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Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices
In order for automatic microinjection to serve biomedical and genetic research, we have designed and manufactured a PDMS-based sensor with a circular section channel using the microwire molding technique. For the very precise control of microfluidic transport, we developed a microfluidic pulse width...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891790/ https://www.ncbi.nlm.nih.gov/pubmed/31717452 http://dx.doi.org/10.3390/s19224886 |
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author | Ardeleanu, Mihăiţă Nicolae Popescu, Ileana Nicoleta Udroiu, Iulian Nicolae Diaconu, Emil Mihai Mihai, Simona Lungu, Emil Alhalaili, Badriyah Vidu, Ruxandra |
author_facet | Ardeleanu, Mihăiţă Nicolae Popescu, Ileana Nicoleta Udroiu, Iulian Nicolae Diaconu, Emil Mihai Mihai, Simona Lungu, Emil Alhalaili, Badriyah Vidu, Ruxandra |
author_sort | Ardeleanu, Mihăiţă Nicolae |
collection | PubMed |
description | In order for automatic microinjection to serve biomedical and genetic research, we have designed and manufactured a PDMS-based sensor with a circular section channel using the microwire molding technique. For the very precise control of microfluidic transport, we developed a microfluidic pulse width modulation system (MPWM) for automatic microinjections at a picoliter level. By adding a computer-aided detection and tracking of fluid-specific elements in the microfluidic circuit, the PDMS microchannel sensor became the basic element in the automatic control of the microinjection sensor. With the PDMS microinjection sensor, we precise measured microfluidic volumes under visual detection, assisted by very precise computer equipment (with precision below 1 μm) based on image processing. The calibration of the MPWM system was performed to increase the reproducibility of the results and to detect and measure microfluidic volumes. The novel PDMS-based sensor system for MPWM measurements of microfluidic volumes contributes to the advancement of intelligent control methods and techniques, which could lead to new developments in the design, control, and in applications of real-time intelligent sensor system control. |
format | Online Article Text |
id | pubmed-6891790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68917902019-12-12 Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices Ardeleanu, Mihăiţă Nicolae Popescu, Ileana Nicoleta Udroiu, Iulian Nicolae Diaconu, Emil Mihai Mihai, Simona Lungu, Emil Alhalaili, Badriyah Vidu, Ruxandra Sensors (Basel) Article In order for automatic microinjection to serve biomedical and genetic research, we have designed and manufactured a PDMS-based sensor with a circular section channel using the microwire molding technique. For the very precise control of microfluidic transport, we developed a microfluidic pulse width modulation system (MPWM) for automatic microinjections at a picoliter level. By adding a computer-aided detection and tracking of fluid-specific elements in the microfluidic circuit, the PDMS microchannel sensor became the basic element in the automatic control of the microinjection sensor. With the PDMS microinjection sensor, we precise measured microfluidic volumes under visual detection, assisted by very precise computer equipment (with precision below 1 μm) based on image processing. The calibration of the MPWM system was performed to increase the reproducibility of the results and to detect and measure microfluidic volumes. The novel PDMS-based sensor system for MPWM measurements of microfluidic volumes contributes to the advancement of intelligent control methods and techniques, which could lead to new developments in the design, control, and in applications of real-time intelligent sensor system control. MDPI 2019-11-08 /pmc/articles/PMC6891790/ /pubmed/31717452 http://dx.doi.org/10.3390/s19224886 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ardeleanu, Mihăiţă Nicolae Popescu, Ileana Nicoleta Udroiu, Iulian Nicolae Diaconu, Emil Mihai Mihai, Simona Lungu, Emil Alhalaili, Badriyah Vidu, Ruxandra Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices |
title | Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices |
title_full | Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices |
title_fullStr | Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices |
title_full_unstemmed | Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices |
title_short | Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices |
title_sort | novel pdms-based sensor system for mpwm measurements of picoliter volumes in microfluidic devices |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891790/ https://www.ncbi.nlm.nih.gov/pubmed/31717452 http://dx.doi.org/10.3390/s19224886 |
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