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Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets

The Fusarium mycotoxin deoxynivalenol (DON) contaminates animal feed worldwide. In vivo, DON modifies the cellular protein synthesis, thereby also affecting the immune system. However, the functional consequences of this are still ill-defined. In this study, peripheral blood mononuclear cells from h...

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Autores principales: Vatzia, Eleni, Pierron, Alix, Saalmüller, Armin, Mayer, Elisabeth, Gerner, Wilhelm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891462/
https://www.ncbi.nlm.nih.gov/pubmed/31694331
http://dx.doi.org/10.3390/toxins11110644
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author Vatzia, Eleni
Pierron, Alix
Saalmüller, Armin
Mayer, Elisabeth
Gerner, Wilhelm
author_facet Vatzia, Eleni
Pierron, Alix
Saalmüller, Armin
Mayer, Elisabeth
Gerner, Wilhelm
author_sort Vatzia, Eleni
collection PubMed
description The Fusarium mycotoxin deoxynivalenol (DON) contaminates animal feed worldwide. In vivo, DON modifies the cellular protein synthesis, thereby also affecting the immune system. However, the functional consequences of this are still ill-defined. In this study, peripheral blood mononuclear cells from healthy pigs were incubated with different DON concentrations in the presence of Concanavalin A (ConA), a plant-derived polyclonal T-cell stimulant. T-cell subsets were investigated for proliferation and expression of CD8α, CD27, and CD28, which are involved in activation and costimulation of porcine T cells. A clear decrease in proliferation of all ConA-stimulated major T-cell subsets (CD4(+), CD8(+), and γδ T cells) was observed in DON concentrations higher than 0.4 µM. This applied in particular to naïve CD4(+) and CD8(+) T cells. From 0.8 μM onwards, DON induced a reduction of CD8α (CD4(+)) and CD27 expression (CD4(+) and CD8(+) T cells). CD28 expression was diminished in CD4(+) and CD8(+) T cells at a concentration of 1.6 µM DON. None of these effects were observed with the DON-derivative deepoxy-deoxynivalenol (DOM-1) at 16 µM. These results indicate that DON reduces T-cell proliferation and the expression of molecules involved in T-cell activation, providing a molecular basis for some of the described immunosuppressive effects of DON.
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spelling pubmed-68914622019-12-18 Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets Vatzia, Eleni Pierron, Alix Saalmüller, Armin Mayer, Elisabeth Gerner, Wilhelm Toxins (Basel) Article The Fusarium mycotoxin deoxynivalenol (DON) contaminates animal feed worldwide. In vivo, DON modifies the cellular protein synthesis, thereby also affecting the immune system. However, the functional consequences of this are still ill-defined. In this study, peripheral blood mononuclear cells from healthy pigs were incubated with different DON concentrations in the presence of Concanavalin A (ConA), a plant-derived polyclonal T-cell stimulant. T-cell subsets were investigated for proliferation and expression of CD8α, CD27, and CD28, which are involved in activation and costimulation of porcine T cells. A clear decrease in proliferation of all ConA-stimulated major T-cell subsets (CD4(+), CD8(+), and γδ T cells) was observed in DON concentrations higher than 0.4 µM. This applied in particular to naïve CD4(+) and CD8(+) T cells. From 0.8 μM onwards, DON induced a reduction of CD8α (CD4(+)) and CD27 expression (CD4(+) and CD8(+) T cells). CD28 expression was diminished in CD4(+) and CD8(+) T cells at a concentration of 1.6 µM DON. None of these effects were observed with the DON-derivative deepoxy-deoxynivalenol (DOM-1) at 16 µM. These results indicate that DON reduces T-cell proliferation and the expression of molecules involved in T-cell activation, providing a molecular basis for some of the described immunosuppressive effects of DON. MDPI 2019-11-05 /pmc/articles/PMC6891462/ /pubmed/31694331 http://dx.doi.org/10.3390/toxins11110644 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vatzia, Eleni
Pierron, Alix
Saalmüller, Armin
Mayer, Elisabeth
Gerner, Wilhelm
Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets
title Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets
title_full Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets
title_fullStr Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets
title_full_unstemmed Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets
title_short Deoxynivalenol Affects Proliferation and Expression of Activation-Related Molecules in Major Porcine T-Cell Subsets
title_sort deoxynivalenol affects proliferation and expression of activation-related molecules in major porcine t-cell subsets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891462/
https://www.ncbi.nlm.nih.gov/pubmed/31694331
http://dx.doi.org/10.3390/toxins11110644
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