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Analysis of Multiclass Antibiotics in Lettuce by Liquid Chromatography–Tandem Mass Spectrometry to Monitor Their Plant Uptake

The main entry routes of antibiotics in the environment are the application of organic wastes to improve soil quality and the irrigation with recycled water. Once in the environment, antibiotics can be introduced in the food chain through their uptake by crops. This paper describes the development o...

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Autores principales: Albero, Beatriz, Tadeo, José L., Delgado, María del Mar, Miguel, Esther, Pérez, Rosa Ana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891457/
https://www.ncbi.nlm.nih.gov/pubmed/31717615
http://dx.doi.org/10.3390/molecules24224066
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author Albero, Beatriz
Tadeo, José L.
Delgado, María del Mar
Miguel, Esther
Pérez, Rosa Ana
author_facet Albero, Beatriz
Tadeo, José L.
Delgado, María del Mar
Miguel, Esther
Pérez, Rosa Ana
author_sort Albero, Beatriz
collection PubMed
description The main entry routes of antibiotics in the environment are the application of organic wastes to improve soil quality and the irrigation with recycled water. Once in the environment, antibiotics can be introduced in the food chain through their uptake by crops. This paper describes the development of an analytical method based on ultrasound-assisted extraction for the determination of seven antibiotics in lettuce. The developed method was applied to evaluate antibiotic uptake by lettuce grown in pots fertilized with composted poultry litter doped with a mixture of antibiotics to reach a final concentration of 2.5 µg/g in soil. Lettuce were harvested after 21, 36, and 55 days. Five of the seven studied antibiotics were found in all samples. The highest uptake was found for lincomycin (51 ng/g fresh weight) followed by sulfamethoxazole (44 ng/g fresh weight) and sulfamethazine (21 ng/g fresh weight) in lettuce harvested after 21 days. An important decrease of their levels was observed after 36 days, but these levels remained similar after 55 days. Although levels found in lettuce were low, the presence of antibiotics demonstrates the need for further assessing food safety risks related with the use of soil amendments or irrigation water contaminated with antibiotics.
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spelling pubmed-68914572019-12-18 Analysis of Multiclass Antibiotics in Lettuce by Liquid Chromatography–Tandem Mass Spectrometry to Monitor Their Plant Uptake Albero, Beatriz Tadeo, José L. Delgado, María del Mar Miguel, Esther Pérez, Rosa Ana Molecules Article The main entry routes of antibiotics in the environment are the application of organic wastes to improve soil quality and the irrigation with recycled water. Once in the environment, antibiotics can be introduced in the food chain through their uptake by crops. This paper describes the development of an analytical method based on ultrasound-assisted extraction for the determination of seven antibiotics in lettuce. The developed method was applied to evaluate antibiotic uptake by lettuce grown in pots fertilized with composted poultry litter doped with a mixture of antibiotics to reach a final concentration of 2.5 µg/g in soil. Lettuce were harvested after 21, 36, and 55 days. Five of the seven studied antibiotics were found in all samples. The highest uptake was found for lincomycin (51 ng/g fresh weight) followed by sulfamethoxazole (44 ng/g fresh weight) and sulfamethazine (21 ng/g fresh weight) in lettuce harvested after 21 days. An important decrease of their levels was observed after 36 days, but these levels remained similar after 55 days. Although levels found in lettuce were low, the presence of antibiotics demonstrates the need for further assessing food safety risks related with the use of soil amendments or irrigation water contaminated with antibiotics. MDPI 2019-11-10 /pmc/articles/PMC6891457/ /pubmed/31717615 http://dx.doi.org/10.3390/molecules24224066 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Albero, Beatriz
Tadeo, José L.
Delgado, María del Mar
Miguel, Esther
Pérez, Rosa Ana
Analysis of Multiclass Antibiotics in Lettuce by Liquid Chromatography–Tandem Mass Spectrometry to Monitor Their Plant Uptake
title Analysis of Multiclass Antibiotics in Lettuce by Liquid Chromatography–Tandem Mass Spectrometry to Monitor Their Plant Uptake
title_full Analysis of Multiclass Antibiotics in Lettuce by Liquid Chromatography–Tandem Mass Spectrometry to Monitor Their Plant Uptake
title_fullStr Analysis of Multiclass Antibiotics in Lettuce by Liquid Chromatography–Tandem Mass Spectrometry to Monitor Their Plant Uptake
title_full_unstemmed Analysis of Multiclass Antibiotics in Lettuce by Liquid Chromatography–Tandem Mass Spectrometry to Monitor Their Plant Uptake
title_short Analysis of Multiclass Antibiotics in Lettuce by Liquid Chromatography–Tandem Mass Spectrometry to Monitor Their Plant Uptake
title_sort analysis of multiclass antibiotics in lettuce by liquid chromatography–tandem mass spectrometry to monitor their plant uptake
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891457/
https://www.ncbi.nlm.nih.gov/pubmed/31717615
http://dx.doi.org/10.3390/molecules24224066
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