Cargando…
A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy
Cordyceps Sinensis, a traditional Chinese medicine and a healthy food, has been used for the treatment of kidney disease for a long time. The aim of present study was to isolate a nucleoside/nucleobase-rich extract from Cordyceps Sinensis (CS-N), determine the contents of nucleosides and nucleobases...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891521/ https://www.ncbi.nlm.nih.gov/pubmed/31739543 http://dx.doi.org/10.3390/molecules24224119 |
_version_ | 1783475833573212160 |
---|---|
author | Dong, Zhonghua Sun, Yueyue Wei, Guangwei Li, Siying Zhao, Zhongxi |
author_facet | Dong, Zhonghua Sun, Yueyue Wei, Guangwei Li, Siying Zhao, Zhongxi |
author_sort | Dong, Zhonghua |
collection | PubMed |
description | Cordyceps Sinensis, a traditional Chinese medicine and a healthy food, has been used for the treatment of kidney disease for a long time. The aim of present study was to isolate a nucleoside/nucleobase-rich extract from Cordyceps Sinensis (CS-N), determine the contents of nucleosides and nucleobases, and explore its anti-diabetic nephropathy activity. CS-N was isolated and purified by using microporous resin and glucan columns and the unknown compounds were identified by using HPLC-DAD and LC-MS. The effects of CS-N on the epithelial–mesenchymal transition (EMT), extracellular matrix (ECM) depositions, and the MAPK signaling pathway were evaluated in streptozotocin (STZ)-induced diabetic mice and high glucose (HG)-exposed HK-2 cells. CS-N significantly attenuated the abnormity of renal functional parameters, ameliorated histopathological changes, and inhibited EMT and ECM accumulation by regulating p38/ERK signaling pathways. Our findings indicate that CS-N exerts a therapeutic effect on experimental diabetic renal fibrosis by mitigating the EMT and the subsequent ECM deposition with inhibition of p38 and ERK signaling pathways. |
format | Online Article Text |
id | pubmed-6891521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68915212019-12-18 A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy Dong, Zhonghua Sun, Yueyue Wei, Guangwei Li, Siying Zhao, Zhongxi Molecules Article Cordyceps Sinensis, a traditional Chinese medicine and a healthy food, has been used for the treatment of kidney disease for a long time. The aim of present study was to isolate a nucleoside/nucleobase-rich extract from Cordyceps Sinensis (CS-N), determine the contents of nucleosides and nucleobases, and explore its anti-diabetic nephropathy activity. CS-N was isolated and purified by using microporous resin and glucan columns and the unknown compounds were identified by using HPLC-DAD and LC-MS. The effects of CS-N on the epithelial–mesenchymal transition (EMT), extracellular matrix (ECM) depositions, and the MAPK signaling pathway were evaluated in streptozotocin (STZ)-induced diabetic mice and high glucose (HG)-exposed HK-2 cells. CS-N significantly attenuated the abnormity of renal functional parameters, ameliorated histopathological changes, and inhibited EMT and ECM accumulation by regulating p38/ERK signaling pathways. Our findings indicate that CS-N exerts a therapeutic effect on experimental diabetic renal fibrosis by mitigating the EMT and the subsequent ECM deposition with inhibition of p38 and ERK signaling pathways. MDPI 2019-11-14 /pmc/articles/PMC6891521/ /pubmed/31739543 http://dx.doi.org/10.3390/molecules24224119 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dong, Zhonghua Sun, Yueyue Wei, Guangwei Li, Siying Zhao, Zhongxi A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy |
title | A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy |
title_full | A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy |
title_fullStr | A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy |
title_full_unstemmed | A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy |
title_short | A Nucleoside/Nucleobase-Rich Extract from Cordyceps Sinensis Inhibits the Epithelial–Mesenchymal Transition and Protects against Renal Fibrosis in Diabetic Nephropathy |
title_sort | nucleoside/nucleobase-rich extract from cordyceps sinensis inhibits the epithelial–mesenchymal transition and protects against renal fibrosis in diabetic nephropathy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891521/ https://www.ncbi.nlm.nih.gov/pubmed/31739543 http://dx.doi.org/10.3390/molecules24224119 |
work_keys_str_mv | AT dongzhonghua anucleosidenucleobaserichextractfromcordycepssinensisinhibitstheepithelialmesenchymaltransitionandprotectsagainstrenalfibrosisindiabeticnephropathy AT sunyueyue anucleosidenucleobaserichextractfromcordycepssinensisinhibitstheepithelialmesenchymaltransitionandprotectsagainstrenalfibrosisindiabeticnephropathy AT weiguangwei anucleosidenucleobaserichextractfromcordycepssinensisinhibitstheepithelialmesenchymaltransitionandprotectsagainstrenalfibrosisindiabeticnephropathy AT lisiying anucleosidenucleobaserichextractfromcordycepssinensisinhibitstheepithelialmesenchymaltransitionandprotectsagainstrenalfibrosisindiabeticnephropathy AT zhaozhongxi anucleosidenucleobaserichextractfromcordycepssinensisinhibitstheepithelialmesenchymaltransitionandprotectsagainstrenalfibrosisindiabeticnephropathy AT dongzhonghua nucleosidenucleobaserichextractfromcordycepssinensisinhibitstheepithelialmesenchymaltransitionandprotectsagainstrenalfibrosisindiabeticnephropathy AT sunyueyue nucleosidenucleobaserichextractfromcordycepssinensisinhibitstheepithelialmesenchymaltransitionandprotectsagainstrenalfibrosisindiabeticnephropathy AT weiguangwei nucleosidenucleobaserichextractfromcordycepssinensisinhibitstheepithelialmesenchymaltransitionandprotectsagainstrenalfibrosisindiabeticnephropathy AT lisiying nucleosidenucleobaserichextractfromcordycepssinensisinhibitstheepithelialmesenchymaltransitionandprotectsagainstrenalfibrosisindiabeticnephropathy AT zhaozhongxi nucleosidenucleobaserichextractfromcordycepssinensisinhibitstheepithelialmesenchymaltransitionandprotectsagainstrenalfibrosisindiabeticnephropathy |