A new bioluminescence-based tool for modulating target proteins in live cells

We have developed a new genetically encoded tool designed to generate reactive oxygen species (ROS) at target proteins in cultured cells; it is designed using firefly luciferase and photosensitiser protein KillerRed. Targeting this fusion protein, KillerFirefly, to F-actin in live cells and treatmen...

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Autores principales: Ishimoto, Tetsuya, Mori, Hisashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890795/
https://www.ncbi.nlm.nih.gov/pubmed/31796796
http://dx.doi.org/10.1038/s41598-019-54712-y
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author Ishimoto, Tetsuya
Mori, Hisashi
author_facet Ishimoto, Tetsuya
Mori, Hisashi
author_sort Ishimoto, Tetsuya
collection PubMed
description We have developed a new genetically encoded tool designed to generate reactive oxygen species (ROS) at target proteins in cultured cells; it is designed using firefly luciferase and photosensitiser protein KillerRed. Targeting this fusion protein, KillerFirefly, to F-actin in live cells and treatment with luciferin induced a characteristic structure, previously reported as a cofilin-actin rod, which is seen in patients with Alzheimer’s disease. This structural change is considered to be elicited by the consistent generation of very low-level ROS by KillerFirefly in the vicinity of F-actin. Moreover, our results suggest the presence of an actin-regulating system, controlled by very low levels of endogenously generated ROS.
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spelling pubmed-68907952019-12-10 A new bioluminescence-based tool for modulating target proteins in live cells Ishimoto, Tetsuya Mori, Hisashi Sci Rep Article We have developed a new genetically encoded tool designed to generate reactive oxygen species (ROS) at target proteins in cultured cells; it is designed using firefly luciferase and photosensitiser protein KillerRed. Targeting this fusion protein, KillerFirefly, to F-actin in live cells and treatment with luciferin induced a characteristic structure, previously reported as a cofilin-actin rod, which is seen in patients with Alzheimer’s disease. This structural change is considered to be elicited by the consistent generation of very low-level ROS by KillerFirefly in the vicinity of F-actin. Moreover, our results suggest the presence of an actin-regulating system, controlled by very low levels of endogenously generated ROS. Nature Publishing Group UK 2019-12-03 /pmc/articles/PMC6890795/ /pubmed/31796796 http://dx.doi.org/10.1038/s41598-019-54712-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ishimoto, Tetsuya
Mori, Hisashi
A new bioluminescence-based tool for modulating target proteins in live cells
title A new bioluminescence-based tool for modulating target proteins in live cells
title_full A new bioluminescence-based tool for modulating target proteins in live cells
title_fullStr A new bioluminescence-based tool for modulating target proteins in live cells
title_full_unstemmed A new bioluminescence-based tool for modulating target proteins in live cells
title_short A new bioluminescence-based tool for modulating target proteins in live cells
title_sort new bioluminescence-based tool for modulating target proteins in live cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6890795/
https://www.ncbi.nlm.nih.gov/pubmed/31796796
http://dx.doi.org/10.1038/s41598-019-54712-y
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