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The Enhanced Sensitivity of a Porous Silicon Microcavity Biosensor Based on an Angular Spectrum Using CdSe/ZnS Quantum Dots
To improve the detection sensitivity of porous silicon microcavity biosensors, CdSe/ZnS quantum dots are used to label complementary DNA molecules for the refractive index amplification and angular spectrum method for detection. In this method, the TE mode laser is used as the detection light and th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891354/ https://www.ncbi.nlm.nih.gov/pubmed/31717344 http://dx.doi.org/10.3390/s19224872 |
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author | Zhou, Rui Jia, Zhenhong Lv, Xiaoyi Huang, Xiaohui |
author_facet | Zhou, Rui Jia, Zhenhong Lv, Xiaoyi Huang, Xiaohui |
author_sort | Zhou, Rui |
collection | PubMed |
description | To improve the detection sensitivity of porous silicon microcavity biosensors, CdSe/ZnS quantum dots are used to label complementary DNA molecules for the refractive index amplification and angular spectrum method for detection. In this method, the TE mode laser is used as the detection light and the light source is changed into a parallel beam by collimating and expanding the beam, which illuminates the PSM surface and receives the reflected light from the PSM surface through the detector. The angle corresponding to the weakest reflected light intensity before and after the biological reaction between probe DNA and complementary DNA of different concentrations labeled by quantum dots was measured by the detector, and the relationship between the angle change before and after the biological reaction and the complementary DNA concentration labeled by quantum dots was obtained. The experimental results show that the angle change increases linearly with increasing complementary DNA concentration. The detection limit of the experiment, as determined by fitting, is approximately 36 pM. The detection limit of this method is approximately 1/300 of that without quantum dot labeling. Our method has a low cost because it does not require the use of a reflectance spectrometer, and it also demonstrates high sensitivity. |
format | Online Article Text |
id | pubmed-6891354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68913542019-12-12 The Enhanced Sensitivity of a Porous Silicon Microcavity Biosensor Based on an Angular Spectrum Using CdSe/ZnS Quantum Dots Zhou, Rui Jia, Zhenhong Lv, Xiaoyi Huang, Xiaohui Sensors (Basel) Article To improve the detection sensitivity of porous silicon microcavity biosensors, CdSe/ZnS quantum dots are used to label complementary DNA molecules for the refractive index amplification and angular spectrum method for detection. In this method, the TE mode laser is used as the detection light and the light source is changed into a parallel beam by collimating and expanding the beam, which illuminates the PSM surface and receives the reflected light from the PSM surface through the detector. The angle corresponding to the weakest reflected light intensity before and after the biological reaction between probe DNA and complementary DNA of different concentrations labeled by quantum dots was measured by the detector, and the relationship between the angle change before and after the biological reaction and the complementary DNA concentration labeled by quantum dots was obtained. The experimental results show that the angle change increases linearly with increasing complementary DNA concentration. The detection limit of the experiment, as determined by fitting, is approximately 36 pM. The detection limit of this method is approximately 1/300 of that without quantum dot labeling. Our method has a low cost because it does not require the use of a reflectance spectrometer, and it also demonstrates high sensitivity. MDPI 2019-11-08 /pmc/articles/PMC6891354/ /pubmed/31717344 http://dx.doi.org/10.3390/s19224872 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhou, Rui Jia, Zhenhong Lv, Xiaoyi Huang, Xiaohui The Enhanced Sensitivity of a Porous Silicon Microcavity Biosensor Based on an Angular Spectrum Using CdSe/ZnS Quantum Dots |
title | The Enhanced Sensitivity of a Porous Silicon Microcavity Biosensor Based on an Angular Spectrum Using CdSe/ZnS Quantum Dots |
title_full | The Enhanced Sensitivity of a Porous Silicon Microcavity Biosensor Based on an Angular Spectrum Using CdSe/ZnS Quantum Dots |
title_fullStr | The Enhanced Sensitivity of a Porous Silicon Microcavity Biosensor Based on an Angular Spectrum Using CdSe/ZnS Quantum Dots |
title_full_unstemmed | The Enhanced Sensitivity of a Porous Silicon Microcavity Biosensor Based on an Angular Spectrum Using CdSe/ZnS Quantum Dots |
title_short | The Enhanced Sensitivity of a Porous Silicon Microcavity Biosensor Based on an Angular Spectrum Using CdSe/ZnS Quantum Dots |
title_sort | enhanced sensitivity of a porous silicon microcavity biosensor based on an angular spectrum using cdse/zns quantum dots |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891354/ https://www.ncbi.nlm.nih.gov/pubmed/31717344 http://dx.doi.org/10.3390/s19224872 |
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