Cargando…
Single Production of Kojic Acid by Aspergillus flavus and the Revision of Flufuran
Timor Island is very hot and dry due to the high intensity of sunlight experienced throughout the year. The endophytic fungi Aspergillus flavus had been isolated from medicinal plants such as Catharanthus roseus, Annona squamosa and Curcuma xanthorisa. The endophytic fungi A. flavus from each plant...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891502/ https://www.ncbi.nlm.nih.gov/pubmed/31752404 http://dx.doi.org/10.3390/molecules24224200 |
_version_ | 1783475829148221440 |
---|---|
author | Ola, Antonius R. B. Metboki, Gema Lay, Caterina S. Sugi, Yoseph Rozari, Philipi De Darmakusuma, Dodi Hakim, Euis Holisotan |
author_facet | Ola, Antonius R. B. Metboki, Gema Lay, Caterina S. Sugi, Yoseph Rozari, Philipi De Darmakusuma, Dodi Hakim, Euis Holisotan |
author_sort | Ola, Antonius R. B. |
collection | PubMed |
description | Timor Island is very hot and dry due to the high intensity of sunlight experienced throughout the year. The endophytic fungi Aspergillus flavus had been isolated from medicinal plants such as Catharanthus roseus, Annona squamosa and Curcuma xanthorisa. The endophytic fungi A. flavus from each plant was cultivated on solid rice media and then analyzed for its capability for producing kojic acid. The production of kojic acid was analyzed by HPLC; the highest amount of kojic acid was observed from the endophytic fungi A. flavus, isolated from the stem of Catharanthus roseus, followed by A. flavus from Annona squamosa and Curcuma xanthorisa. Simple VLC fractionation of the extract of A. flavus from C.roseus led to the isolation of around 11.1 g of pure kojic acid. The structure of kojic acid (1) was confirmed by NMR and MS spectroscopic data. A comparison of the NMR data with the literature supported the revision of the natural product flufuran to kojic acid. To the best of our knowledge, this is the first report of a strain of endophytic fungi producing only kojic acid without any other toxic metabolites such as alfatoxins. Therefore, this Aspergillus flavus strain can be applied as a potential producer of kojic acid for industrial use. |
format | Online Article Text |
id | pubmed-6891502 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68915022019-12-18 Single Production of Kojic Acid by Aspergillus flavus and the Revision of Flufuran Ola, Antonius R. B. Metboki, Gema Lay, Caterina S. Sugi, Yoseph Rozari, Philipi De Darmakusuma, Dodi Hakim, Euis Holisotan Molecules Communication Timor Island is very hot and dry due to the high intensity of sunlight experienced throughout the year. The endophytic fungi Aspergillus flavus had been isolated from medicinal plants such as Catharanthus roseus, Annona squamosa and Curcuma xanthorisa. The endophytic fungi A. flavus from each plant was cultivated on solid rice media and then analyzed for its capability for producing kojic acid. The production of kojic acid was analyzed by HPLC; the highest amount of kojic acid was observed from the endophytic fungi A. flavus, isolated from the stem of Catharanthus roseus, followed by A. flavus from Annona squamosa and Curcuma xanthorisa. Simple VLC fractionation of the extract of A. flavus from C.roseus led to the isolation of around 11.1 g of pure kojic acid. The structure of kojic acid (1) was confirmed by NMR and MS spectroscopic data. A comparison of the NMR data with the literature supported the revision of the natural product flufuran to kojic acid. To the best of our knowledge, this is the first report of a strain of endophytic fungi producing only kojic acid without any other toxic metabolites such as alfatoxins. Therefore, this Aspergillus flavus strain can be applied as a potential producer of kojic acid for industrial use. MDPI 2019-11-19 /pmc/articles/PMC6891502/ /pubmed/31752404 http://dx.doi.org/10.3390/molecules24224200 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Ola, Antonius R. B. Metboki, Gema Lay, Caterina S. Sugi, Yoseph Rozari, Philipi De Darmakusuma, Dodi Hakim, Euis Holisotan Single Production of Kojic Acid by Aspergillus flavus and the Revision of Flufuran |
title | Single Production of Kojic Acid by Aspergillus flavus and the Revision of Flufuran |
title_full | Single Production of Kojic Acid by Aspergillus flavus and the Revision of Flufuran |
title_fullStr | Single Production of Kojic Acid by Aspergillus flavus and the Revision of Flufuran |
title_full_unstemmed | Single Production of Kojic Acid by Aspergillus flavus and the Revision of Flufuran |
title_short | Single Production of Kojic Acid by Aspergillus flavus and the Revision of Flufuran |
title_sort | single production of kojic acid by aspergillus flavus and the revision of flufuran |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891502/ https://www.ncbi.nlm.nih.gov/pubmed/31752404 http://dx.doi.org/10.3390/molecules24224200 |
work_keys_str_mv | AT olaantoniusrb singleproductionofkojicacidbyaspergillusflavusandtherevisionofflufuran AT metbokigema singleproductionofkojicacidbyaspergillusflavusandtherevisionofflufuran AT laycaterinas singleproductionofkojicacidbyaspergillusflavusandtherevisionofflufuran AT sugiyoseph singleproductionofkojicacidbyaspergillusflavusandtherevisionofflufuran AT rozariphilipide singleproductionofkojicacidbyaspergillusflavusandtherevisionofflufuran AT darmakusumadodi singleproductionofkojicacidbyaspergillusflavusandtherevisionofflufuran AT hakimeuisholisotan singleproductionofkojicacidbyaspergillusflavusandtherevisionofflufuran |