Cargando…
A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective
Fourier ptychography microscopy (FPM) is a recently emerged computational imaging method, which combines the advantages of synthetic aperture and phase retrieval to achieve super-resolution microscopic imaging. FPM can bypass the diffraction limit of the numerical aperture (NA) system and achieve co...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891469/ https://www.ncbi.nlm.nih.gov/pubmed/31717982 http://dx.doi.org/10.3390/s19224913 |
_version_ | 1783475821360447488 |
---|---|
author | Zhang, Shaohui Zhou, Guocheng Wang, Ying Hu, Yao Hao, Qun |
author_facet | Zhang, Shaohui Zhou, Guocheng Wang, Ying Hu, Yao Hao, Qun |
author_sort | Zhang, Shaohui |
collection | PubMed |
description | Fourier ptychography microscopy (FPM) is a recently emerged computational imaging method, which combines the advantages of synthetic aperture and phase retrieval to achieve super-resolution microscopic imaging. FPM can bypass the diffraction limit of the numerical aperture (NA) system and achieve complex images with wide field of view and high resolution (HR) on the basis of the existing microscopic platform, which has low resolution and wide field of view. Conventional FPM platforms are constructed based on basic microscopic platform and a scientific complementary metal–oxide–semiconductor (sCMOS) camera, which has ultrahigh dynamic range. However, sCMOS, or even the microscopic platform, is too expensive to afford for some researchers. Furthermore, the fixed microscopic platform limits the space for function expansion and system modification. In this work, we present a simply equipped FPM platform based on an industrial camera and telecentric objective, which is much cheaper than sCMOS camera and microscopic platform and has accurate optical calibration. A corresponding algorithm was embedded into a conventional FP framework to overcome the low dynamic range of industrial cameras. Simulation and experimental results showed the feasibility and good performance of the designed FPM platform and algorithms. |
format | Online Article Text |
id | pubmed-6891469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68914692019-12-18 A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective Zhang, Shaohui Zhou, Guocheng Wang, Ying Hu, Yao Hao, Qun Sensors (Basel) Article Fourier ptychography microscopy (FPM) is a recently emerged computational imaging method, which combines the advantages of synthetic aperture and phase retrieval to achieve super-resolution microscopic imaging. FPM can bypass the diffraction limit of the numerical aperture (NA) system and achieve complex images with wide field of view and high resolution (HR) on the basis of the existing microscopic platform, which has low resolution and wide field of view. Conventional FPM platforms are constructed based on basic microscopic platform and a scientific complementary metal–oxide–semiconductor (sCMOS) camera, which has ultrahigh dynamic range. However, sCMOS, or even the microscopic platform, is too expensive to afford for some researchers. Furthermore, the fixed microscopic platform limits the space for function expansion and system modification. In this work, we present a simply equipped FPM platform based on an industrial camera and telecentric objective, which is much cheaper than sCMOS camera and microscopic platform and has accurate optical calibration. A corresponding algorithm was embedded into a conventional FP framework to overcome the low dynamic range of industrial cameras. Simulation and experimental results showed the feasibility and good performance of the designed FPM platform and algorithms. MDPI 2019-11-11 /pmc/articles/PMC6891469/ /pubmed/31717982 http://dx.doi.org/10.3390/s19224913 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Shaohui Zhou, Guocheng Wang, Ying Hu, Yao Hao, Qun A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective |
title | A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective |
title_full | A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective |
title_fullStr | A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective |
title_full_unstemmed | A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective |
title_short | A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective |
title_sort | simply equipped fourier ptychography platform based on an industrial camera and telecentric objective |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891469/ https://www.ncbi.nlm.nih.gov/pubmed/31717982 http://dx.doi.org/10.3390/s19224913 |
work_keys_str_mv | AT zhangshaohui asimplyequippedfourierptychographyplatformbasedonanindustrialcameraandtelecentricobjective AT zhouguocheng asimplyequippedfourierptychographyplatformbasedonanindustrialcameraandtelecentricobjective AT wangying asimplyequippedfourierptychographyplatformbasedonanindustrialcameraandtelecentricobjective AT huyao asimplyequippedfourierptychographyplatformbasedonanindustrialcameraandtelecentricobjective AT haoqun asimplyequippedfourierptychographyplatformbasedonanindustrialcameraandtelecentricobjective AT zhangshaohui simplyequippedfourierptychographyplatformbasedonanindustrialcameraandtelecentricobjective AT zhouguocheng simplyequippedfourierptychographyplatformbasedonanindustrialcameraandtelecentricobjective AT wangying simplyequippedfourierptychographyplatformbasedonanindustrialcameraandtelecentricobjective AT huyao simplyequippedfourierptychographyplatformbasedonanindustrialcameraandtelecentricobjective AT haoqun simplyequippedfourierptychographyplatformbasedonanindustrialcameraandtelecentricobjective |