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CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses

T cells secrete bioactive extracellular vesicles (EVs), but the potential biological effects of CD4(+) T cell EVs are not clear. The main purpose of this study is to investigate the effects of CD4(+) T cell–derived EVs on B cell responses and examine their role in antigen‐mediated humoral immune res...

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Autores principales: Lu, Jian, Wu, Jing, Xie, Feiting, Tian, Jie, Tang, Xinyi, Guo, Hongye, Ma, Jie, Xu, Ping, Mao, Lingxiang, Xu, Huaxi, Wang, Shengjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891927/
https://www.ncbi.nlm.nih.gov/pubmed/31832305
http://dx.doi.org/10.1002/advs.201802219
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author Lu, Jian
Wu, Jing
Xie, Feiting
Tian, Jie
Tang, Xinyi
Guo, Hongye
Ma, Jie
Xu, Ping
Mao, Lingxiang
Xu, Huaxi
Wang, Shengjun
author_facet Lu, Jian
Wu, Jing
Xie, Feiting
Tian, Jie
Tang, Xinyi
Guo, Hongye
Ma, Jie
Xu, Ping
Mao, Lingxiang
Xu, Huaxi
Wang, Shengjun
author_sort Lu, Jian
collection PubMed
description T cells secrete bioactive extracellular vesicles (EVs), but the potential biological effects of CD4(+) T cell EVs are not clear. The main purpose of this study is to investigate the effects of CD4(+) T cell–derived EVs on B cell responses and examine their role in antigen‐mediated humoral immune responses. In this study, CD4(+) T cell EVs are purified from activated CD4(+) T cells in vitro. After immunization with the Hepatitis B surface antigen (HBsAg) vaccine, CD4(+) T cell EVs‐treated mice show stronger humoral immune responses, which is indicated by a greater Hepatitis B surface antibody (HBsAb) level in serum and a greater proportion of plasma cells in bone marrow. In addition, it is found that EVs released from activated CD4(+) T cells play an important role in B cell responses in vitro, which significantly promote B cell activation, proliferation, and antibody production. Interestingly, antigen‐specific CD4(+) T cell EVs are found to be more efficient than control EVs in enhancing B cell responses. Furthermore, it is shown that CD40 ligand (CD40L) is involved in CD4(+) T cell EVs‐mediated B cell responses. Overall, the results have demonstrated that CD4(+) T cell EVs enhance B cell responses and serve as a novel immunomodulator to promote antigen‐specific humoral immune responses.
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spelling pubmed-68919272019-12-12 CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses Lu, Jian Wu, Jing Xie, Feiting Tian, Jie Tang, Xinyi Guo, Hongye Ma, Jie Xu, Ping Mao, Lingxiang Xu, Huaxi Wang, Shengjun Adv Sci (Weinh) Full Papers T cells secrete bioactive extracellular vesicles (EVs), but the potential biological effects of CD4(+) T cell EVs are not clear. The main purpose of this study is to investigate the effects of CD4(+) T cell–derived EVs on B cell responses and examine their role in antigen‐mediated humoral immune responses. In this study, CD4(+) T cell EVs are purified from activated CD4(+) T cells in vitro. After immunization with the Hepatitis B surface antigen (HBsAg) vaccine, CD4(+) T cell EVs‐treated mice show stronger humoral immune responses, which is indicated by a greater Hepatitis B surface antibody (HBsAb) level in serum and a greater proportion of plasma cells in bone marrow. In addition, it is found that EVs released from activated CD4(+) T cells play an important role in B cell responses in vitro, which significantly promote B cell activation, proliferation, and antibody production. Interestingly, antigen‐specific CD4(+) T cell EVs are found to be more efficient than control EVs in enhancing B cell responses. Furthermore, it is shown that CD40 ligand (CD40L) is involved in CD4(+) T cell EVs‐mediated B cell responses. Overall, the results have demonstrated that CD4(+) T cell EVs enhance B cell responses and serve as a novel immunomodulator to promote antigen‐specific humoral immune responses. John Wiley and Sons Inc. 2019-09-30 /pmc/articles/PMC6891927/ /pubmed/31832305 http://dx.doi.org/10.1002/advs.201802219 Text en © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Lu, Jian
Wu, Jing
Xie, Feiting
Tian, Jie
Tang, Xinyi
Guo, Hongye
Ma, Jie
Xu, Ping
Mao, Lingxiang
Xu, Huaxi
Wang, Shengjun
CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses
title CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses
title_full CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses
title_fullStr CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses
title_full_unstemmed CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses
title_short CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses
title_sort cd4(+) t cell‐released extracellular vesicles potentiate the efficacy of the hbsag vaccine by enhancing b cell responses
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891927/
https://www.ncbi.nlm.nih.gov/pubmed/31832305
http://dx.doi.org/10.1002/advs.201802219
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