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Porcine Hepatic Response to Fumonisin B(1) in a Short Exposure Period: Fatty Acid Profile and Clinical Investigations
Scarce studies have investigated the impact of fumonisin B(1) (FB(1)) on the hepatic tissue fatty acid (FA) profile, and no study is available on piglets. A 10-day in vivo experiment was performed on seven piglets/group: control and FB(1)-fed animals (diet was contaminated with fungal culture: 20 mg...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891595/ https://www.ncbi.nlm.nih.gov/pubmed/31717687 http://dx.doi.org/10.3390/toxins11110655 |
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author | Ali, Omeralfaroug Szabó-Fodor, Judit Fébel, Hedvig Mézes, Miklós Balogh, Krisztián Glávits, Róbert Kovács, Melinda Zantomasi, Arianna Szabó, András |
author_facet | Ali, Omeralfaroug Szabó-Fodor, Judit Fébel, Hedvig Mézes, Miklós Balogh, Krisztián Glávits, Róbert Kovács, Melinda Zantomasi, Arianna Szabó, András |
author_sort | Ali, Omeralfaroug |
collection | PubMed |
description | Scarce studies have investigated the impact of fumonisin B(1) (FB(1)) on the hepatic tissue fatty acid (FA) profile, and no study is available on piglets. A 10-day in vivo experiment was performed on seven piglets/group: control and FB(1)-fed animals (diet was contaminated with fungal culture: 20 mg FB(1)/kg diet). Independent sample t-test was carried out at p < 0.05 as the significance level. Neither growth, nor feed efficiency, was affected. The hepatic phospholipid (PL) fatty acids (FAs) were more susceptible for FB(1), while triglyceride (TG) was less responsive. The impact of FB(1) on hepatic PL polyunsaturated fatty acids (PUFAs) was more pronounced than on saturated fatty acids. Among all PUFAs, predominant ones in response were docosapentaenoicacid (DPA) (↓), docosahexaenoic DHA (↓) and arachidonic acids (↑). This led to a higher omega-6:omega-3 ratio, whereas a similar finding was noted in TGs. Neither total saturation (SFA) nor total monousaturation (MUFA) were affected by the FB(1) administration. The liver showed an increase in malondialdehyde, as well as antioxidant capacity (reduced glutathione and glutathione peroxidase). The plasma enzymatic assessment revealed an increase in alkaline phosphatase (ALP), while alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH), and gamma-glutamyltransferase (GGT) were not influenced. The microscopic sections provided evidence of vacuolar degeneration of the hepatocytes’ cytoplasm, but it was not severe. Furthermore, the lung edema was developed, while the kidney was not affected. In conclusion, regarding FB(1)-mediated hepatotoxicity in piglets, the potential effect of slight hepatotoxicity did not compromise growth performance, at least at the dose and exposure period applied. |
format | Online Article Text |
id | pubmed-6891595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68915952019-12-12 Porcine Hepatic Response to Fumonisin B(1) in a Short Exposure Period: Fatty Acid Profile and Clinical Investigations Ali, Omeralfaroug Szabó-Fodor, Judit Fébel, Hedvig Mézes, Miklós Balogh, Krisztián Glávits, Róbert Kovács, Melinda Zantomasi, Arianna Szabó, András Toxins (Basel) Article Scarce studies have investigated the impact of fumonisin B(1) (FB(1)) on the hepatic tissue fatty acid (FA) profile, and no study is available on piglets. A 10-day in vivo experiment was performed on seven piglets/group: control and FB(1)-fed animals (diet was contaminated with fungal culture: 20 mg FB(1)/kg diet). Independent sample t-test was carried out at p < 0.05 as the significance level. Neither growth, nor feed efficiency, was affected. The hepatic phospholipid (PL) fatty acids (FAs) were more susceptible for FB(1), while triglyceride (TG) was less responsive. The impact of FB(1) on hepatic PL polyunsaturated fatty acids (PUFAs) was more pronounced than on saturated fatty acids. Among all PUFAs, predominant ones in response were docosapentaenoicacid (DPA) (↓), docosahexaenoic DHA (↓) and arachidonic acids (↑). This led to a higher omega-6:omega-3 ratio, whereas a similar finding was noted in TGs. Neither total saturation (SFA) nor total monousaturation (MUFA) were affected by the FB(1) administration. The liver showed an increase in malondialdehyde, as well as antioxidant capacity (reduced glutathione and glutathione peroxidase). The plasma enzymatic assessment revealed an increase in alkaline phosphatase (ALP), while alanine transaminase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH), and gamma-glutamyltransferase (GGT) were not influenced. The microscopic sections provided evidence of vacuolar degeneration of the hepatocytes’ cytoplasm, but it was not severe. Furthermore, the lung edema was developed, while the kidney was not affected. In conclusion, regarding FB(1)-mediated hepatotoxicity in piglets, the potential effect of slight hepatotoxicity did not compromise growth performance, at least at the dose and exposure period applied. MDPI 2019-11-10 /pmc/articles/PMC6891595/ /pubmed/31717687 http://dx.doi.org/10.3390/toxins11110655 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ali, Omeralfaroug Szabó-Fodor, Judit Fébel, Hedvig Mézes, Miklós Balogh, Krisztián Glávits, Róbert Kovács, Melinda Zantomasi, Arianna Szabó, András Porcine Hepatic Response to Fumonisin B(1) in a Short Exposure Period: Fatty Acid Profile and Clinical Investigations |
title | Porcine Hepatic Response to Fumonisin B(1) in a Short Exposure Period: Fatty Acid Profile and Clinical Investigations |
title_full | Porcine Hepatic Response to Fumonisin B(1) in a Short Exposure Period: Fatty Acid Profile and Clinical Investigations |
title_fullStr | Porcine Hepatic Response to Fumonisin B(1) in a Short Exposure Period: Fatty Acid Profile and Clinical Investigations |
title_full_unstemmed | Porcine Hepatic Response to Fumonisin B(1) in a Short Exposure Period: Fatty Acid Profile and Clinical Investigations |
title_short | Porcine Hepatic Response to Fumonisin B(1) in a Short Exposure Period: Fatty Acid Profile and Clinical Investigations |
title_sort | porcine hepatic response to fumonisin b(1) in a short exposure period: fatty acid profile and clinical investigations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891595/ https://www.ncbi.nlm.nih.gov/pubmed/31717687 http://dx.doi.org/10.3390/toxins11110655 |
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