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Constructing Controllable Logic Circuits Based on DNAzyme Activity
Recently, DNA molecules have been widely used to construct advanced logic devices due to their unique properties, such as a simple structure and predictable behavior. In fact, there are still many challenges in the process of building logic circuits. Among them, the scalability of the logic circuit...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891523/ https://www.ncbi.nlm.nih.gov/pubmed/31731630 http://dx.doi.org/10.3390/molecules24224134 |
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author | Yang, Fengjie Liu, Yuan Wang, Bin Zhou, Changjun Zhang, Qiang |
author_facet | Yang, Fengjie Liu, Yuan Wang, Bin Zhou, Changjun Zhang, Qiang |
author_sort | Yang, Fengjie |
collection | PubMed |
description | Recently, DNA molecules have been widely used to construct advanced logic devices due to their unique properties, such as a simple structure and predictable behavior. In fact, there are still many challenges in the process of building logic circuits. Among them, the scalability of the logic circuit and the elimination of the crosstalk of the cascade circuit have become the focus of research. Inspired by biological allosteric regulation, we developed a controllable molecular logic circuit strategy based on the activity of DNAzyme. The E6 DNAzyme sequence was temporarily blocked by hairpin DNA and activated under appropriate input trigger conditions. Using a substrate with ribonucleobase (rA) modification as the detection strand, a series of binary basic logic gates (YES, AND, and INHIBIT) were implemented on the computational component platform. At the same time, we demonstrate a parallel demultiplexer and two multi-level cascade circuits (YES-YES and YES-Three input AND (YES-TAND)). In addition, the leakage of the cascade process was reduced by exploring factors such as concentration and DNA structure. The proposed DNAzyme activity regulation strategy provides great potential for the expansion of logic circuits in the future. |
format | Online Article Text |
id | pubmed-6891523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68915232019-12-18 Constructing Controllable Logic Circuits Based on DNAzyme Activity Yang, Fengjie Liu, Yuan Wang, Bin Zhou, Changjun Zhang, Qiang Molecules Article Recently, DNA molecules have been widely used to construct advanced logic devices due to their unique properties, such as a simple structure and predictable behavior. In fact, there are still many challenges in the process of building logic circuits. Among them, the scalability of the logic circuit and the elimination of the crosstalk of the cascade circuit have become the focus of research. Inspired by biological allosteric regulation, we developed a controllable molecular logic circuit strategy based on the activity of DNAzyme. The E6 DNAzyme sequence was temporarily blocked by hairpin DNA and activated under appropriate input trigger conditions. Using a substrate with ribonucleobase (rA) modification as the detection strand, a series of binary basic logic gates (YES, AND, and INHIBIT) were implemented on the computational component platform. At the same time, we demonstrate a parallel demultiplexer and two multi-level cascade circuits (YES-YES and YES-Three input AND (YES-TAND)). In addition, the leakage of the cascade process was reduced by exploring factors such as concentration and DNA structure. The proposed DNAzyme activity regulation strategy provides great potential for the expansion of logic circuits in the future. MDPI 2019-11-15 /pmc/articles/PMC6891523/ /pubmed/31731630 http://dx.doi.org/10.3390/molecules24224134 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Fengjie Liu, Yuan Wang, Bin Zhou, Changjun Zhang, Qiang Constructing Controllable Logic Circuits Based on DNAzyme Activity |
title | Constructing Controllable Logic Circuits Based on DNAzyme Activity |
title_full | Constructing Controllable Logic Circuits Based on DNAzyme Activity |
title_fullStr | Constructing Controllable Logic Circuits Based on DNAzyme Activity |
title_full_unstemmed | Constructing Controllable Logic Circuits Based on DNAzyme Activity |
title_short | Constructing Controllable Logic Circuits Based on DNAzyme Activity |
title_sort | constructing controllable logic circuits based on dnazyme activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891523/ https://www.ncbi.nlm.nih.gov/pubmed/31731630 http://dx.doi.org/10.3390/molecules24224134 |
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