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Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism

Living cells proliferate by completing and coordinating two cycles, a division cycle controlling cell size and a DNA replication cycle controlling the number of chromosomal copies. It remains unclear how bacteria such as Escherichia coli tightly coordinate those two cycles across a wide range of gro...

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Detalles Bibliográficos
Autores principales: Witz, Guillaume, van Nimwegen, Erik, Julou, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890467/
https://www.ncbi.nlm.nih.gov/pubmed/31710292
http://dx.doi.org/10.7554/eLife.48063
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author Witz, Guillaume
van Nimwegen, Erik
Julou, Thomas
author_facet Witz, Guillaume
van Nimwegen, Erik
Julou, Thomas
author_sort Witz, Guillaume
collection PubMed
description Living cells proliferate by completing and coordinating two cycles, a division cycle controlling cell size and a DNA replication cycle controlling the number of chromosomal copies. It remains unclear how bacteria such as Escherichia coli tightly coordinate those two cycles across a wide range of growth conditions. Here, we used time-lapse microscopy in combination with microfluidics to measure growth, division and replication in single E. coli cells in both slow and fast growth conditions. To compare different phenomenological cell cycle models, we introduce a statistical framework assessing their ability to capture the correlation structure observed in the data. In combination with stochastic simulations, our data indicate that the cell cycle is driven from one initiation event to the next rather than from birth to division and is controlled by two adder mechanisms: the added volume since the last initiation event determines the timing of both the next division and replication initiation events.
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spelling pubmed-68904672019-12-06 Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism Witz, Guillaume van Nimwegen, Erik Julou, Thomas eLife Computational and Systems Biology Living cells proliferate by completing and coordinating two cycles, a division cycle controlling cell size and a DNA replication cycle controlling the number of chromosomal copies. It remains unclear how bacteria such as Escherichia coli tightly coordinate those two cycles across a wide range of growth conditions. Here, we used time-lapse microscopy in combination with microfluidics to measure growth, division and replication in single E. coli cells in both slow and fast growth conditions. To compare different phenomenological cell cycle models, we introduce a statistical framework assessing their ability to capture the correlation structure observed in the data. In combination with stochastic simulations, our data indicate that the cell cycle is driven from one initiation event to the next rather than from birth to division and is controlled by two adder mechanisms: the added volume since the last initiation event determines the timing of both the next division and replication initiation events. eLife Sciences Publications, Ltd 2019-11-11 /pmc/articles/PMC6890467/ /pubmed/31710292 http://dx.doi.org/10.7554/eLife.48063 Text en © 2019, Witz et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Computational and Systems Biology
Witz, Guillaume
van Nimwegen, Erik
Julou, Thomas
Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism
title Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism
title_full Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism
title_fullStr Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism
title_full_unstemmed Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism
title_short Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism
title_sort initiation of chromosome replication controls both division and replication cycles in e. coli through a double-adder mechanism
topic Computational and Systems Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890467/
https://www.ncbi.nlm.nih.gov/pubmed/31710292
http://dx.doi.org/10.7554/eLife.48063
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