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Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism
Living cells proliferate by completing and coordinating two cycles, a division cycle controlling cell size and a DNA replication cycle controlling the number of chromosomal copies. It remains unclear how bacteria such as Escherichia coli tightly coordinate those two cycles across a wide range of gro...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890467/ https://www.ncbi.nlm.nih.gov/pubmed/31710292 http://dx.doi.org/10.7554/eLife.48063 |
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author | Witz, Guillaume van Nimwegen, Erik Julou, Thomas |
author_facet | Witz, Guillaume van Nimwegen, Erik Julou, Thomas |
author_sort | Witz, Guillaume |
collection | PubMed |
description | Living cells proliferate by completing and coordinating two cycles, a division cycle controlling cell size and a DNA replication cycle controlling the number of chromosomal copies. It remains unclear how bacteria such as Escherichia coli tightly coordinate those two cycles across a wide range of growth conditions. Here, we used time-lapse microscopy in combination with microfluidics to measure growth, division and replication in single E. coli cells in both slow and fast growth conditions. To compare different phenomenological cell cycle models, we introduce a statistical framework assessing their ability to capture the correlation structure observed in the data. In combination with stochastic simulations, our data indicate that the cell cycle is driven from one initiation event to the next rather than from birth to division and is controlled by two adder mechanisms: the added volume since the last initiation event determines the timing of both the next division and replication initiation events. |
format | Online Article Text |
id | pubmed-6890467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-68904672019-12-06 Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism Witz, Guillaume van Nimwegen, Erik Julou, Thomas eLife Computational and Systems Biology Living cells proliferate by completing and coordinating two cycles, a division cycle controlling cell size and a DNA replication cycle controlling the number of chromosomal copies. It remains unclear how bacteria such as Escherichia coli tightly coordinate those two cycles across a wide range of growth conditions. Here, we used time-lapse microscopy in combination with microfluidics to measure growth, division and replication in single E. coli cells in both slow and fast growth conditions. To compare different phenomenological cell cycle models, we introduce a statistical framework assessing their ability to capture the correlation structure observed in the data. In combination with stochastic simulations, our data indicate that the cell cycle is driven from one initiation event to the next rather than from birth to division and is controlled by two adder mechanisms: the added volume since the last initiation event determines the timing of both the next division and replication initiation events. eLife Sciences Publications, Ltd 2019-11-11 /pmc/articles/PMC6890467/ /pubmed/31710292 http://dx.doi.org/10.7554/eLife.48063 Text en © 2019, Witz et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Computational and Systems Biology Witz, Guillaume van Nimwegen, Erik Julou, Thomas Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism |
title | Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism |
title_full | Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism |
title_fullStr | Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism |
title_full_unstemmed | Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism |
title_short | Initiation of chromosome replication controls both division and replication cycles in E. coli through a double-adder mechanism |
title_sort | initiation of chromosome replication controls both division and replication cycles in e. coli through a double-adder mechanism |
topic | Computational and Systems Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890467/ https://www.ncbi.nlm.nih.gov/pubmed/31710292 http://dx.doi.org/10.7554/eLife.48063 |
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