa baccifera comparatively to standard registered synthetic insect growth regulators (IGR) on freshly laid eggs of Culex quinquefasciatus. METHOD: Five plant extracts were prepared using different solvents. The batches of eggs were exposed to different concentrations of each solvent extract comparatively to synthetic IGR. The hatched eggs of Cx. quinquefasciatus were subjected to different concentrations. The first instars that emerged from the eggs were counted daily. The egg hatching inhibition was observed 24, 48 and 72 hrs post treatment. The desiccation median time (DT(50) and DT(90)) was calculated. RESULTS: The percent egg hatching inhibition was inversely proportional to the concentration of extracts. The morphological damage to the eggs was observed. Among five solvent extracts, acetone extracts showed the highest ovicidal activity. The changes in eggshell morphology were observed. The maximum ovicidal activity was observed in acetone extracts with DT(50) value of 1.70 hrs (0.91–2.22). The methanol plant extract using gas chromatography-mass spectrometry identified 14 compounds. CONCLUSION: These results suggest that the acetone extracts of C. baccifera have the potential to be used as an ovicidal agent for controlling mosquito populations in aquatic stages. The biodegradability of the extracts has the advantage of being eco-friendly. Dove 2019-11-29 /pmc/articles/PMC6890224/ /pubmed/31819700 http://dx.doi.org/10.2147/RRTM.S227590 Text en © 2019 Ramkumar et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Ramkumar, Govindaraju
Karthi, Sengodan
Shivakumar, Muthugounder S
Kweka, Eliningaya J
Culex quinquefasciatus Egg Membrane Alteration and Ovicidal Activity of Cipadessa baccifera (Roth) Plant Extracts Compared to Synthetic Insect Growth Regulators
title Culex quinquefasciatus Egg Membrane Alteration and Ovicidal Activity of Cipadessa baccifera (Roth) Plant Extracts Compared to Synthetic Insect Growth Regulators
title_full Culex quinquefasciatus Egg Membrane Alteration and Ovicidal Activity of Cipadessa baccifera (Roth) Plant Extracts Compared to Synthetic Insect Growth Regulators
title_fullStr Culex quinquefasciatus Egg Membrane Alteration and Ovicidal Activity of Cipadessa baccifera (Roth) Plant Extracts Compared to Synthetic Insect Growth Regulators
title_full_unstemmed Culex quinquefasciatus Egg Membrane Alteration and Ovicidal Activity of Cipadessa baccifera (Roth) Plant Extracts Compared to Synthetic Insect Growth Regulators
title_short Culex quinquefasciatus Egg Membrane Alteration and Ovicidal Activity of Cipadessa baccifera (Roth) Plant Extracts Compared to Synthetic Insect Growth Regulators
title_sort culex quinquefasciatus egg membrane alteration and ovicidal activity of cipadessa baccifera (roth) plant extracts compared to synthetic insect growth regulators
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890224/
https://www.ncbi.nlm.nih.gov/pubmed/31819700
http://dx.doi.org/10.2147/RRTM.S227590
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