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Retrochalcone Echinatin Triggers Apoptosis of Esophageal Squamous Cell Carcinoma via ROS- and ER Stress-Mediated Signaling Pathways

Esophageal squamous cell carcinoma (ESCC) is a poor prognostic cancer with a low five-year survival rate. Echinatin (Ech) is a retrochalone from licorice. It has been used as a herbal medicine due to its anti-inflammatory and anti-oxidative effects. However, its anticancer activity or underlying mec...

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Autores principales: Kwak, Ah-Won, Choi, Joon-Seok, Lee, Mee-Hyun, Oh, Ha-Na, Cho, Seung-Sik, Yoon, Goo, Liu, Kangdong, Chae, Jung-Il, Shim, Jung-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891341/
https://www.ncbi.nlm.nih.gov/pubmed/31717502
http://dx.doi.org/10.3390/molecules24224055
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author Kwak, Ah-Won
Choi, Joon-Seok
Lee, Mee-Hyun
Oh, Ha-Na
Cho, Seung-Sik
Yoon, Goo
Liu, Kangdong
Chae, Jung-Il
Shim, Jung-Hyun
author_facet Kwak, Ah-Won
Choi, Joon-Seok
Lee, Mee-Hyun
Oh, Ha-Na
Cho, Seung-Sik
Yoon, Goo
Liu, Kangdong
Chae, Jung-Il
Shim, Jung-Hyun
author_sort Kwak, Ah-Won
collection PubMed
description Esophageal squamous cell carcinoma (ESCC) is a poor prognostic cancer with a low five-year survival rate. Echinatin (Ech) is a retrochalone from licorice. It has been used as a herbal medicine due to its anti-inflammatory and anti-oxidative effects. However, its anticancer activity or underlying mechanism has not been elucidated yet. Thus, the objective of this study was to investigate the anti-tumor activity of Ech on ESCC by inducing ROS and ER stress dependent apoptosis. Ech inhibited ESCC cell growth in anchorage-dependent and independent analysis. Treatment with Ech induced G2/M phase of cell cycle and apoptosis of ESCC cells. It also regulated their related protein markers including p21, p27, cyclin B1, and cdc2. Ech also led to phosphorylation of JNK and p38. Regarding ROS and ER stress formation associated with apoptosis, we found that Ech increased ROS production, whereas its increase was diminished by NAC treatment. In addition, ER stress proteins were induced by treatment with Ech. Moreover, Ech enhanced MMP dysfunction and caspases activity. Furthermore, it regulated related biomarkers. Taken together, our results suggest that Ech can induce apoptosis in human ESCC cells via ROS/ER stress generation and p38 MAPK/JNK activation.
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spelling pubmed-68913412019-12-12 Retrochalcone Echinatin Triggers Apoptosis of Esophageal Squamous Cell Carcinoma via ROS- and ER Stress-Mediated Signaling Pathways Kwak, Ah-Won Choi, Joon-Seok Lee, Mee-Hyun Oh, Ha-Na Cho, Seung-Sik Yoon, Goo Liu, Kangdong Chae, Jung-Il Shim, Jung-Hyun Molecules Article Esophageal squamous cell carcinoma (ESCC) is a poor prognostic cancer with a low five-year survival rate. Echinatin (Ech) is a retrochalone from licorice. It has been used as a herbal medicine due to its anti-inflammatory and anti-oxidative effects. However, its anticancer activity or underlying mechanism has not been elucidated yet. Thus, the objective of this study was to investigate the anti-tumor activity of Ech on ESCC by inducing ROS and ER stress dependent apoptosis. Ech inhibited ESCC cell growth in anchorage-dependent and independent analysis. Treatment with Ech induced G2/M phase of cell cycle and apoptosis of ESCC cells. It also regulated their related protein markers including p21, p27, cyclin B1, and cdc2. Ech also led to phosphorylation of JNK and p38. Regarding ROS and ER stress formation associated with apoptosis, we found that Ech increased ROS production, whereas its increase was diminished by NAC treatment. In addition, ER stress proteins were induced by treatment with Ech. Moreover, Ech enhanced MMP dysfunction and caspases activity. Furthermore, it regulated related biomarkers. Taken together, our results suggest that Ech can induce apoptosis in human ESCC cells via ROS/ER stress generation and p38 MAPK/JNK activation. MDPI 2019-11-09 /pmc/articles/PMC6891341/ /pubmed/31717502 http://dx.doi.org/10.3390/molecules24224055 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kwak, Ah-Won
Choi, Joon-Seok
Lee, Mee-Hyun
Oh, Ha-Na
Cho, Seung-Sik
Yoon, Goo
Liu, Kangdong
Chae, Jung-Il
Shim, Jung-Hyun
Retrochalcone Echinatin Triggers Apoptosis of Esophageal Squamous Cell Carcinoma via ROS- and ER Stress-Mediated Signaling Pathways
title Retrochalcone Echinatin Triggers Apoptosis of Esophageal Squamous Cell Carcinoma via ROS- and ER Stress-Mediated Signaling Pathways
title_full Retrochalcone Echinatin Triggers Apoptosis of Esophageal Squamous Cell Carcinoma via ROS- and ER Stress-Mediated Signaling Pathways
title_fullStr Retrochalcone Echinatin Triggers Apoptosis of Esophageal Squamous Cell Carcinoma via ROS- and ER Stress-Mediated Signaling Pathways
title_full_unstemmed Retrochalcone Echinatin Triggers Apoptosis of Esophageal Squamous Cell Carcinoma via ROS- and ER Stress-Mediated Signaling Pathways
title_short Retrochalcone Echinatin Triggers Apoptosis of Esophageal Squamous Cell Carcinoma via ROS- and ER Stress-Mediated Signaling Pathways
title_sort retrochalcone echinatin triggers apoptosis of esophageal squamous cell carcinoma via ros- and er stress-mediated signaling pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891341/
https://www.ncbi.nlm.nih.gov/pubmed/31717502
http://dx.doi.org/10.3390/molecules24224055
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