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Echinochrome A Reduces Colitis in Mice and Induces In Vitro Generation of Regulatory Immune Cells

Echinochrome A (Ech A), a natural pigment extracted from sea urchins, is the active ingredient of a marine-derived pharmaceutical called ‘histochrome’. Since it exhibits several biological activities including anti-oxidative and anti-inflammatory effects, it has been applied to the management of car...

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Autores principales: Oh, Su-Jeong, Seo, Yoojin, Ahn, Ji-Su, Shin, Ye Young, Yang, Ji Won, Kim, Hyoung Kyu, Han, Jin, Mishchenko, Natalia P., Fedoreyev, Sergey A., Stonik, Valentin A., Kim, Hyung-Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891633/
https://www.ncbi.nlm.nih.gov/pubmed/31683521
http://dx.doi.org/10.3390/md17110622
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author Oh, Su-Jeong
Seo, Yoojin
Ahn, Ji-Su
Shin, Ye Young
Yang, Ji Won
Kim, Hyoung Kyu
Han, Jin
Mishchenko, Natalia P.
Fedoreyev, Sergey A.
Stonik, Valentin A.
Kim, Hyung-Sik
author_facet Oh, Su-Jeong
Seo, Yoojin
Ahn, Ji-Su
Shin, Ye Young
Yang, Ji Won
Kim, Hyoung Kyu
Han, Jin
Mishchenko, Natalia P.
Fedoreyev, Sergey A.
Stonik, Valentin A.
Kim, Hyung-Sik
author_sort Oh, Su-Jeong
collection PubMed
description Echinochrome A (Ech A), a natural pigment extracted from sea urchins, is the active ingredient of a marine-derived pharmaceutical called ‘histochrome’. Since it exhibits several biological activities including anti-oxidative and anti-inflammatory effects, it has been applied to the management of cardiac injury and ocular degenerative disorders in Russia and its protective role has been studied for other pathologic conditions. In the present study, we sought to investigate the therapeutic potential of Ech A for inflammatory bowel disease (IBD) using a murine model of experimental colitis. We found that intravenous injection of Ech A significantly prevented body weight loss and subsequent lethality in colitis-induced mice. Interestingly, T cell proliferation was significantly inhibited upon Ech A treatment in vitro. During the helper T (Th) cell differentiation process, Ech A stimulated the generation regulatory T (Treg) cells that modulate the inflammatory response and immune homeostasis. Moreover, Ech A treatment suppressed the in vitro activation of pro-inflammatory M1 type macrophages, while inducing the production of M2 type macrophages that promote the resolution of inflammation and initiate tissue repair. Based on these results, we suggest that Ech A could provide a beneficial impact on IBD by correcting the imbalance in the intestinal immune system.
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spelling pubmed-68916332019-12-12 Echinochrome A Reduces Colitis in Mice and Induces In Vitro Generation of Regulatory Immune Cells Oh, Su-Jeong Seo, Yoojin Ahn, Ji-Su Shin, Ye Young Yang, Ji Won Kim, Hyoung Kyu Han, Jin Mishchenko, Natalia P. Fedoreyev, Sergey A. Stonik, Valentin A. Kim, Hyung-Sik Mar Drugs Article Echinochrome A (Ech A), a natural pigment extracted from sea urchins, is the active ingredient of a marine-derived pharmaceutical called ‘histochrome’. Since it exhibits several biological activities including anti-oxidative and anti-inflammatory effects, it has been applied to the management of cardiac injury and ocular degenerative disorders in Russia and its protective role has been studied for other pathologic conditions. In the present study, we sought to investigate the therapeutic potential of Ech A for inflammatory bowel disease (IBD) using a murine model of experimental colitis. We found that intravenous injection of Ech A significantly prevented body weight loss and subsequent lethality in colitis-induced mice. Interestingly, T cell proliferation was significantly inhibited upon Ech A treatment in vitro. During the helper T (Th) cell differentiation process, Ech A stimulated the generation regulatory T (Treg) cells that modulate the inflammatory response and immune homeostasis. Moreover, Ech A treatment suppressed the in vitro activation of pro-inflammatory M1 type macrophages, while inducing the production of M2 type macrophages that promote the resolution of inflammation and initiate tissue repair. Based on these results, we suggest that Ech A could provide a beneficial impact on IBD by correcting the imbalance in the intestinal immune system. MDPI 2019-10-31 /pmc/articles/PMC6891633/ /pubmed/31683521 http://dx.doi.org/10.3390/md17110622 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oh, Su-Jeong
Seo, Yoojin
Ahn, Ji-Su
Shin, Ye Young
Yang, Ji Won
Kim, Hyoung Kyu
Han, Jin
Mishchenko, Natalia P.
Fedoreyev, Sergey A.
Stonik, Valentin A.
Kim, Hyung-Sik
Echinochrome A Reduces Colitis in Mice and Induces In Vitro Generation of Regulatory Immune Cells
title Echinochrome A Reduces Colitis in Mice and Induces In Vitro Generation of Regulatory Immune Cells
title_full Echinochrome A Reduces Colitis in Mice and Induces In Vitro Generation of Regulatory Immune Cells
title_fullStr Echinochrome A Reduces Colitis in Mice and Induces In Vitro Generation of Regulatory Immune Cells
title_full_unstemmed Echinochrome A Reduces Colitis in Mice and Induces In Vitro Generation of Regulatory Immune Cells
title_short Echinochrome A Reduces Colitis in Mice and Induces In Vitro Generation of Regulatory Immune Cells
title_sort echinochrome a reduces colitis in mice and induces in vitro generation of regulatory immune cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891633/
https://www.ncbi.nlm.nih.gov/pubmed/31683521
http://dx.doi.org/10.3390/md17110622
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