Cargando…

Discrimination of the Geographical Origin of the Lateral Roots of Aconitum carmichaelii Using the Fingerprint, Multicomponent Quantification, and Chemometric Methods

Fuzi is a well-known traditional Chinese medicine developed from the lateral roots of Aconitum carmichaelii Debx. It is rich in alkaloids that display a wide variety of bioactivities, and it has a strong cardiotoxicity and neurotoxicity. In order to discriminate the geographical origin and evaluate...

Descripción completa

Detalles Bibliográficos
Autores principales: Miao, Lu-Lin, Zhou, Qin-Mei, Peng, Cheng, Meng, Chun-Wang, Wang, Xiao-Ya, Xiong, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891363/
https://www.ncbi.nlm.nih.gov/pubmed/31739601
http://dx.doi.org/10.3390/molecules24224124
_version_ 1783475796037337088
author Miao, Lu-Lin
Zhou, Qin-Mei
Peng, Cheng
Meng, Chun-Wang
Wang, Xiao-Ya
Xiong, Liang
author_facet Miao, Lu-Lin
Zhou, Qin-Mei
Peng, Cheng
Meng, Chun-Wang
Wang, Xiao-Ya
Xiong, Liang
author_sort Miao, Lu-Lin
collection PubMed
description Fuzi is a well-known traditional Chinese medicine developed from the lateral roots of Aconitum carmichaelii Debx. It is rich in alkaloids that display a wide variety of bioactivities, and it has a strong cardiotoxicity and neurotoxicity. In order to discriminate the geographical origin and evaluate the quality of this medicine, a method based on high-performance liquid chromatography (HPLC) was developed for multicomponent quantification and chemical fingerprint analysis. The measured results of 32 batches of Fuzi from three different regions were evaluated by chemometric analysis, including similarity analysis (SA), hierarchical cluster analysis (HCA), principal component analysis (PCA), and linear discriminant analysis (LDA). The content of six representative alkaloids of Fuzi (benzoylmesaconine, benzoylhypaconine, benzoylaconine, mesaconitine, hypaconitine, and aconitine) were varied by geographical origin, and the content ratios of the benzoylmesaconine/mesaconitine and diester-type/monoester-type diterpenoid alkaloids may be potential traits for classifying the geographical origin of the medicine. In the HPLC fingerprint similarity analysis, the Fuzi from Jiangyou, Sichuan, was distinguished from the Fuzi from Butuo, Sichuan, and the Fuzi from Yunnan. Based on the HCA and PCA analyses of the content of the six representative alkaloids, all of the batches were classified into two categories, which were closely related to the plants’ geographical origins. The Fuzi samples from Jiangyou were placed into one category, while the Fuzi samples from Butuo and Yunnan were put into another category. The LDA analysis provided an efficient and satisfactory prediction model for differentiating the Fuzi samples from the above-mentioned three geographical origins. Thus, the content of the six representative alkaloids and the fingerprint similarity values were useful markers for differentiating the geographical origin of the Fuzi samples.
format Online
Article
Text
id pubmed-6891363
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68913632019-12-12 Discrimination of the Geographical Origin of the Lateral Roots of Aconitum carmichaelii Using the Fingerprint, Multicomponent Quantification, and Chemometric Methods Miao, Lu-Lin Zhou, Qin-Mei Peng, Cheng Meng, Chun-Wang Wang, Xiao-Ya Xiong, Liang Molecules Article Fuzi is a well-known traditional Chinese medicine developed from the lateral roots of Aconitum carmichaelii Debx. It is rich in alkaloids that display a wide variety of bioactivities, and it has a strong cardiotoxicity and neurotoxicity. In order to discriminate the geographical origin and evaluate the quality of this medicine, a method based on high-performance liquid chromatography (HPLC) was developed for multicomponent quantification and chemical fingerprint analysis. The measured results of 32 batches of Fuzi from three different regions were evaluated by chemometric analysis, including similarity analysis (SA), hierarchical cluster analysis (HCA), principal component analysis (PCA), and linear discriminant analysis (LDA). The content of six representative alkaloids of Fuzi (benzoylmesaconine, benzoylhypaconine, benzoylaconine, mesaconitine, hypaconitine, and aconitine) were varied by geographical origin, and the content ratios of the benzoylmesaconine/mesaconitine and diester-type/monoester-type diterpenoid alkaloids may be potential traits for classifying the geographical origin of the medicine. In the HPLC fingerprint similarity analysis, the Fuzi from Jiangyou, Sichuan, was distinguished from the Fuzi from Butuo, Sichuan, and the Fuzi from Yunnan. Based on the HCA and PCA analyses of the content of the six representative alkaloids, all of the batches were classified into two categories, which were closely related to the plants’ geographical origins. The Fuzi samples from Jiangyou were placed into one category, while the Fuzi samples from Butuo and Yunnan were put into another category. The LDA analysis provided an efficient and satisfactory prediction model for differentiating the Fuzi samples from the above-mentioned three geographical origins. Thus, the content of the six representative alkaloids and the fingerprint similarity values were useful markers for differentiating the geographical origin of the Fuzi samples. MDPI 2019-11-14 /pmc/articles/PMC6891363/ /pubmed/31739601 http://dx.doi.org/10.3390/molecules24224124 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Miao, Lu-Lin
Zhou, Qin-Mei
Peng, Cheng
Meng, Chun-Wang
Wang, Xiao-Ya
Xiong, Liang
Discrimination of the Geographical Origin of the Lateral Roots of Aconitum carmichaelii Using the Fingerprint, Multicomponent Quantification, and Chemometric Methods
title Discrimination of the Geographical Origin of the Lateral Roots of Aconitum carmichaelii Using the Fingerprint, Multicomponent Quantification, and Chemometric Methods
title_full Discrimination of the Geographical Origin of the Lateral Roots of Aconitum carmichaelii Using the Fingerprint, Multicomponent Quantification, and Chemometric Methods
title_fullStr Discrimination of the Geographical Origin of the Lateral Roots of Aconitum carmichaelii Using the Fingerprint, Multicomponent Quantification, and Chemometric Methods
title_full_unstemmed Discrimination of the Geographical Origin of the Lateral Roots of Aconitum carmichaelii Using the Fingerprint, Multicomponent Quantification, and Chemometric Methods
title_short Discrimination of the Geographical Origin of the Lateral Roots of Aconitum carmichaelii Using the Fingerprint, Multicomponent Quantification, and Chemometric Methods
title_sort discrimination of the geographical origin of the lateral roots of aconitum carmichaelii using the fingerprint, multicomponent quantification, and chemometric methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891363/
https://www.ncbi.nlm.nih.gov/pubmed/31739601
http://dx.doi.org/10.3390/molecules24224124
work_keys_str_mv AT miaolulin discriminationofthegeographicaloriginofthelateralrootsofaconitumcarmichaeliiusingthefingerprintmulticomponentquantificationandchemometricmethods
AT zhouqinmei discriminationofthegeographicaloriginofthelateralrootsofaconitumcarmichaeliiusingthefingerprintmulticomponentquantificationandchemometricmethods
AT pengcheng discriminationofthegeographicaloriginofthelateralrootsofaconitumcarmichaeliiusingthefingerprintmulticomponentquantificationandchemometricmethods
AT mengchunwang discriminationofthegeographicaloriginofthelateralrootsofaconitumcarmichaeliiusingthefingerprintmulticomponentquantificationandchemometricmethods
AT wangxiaoya discriminationofthegeographicaloriginofthelateralrootsofaconitumcarmichaeliiusingthefingerprintmulticomponentquantificationandchemometricmethods
AT xiongliang discriminationofthegeographicaloriginofthelateralrootsofaconitumcarmichaeliiusingthefingerprintmulticomponentquantificationandchemometricmethods