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βC1, pathogenicity determinant encoded by Cotton leaf curl Multan betasatellite, interacts with calmodulin-like protein 11 (Gh-CML11) in Gossypium hirsutum

Begomoviruses interfere with host plant machinery to evade host defense mechanism by interacting with plant proteins. In the old world, this group of viruses are usually associated with betasatellite that induces severe disease symptoms by encoding a protein, βC1, which is a pathogenicity determinan...

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Autores principales: Kamal, Hira, Minhas, Fayyaz-ul-Amir Afsar, Tripathi, Diwaker, Abbasi, Wajid Arshad, Hamza, Muhammad, Mustafa, Roma, Khan, Muhammad Zuhaib, Mansoor, Shahid, Pappu, Hanu R., Amin, Imran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890265/
https://www.ncbi.nlm.nih.gov/pubmed/31794580
http://dx.doi.org/10.1371/journal.pone.0225876
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author Kamal, Hira
Minhas, Fayyaz-ul-Amir Afsar
Tripathi, Diwaker
Abbasi, Wajid Arshad
Hamza, Muhammad
Mustafa, Roma
Khan, Muhammad Zuhaib
Mansoor, Shahid
Pappu, Hanu R.
Amin, Imran
author_facet Kamal, Hira
Minhas, Fayyaz-ul-Amir Afsar
Tripathi, Diwaker
Abbasi, Wajid Arshad
Hamza, Muhammad
Mustafa, Roma
Khan, Muhammad Zuhaib
Mansoor, Shahid
Pappu, Hanu R.
Amin, Imran
author_sort Kamal, Hira
collection PubMed
description Begomoviruses interfere with host plant machinery to evade host defense mechanism by interacting with plant proteins. In the old world, this group of viruses are usually associated with betasatellite that induces severe disease symptoms by encoding a protein, βC1, which is a pathogenicity determinant. Here, we show that βC1 encoded by Cotton leaf curl Multan betasatellite (CLCuMB) requires Gossypium hirsutum calmodulin-like protein 11 (Gh-CML11) to infect cotton. First, we used the in silico approach to predict the interaction of CLCuMB-βC1 with Gh-CML11. A number of sequence- and structure-based in-silico interaction prediction techniques suggested a strong putative binding of CLCuMB-βC1 with Gh-CML11 in a Ca(+2)-dependent manner. In-silico interaction prediction was then confirmed by three different experimental approaches: The Gh-CML11 interaction was confirmed using CLCuMB-βC1 in a yeast two hybrid system and pull down assay. These results were further validated using bimolecular fluorescence complementation system showing the interaction in cytoplasmic veins of Nicotiana benthamiana. Bioinformatics and molecular studies suggested that CLCuMB-βC1 induces the overexpression of Gh-CML11 protein and ultimately provides calcium as a nutrient source for virus movement and transmission. This is the first comprehensive study on the interaction between CLCuMB-βC1 and Gh-CML11 proteins which provided insights into our understating of the role of βC1 in cotton leaf curl disease.
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spelling pubmed-68902652019-12-13 βC1, pathogenicity determinant encoded by Cotton leaf curl Multan betasatellite, interacts with calmodulin-like protein 11 (Gh-CML11) in Gossypium hirsutum Kamal, Hira Minhas, Fayyaz-ul-Amir Afsar Tripathi, Diwaker Abbasi, Wajid Arshad Hamza, Muhammad Mustafa, Roma Khan, Muhammad Zuhaib Mansoor, Shahid Pappu, Hanu R. Amin, Imran PLoS One Research Article Begomoviruses interfere with host plant machinery to evade host defense mechanism by interacting with plant proteins. In the old world, this group of viruses are usually associated with betasatellite that induces severe disease symptoms by encoding a protein, βC1, which is a pathogenicity determinant. Here, we show that βC1 encoded by Cotton leaf curl Multan betasatellite (CLCuMB) requires Gossypium hirsutum calmodulin-like protein 11 (Gh-CML11) to infect cotton. First, we used the in silico approach to predict the interaction of CLCuMB-βC1 with Gh-CML11. A number of sequence- and structure-based in-silico interaction prediction techniques suggested a strong putative binding of CLCuMB-βC1 with Gh-CML11 in a Ca(+2)-dependent manner. In-silico interaction prediction was then confirmed by three different experimental approaches: The Gh-CML11 interaction was confirmed using CLCuMB-βC1 in a yeast two hybrid system and pull down assay. These results were further validated using bimolecular fluorescence complementation system showing the interaction in cytoplasmic veins of Nicotiana benthamiana. Bioinformatics and molecular studies suggested that CLCuMB-βC1 induces the overexpression of Gh-CML11 protein and ultimately provides calcium as a nutrient source for virus movement and transmission. This is the first comprehensive study on the interaction between CLCuMB-βC1 and Gh-CML11 proteins which provided insights into our understating of the role of βC1 in cotton leaf curl disease. Public Library of Science 2019-12-03 /pmc/articles/PMC6890265/ /pubmed/31794580 http://dx.doi.org/10.1371/journal.pone.0225876 Text en © 2019 Kamal et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kamal, Hira
Minhas, Fayyaz-ul-Amir Afsar
Tripathi, Diwaker
Abbasi, Wajid Arshad
Hamza, Muhammad
Mustafa, Roma
Khan, Muhammad Zuhaib
Mansoor, Shahid
Pappu, Hanu R.
Amin, Imran
βC1, pathogenicity determinant encoded by Cotton leaf curl Multan betasatellite, interacts with calmodulin-like protein 11 (Gh-CML11) in Gossypium hirsutum
title βC1, pathogenicity determinant encoded by Cotton leaf curl Multan betasatellite, interacts with calmodulin-like protein 11 (Gh-CML11) in Gossypium hirsutum
title_full βC1, pathogenicity determinant encoded by Cotton leaf curl Multan betasatellite, interacts with calmodulin-like protein 11 (Gh-CML11) in Gossypium hirsutum
title_fullStr βC1, pathogenicity determinant encoded by Cotton leaf curl Multan betasatellite, interacts with calmodulin-like protein 11 (Gh-CML11) in Gossypium hirsutum
title_full_unstemmed βC1, pathogenicity determinant encoded by Cotton leaf curl Multan betasatellite, interacts with calmodulin-like protein 11 (Gh-CML11) in Gossypium hirsutum
title_short βC1, pathogenicity determinant encoded by Cotton leaf curl Multan betasatellite, interacts with calmodulin-like protein 11 (Gh-CML11) in Gossypium hirsutum
title_sort βc1, pathogenicity determinant encoded by cotton leaf curl multan betasatellite, interacts with calmodulin-like protein 11 (gh-cml11) in gossypium hirsutum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890265/
https://www.ncbi.nlm.nih.gov/pubmed/31794580
http://dx.doi.org/10.1371/journal.pone.0225876
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