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Sensitively and Selectively Detect Biothiols by Using Fluorescence Method and Resonance Light Scattering Technique Simultaneously
In this paper, we designed a new quantitative and qualitive detection method for biothiols by using fluorescence method and resonance light scattering (RLS) technique. Nitrogen doped carbon quantum dots (C/N-dots) were obtained from tartaric acid and ethylenediamine by hydrothermal method, and then...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891520/ https://www.ncbi.nlm.nih.gov/pubmed/31731646 http://dx.doi.org/10.3390/molecules24224136 |
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author | Shi, Yanping Sun, Chao Gao, Xiaoqi Zhao, Wei Zhou, Nan |
author_facet | Shi, Yanping Sun, Chao Gao, Xiaoqi Zhao, Wei Zhou, Nan |
author_sort | Shi, Yanping |
collection | PubMed |
description | In this paper, we designed a new quantitative and qualitive detection method for biothiols by using fluorescence method and resonance light scattering (RLS) technique. Nitrogen doped carbon quantum dots (C/N-dots) were obtained from tartaric acid and ethylenediamine by hydrothermal method, and then their morphology and optical properties were characterized by different techniques. A detection system consisting of C/N-dots and Ag(+) complex was established. In this system, C/N-dots possessed the photoluminescent property and the Ag(+) complex owned the RLS property, so, by combining the two luminescent properties to achieve complementary advantages, we could detect biothiols and solve the problem of distinguishing between Cys and GSH. Additionally, we optimized detecting conditions and investigated the detection mechanism of fluorescence quenching and RLS detecting. Results showed that the analytical response of fluorescence was linear in the range 0–140 μM and the detection limit (LOD) was calculated to be 6.6 μM for Cys, and the addition of GSH had no effect on fluorescence. RLS response ranges were 0–167 μM for Cys and 0–200 μM for GSH, with LOD down to 64 nM and 74 nM, respectively. Furthermore, the probe was successfully used for detecting Cys in fetal bovine serum (FBS) samples by fluorescence method, and also, by RLS technique, the content of GSH in FBS samples was detected. |
format | Online Article Text |
id | pubmed-6891520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68915202019-12-18 Sensitively and Selectively Detect Biothiols by Using Fluorescence Method and Resonance Light Scattering Technique Simultaneously Shi, Yanping Sun, Chao Gao, Xiaoqi Zhao, Wei Zhou, Nan Molecules Article In this paper, we designed a new quantitative and qualitive detection method for biothiols by using fluorescence method and resonance light scattering (RLS) technique. Nitrogen doped carbon quantum dots (C/N-dots) were obtained from tartaric acid and ethylenediamine by hydrothermal method, and then their morphology and optical properties were characterized by different techniques. A detection system consisting of C/N-dots and Ag(+) complex was established. In this system, C/N-dots possessed the photoluminescent property and the Ag(+) complex owned the RLS property, so, by combining the two luminescent properties to achieve complementary advantages, we could detect biothiols and solve the problem of distinguishing between Cys and GSH. Additionally, we optimized detecting conditions and investigated the detection mechanism of fluorescence quenching and RLS detecting. Results showed that the analytical response of fluorescence was linear in the range 0–140 μM and the detection limit (LOD) was calculated to be 6.6 μM for Cys, and the addition of GSH had no effect on fluorescence. RLS response ranges were 0–167 μM for Cys and 0–200 μM for GSH, with LOD down to 64 nM and 74 nM, respectively. Furthermore, the probe was successfully used for detecting Cys in fetal bovine serum (FBS) samples by fluorescence method, and also, by RLS technique, the content of GSH in FBS samples was detected. MDPI 2019-11-15 /pmc/articles/PMC6891520/ /pubmed/31731646 http://dx.doi.org/10.3390/molecules24224136 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shi, Yanping Sun, Chao Gao, Xiaoqi Zhao, Wei Zhou, Nan Sensitively and Selectively Detect Biothiols by Using Fluorescence Method and Resonance Light Scattering Technique Simultaneously |
title | Sensitively and Selectively Detect Biothiols by Using Fluorescence Method and Resonance Light Scattering Technique Simultaneously |
title_full | Sensitively and Selectively Detect Biothiols by Using Fluorescence Method and Resonance Light Scattering Technique Simultaneously |
title_fullStr | Sensitively and Selectively Detect Biothiols by Using Fluorescence Method and Resonance Light Scattering Technique Simultaneously |
title_full_unstemmed | Sensitively and Selectively Detect Biothiols by Using Fluorescence Method and Resonance Light Scattering Technique Simultaneously |
title_short | Sensitively and Selectively Detect Biothiols by Using Fluorescence Method and Resonance Light Scattering Technique Simultaneously |
title_sort | sensitively and selectively detect biothiols by using fluorescence method and resonance light scattering technique simultaneously |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891520/ https://www.ncbi.nlm.nih.gov/pubmed/31731646 http://dx.doi.org/10.3390/molecules24224136 |
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