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A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective

Fourier ptychography microscopy (FPM) is a recently emerged computational imaging method, which combines the advantages of synthetic aperture and phase retrieval to achieve super-resolution microscopic imaging. FPM can bypass the diffraction limit of the numerical aperture (NA) system and achieve co...

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Detalles Bibliográficos
Autores principales: Zhang, Shaohui, Zhou, Guocheng, Wang, Ying, Hu, Yao, Hao, Qun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891469/
https://www.ncbi.nlm.nih.gov/pubmed/31717982
http://dx.doi.org/10.3390/s19224913
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author Zhang, Shaohui
Zhou, Guocheng
Wang, Ying
Hu, Yao
Hao, Qun
author_facet Zhang, Shaohui
Zhou, Guocheng
Wang, Ying
Hu, Yao
Hao, Qun
author_sort Zhang, Shaohui
collection PubMed
description Fourier ptychography microscopy (FPM) is a recently emerged computational imaging method, which combines the advantages of synthetic aperture and phase retrieval to achieve super-resolution microscopic imaging. FPM can bypass the diffraction limit of the numerical aperture (NA) system and achieve complex images with wide field of view and high resolution (HR) on the basis of the existing microscopic platform, which has low resolution and wide field of view. Conventional FPM platforms are constructed based on basic microscopic platform and a scientific complementary metal–oxide–semiconductor (sCMOS) camera, which has ultrahigh dynamic range. However, sCMOS, or even the microscopic platform, is too expensive to afford for some researchers. Furthermore, the fixed microscopic platform limits the space for function expansion and system modification. In this work, we present a simply equipped FPM platform based on an industrial camera and telecentric objective, which is much cheaper than sCMOS camera and microscopic platform and has accurate optical calibration. A corresponding algorithm was embedded into a conventional FP framework to overcome the low dynamic range of industrial cameras. Simulation and experimental results showed the feasibility and good performance of the designed FPM platform and algorithms.
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spelling pubmed-68914692019-12-18 A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective Zhang, Shaohui Zhou, Guocheng Wang, Ying Hu, Yao Hao, Qun Sensors (Basel) Article Fourier ptychography microscopy (FPM) is a recently emerged computational imaging method, which combines the advantages of synthetic aperture and phase retrieval to achieve super-resolution microscopic imaging. FPM can bypass the diffraction limit of the numerical aperture (NA) system and achieve complex images with wide field of view and high resolution (HR) on the basis of the existing microscopic platform, which has low resolution and wide field of view. Conventional FPM platforms are constructed based on basic microscopic platform and a scientific complementary metal–oxide–semiconductor (sCMOS) camera, which has ultrahigh dynamic range. However, sCMOS, or even the microscopic platform, is too expensive to afford for some researchers. Furthermore, the fixed microscopic platform limits the space for function expansion and system modification. In this work, we present a simply equipped FPM platform based on an industrial camera and telecentric objective, which is much cheaper than sCMOS camera and microscopic platform and has accurate optical calibration. A corresponding algorithm was embedded into a conventional FP framework to overcome the low dynamic range of industrial cameras. Simulation and experimental results showed the feasibility and good performance of the designed FPM platform and algorithms. MDPI 2019-11-11 /pmc/articles/PMC6891469/ /pubmed/31717982 http://dx.doi.org/10.3390/s19224913 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Shaohui
Zhou, Guocheng
Wang, Ying
Hu, Yao
Hao, Qun
A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective
title A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective
title_full A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective
title_fullStr A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective
title_full_unstemmed A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective
title_short A Simply Equipped Fourier Ptychography Platform Based on an Industrial Camera and Telecentric Objective
title_sort simply equipped fourier ptychography platform based on an industrial camera and telecentric objective
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891469/
https://www.ncbi.nlm.nih.gov/pubmed/31717982
http://dx.doi.org/10.3390/s19224913
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