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Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices

In order for automatic microinjection to serve biomedical and genetic research, we have designed and manufactured a PDMS-based sensor with a circular section channel using the microwire molding technique. For the very precise control of microfluidic transport, we developed a microfluidic pulse width...

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Autores principales: Ardeleanu, Mihăiţă Nicolae, Popescu, Ileana Nicoleta, Udroiu, Iulian Nicolae, Diaconu, Emil Mihai, Mihai, Simona, Lungu, Emil, Alhalaili, Badriyah, Vidu, Ruxandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891790/
https://www.ncbi.nlm.nih.gov/pubmed/31717452
http://dx.doi.org/10.3390/s19224886
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author Ardeleanu, Mihăiţă Nicolae
Popescu, Ileana Nicoleta
Udroiu, Iulian Nicolae
Diaconu, Emil Mihai
Mihai, Simona
Lungu, Emil
Alhalaili, Badriyah
Vidu, Ruxandra
author_facet Ardeleanu, Mihăiţă Nicolae
Popescu, Ileana Nicoleta
Udroiu, Iulian Nicolae
Diaconu, Emil Mihai
Mihai, Simona
Lungu, Emil
Alhalaili, Badriyah
Vidu, Ruxandra
author_sort Ardeleanu, Mihăiţă Nicolae
collection PubMed
description In order for automatic microinjection to serve biomedical and genetic research, we have designed and manufactured a PDMS-based sensor with a circular section channel using the microwire molding technique. For the very precise control of microfluidic transport, we developed a microfluidic pulse width modulation system (MPWM) for automatic microinjections at a picoliter level. By adding a computer-aided detection and tracking of fluid-specific elements in the microfluidic circuit, the PDMS microchannel sensor became the basic element in the automatic control of the microinjection sensor. With the PDMS microinjection sensor, we precise measured microfluidic volumes under visual detection, assisted by very precise computer equipment (with precision below 1 μm) based on image processing. The calibration of the MPWM system was performed to increase the reproducibility of the results and to detect and measure microfluidic volumes. The novel PDMS-based sensor system for MPWM measurements of microfluidic volumes contributes to the advancement of intelligent control methods and techniques, which could lead to new developments in the design, control, and in applications of real-time intelligent sensor system control.
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spelling pubmed-68917902019-12-12 Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices Ardeleanu, Mihăiţă Nicolae Popescu, Ileana Nicoleta Udroiu, Iulian Nicolae Diaconu, Emil Mihai Mihai, Simona Lungu, Emil Alhalaili, Badriyah Vidu, Ruxandra Sensors (Basel) Article In order for automatic microinjection to serve biomedical and genetic research, we have designed and manufactured a PDMS-based sensor with a circular section channel using the microwire molding technique. For the very precise control of microfluidic transport, we developed a microfluidic pulse width modulation system (MPWM) for automatic microinjections at a picoliter level. By adding a computer-aided detection and tracking of fluid-specific elements in the microfluidic circuit, the PDMS microchannel sensor became the basic element in the automatic control of the microinjection sensor. With the PDMS microinjection sensor, we precise measured microfluidic volumes under visual detection, assisted by very precise computer equipment (with precision below 1 μm) based on image processing. The calibration of the MPWM system was performed to increase the reproducibility of the results and to detect and measure microfluidic volumes. The novel PDMS-based sensor system for MPWM measurements of microfluidic volumes contributes to the advancement of intelligent control methods and techniques, which could lead to new developments in the design, control, and in applications of real-time intelligent sensor system control. MDPI 2019-11-08 /pmc/articles/PMC6891790/ /pubmed/31717452 http://dx.doi.org/10.3390/s19224886 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ardeleanu, Mihăiţă Nicolae
Popescu, Ileana Nicoleta
Udroiu, Iulian Nicolae
Diaconu, Emil Mihai
Mihai, Simona
Lungu, Emil
Alhalaili, Badriyah
Vidu, Ruxandra
Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices
title Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices
title_full Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices
title_fullStr Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices
title_full_unstemmed Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices
title_short Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices
title_sort novel pdms-based sensor system for mpwm measurements of picoliter volumes in microfluidic devices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891790/
https://www.ncbi.nlm.nih.gov/pubmed/31717452
http://dx.doi.org/10.3390/s19224886
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