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CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses
T cells secrete bioactive extracellular vesicles (EVs), but the potential biological effects of CD4(+) T cell EVs are not clear. The main purpose of this study is to investigate the effects of CD4(+) T cell–derived EVs on B cell responses and examine their role in antigen‐mediated humoral immune res...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891927/ https://www.ncbi.nlm.nih.gov/pubmed/31832305 http://dx.doi.org/10.1002/advs.201802219 |
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author | Lu, Jian Wu, Jing Xie, Feiting Tian, Jie Tang, Xinyi Guo, Hongye Ma, Jie Xu, Ping Mao, Lingxiang Xu, Huaxi Wang, Shengjun |
author_facet | Lu, Jian Wu, Jing Xie, Feiting Tian, Jie Tang, Xinyi Guo, Hongye Ma, Jie Xu, Ping Mao, Lingxiang Xu, Huaxi Wang, Shengjun |
author_sort | Lu, Jian |
collection | PubMed |
description | T cells secrete bioactive extracellular vesicles (EVs), but the potential biological effects of CD4(+) T cell EVs are not clear. The main purpose of this study is to investigate the effects of CD4(+) T cell–derived EVs on B cell responses and examine their role in antigen‐mediated humoral immune responses. In this study, CD4(+) T cell EVs are purified from activated CD4(+) T cells in vitro. After immunization with the Hepatitis B surface antigen (HBsAg) vaccine, CD4(+) T cell EVs‐treated mice show stronger humoral immune responses, which is indicated by a greater Hepatitis B surface antibody (HBsAb) level in serum and a greater proportion of plasma cells in bone marrow. In addition, it is found that EVs released from activated CD4(+) T cells play an important role in B cell responses in vitro, which significantly promote B cell activation, proliferation, and antibody production. Interestingly, antigen‐specific CD4(+) T cell EVs are found to be more efficient than control EVs in enhancing B cell responses. Furthermore, it is shown that CD40 ligand (CD40L) is involved in CD4(+) T cell EVs‐mediated B cell responses. Overall, the results have demonstrated that CD4(+) T cell EVs enhance B cell responses and serve as a novel immunomodulator to promote antigen‐specific humoral immune responses. |
format | Online Article Text |
id | pubmed-6891927 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68919272019-12-12 CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses Lu, Jian Wu, Jing Xie, Feiting Tian, Jie Tang, Xinyi Guo, Hongye Ma, Jie Xu, Ping Mao, Lingxiang Xu, Huaxi Wang, Shengjun Adv Sci (Weinh) Full Papers T cells secrete bioactive extracellular vesicles (EVs), but the potential biological effects of CD4(+) T cell EVs are not clear. The main purpose of this study is to investigate the effects of CD4(+) T cell–derived EVs on B cell responses and examine their role in antigen‐mediated humoral immune responses. In this study, CD4(+) T cell EVs are purified from activated CD4(+) T cells in vitro. After immunization with the Hepatitis B surface antigen (HBsAg) vaccine, CD4(+) T cell EVs‐treated mice show stronger humoral immune responses, which is indicated by a greater Hepatitis B surface antibody (HBsAb) level in serum and a greater proportion of plasma cells in bone marrow. In addition, it is found that EVs released from activated CD4(+) T cells play an important role in B cell responses in vitro, which significantly promote B cell activation, proliferation, and antibody production. Interestingly, antigen‐specific CD4(+) T cell EVs are found to be more efficient than control EVs in enhancing B cell responses. Furthermore, it is shown that CD40 ligand (CD40L) is involved in CD4(+) T cell EVs‐mediated B cell responses. Overall, the results have demonstrated that CD4(+) T cell EVs enhance B cell responses and serve as a novel immunomodulator to promote antigen‐specific humoral immune responses. John Wiley and Sons Inc. 2019-09-30 /pmc/articles/PMC6891927/ /pubmed/31832305 http://dx.doi.org/10.1002/advs.201802219 Text en © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Lu, Jian Wu, Jing Xie, Feiting Tian, Jie Tang, Xinyi Guo, Hongye Ma, Jie Xu, Ping Mao, Lingxiang Xu, Huaxi Wang, Shengjun CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses |
title | CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses |
title_full | CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses |
title_fullStr | CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses |
title_full_unstemmed | CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses |
title_short | CD4(+) T Cell‐Released Extracellular Vesicles Potentiate the Efficacy of the HBsAg Vaccine by Enhancing B Cell Responses |
title_sort | cd4(+) t cell‐released extracellular vesicles potentiate the efficacy of the hbsag vaccine by enhancing b cell responses |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6891927/ https://www.ncbi.nlm.nih.gov/pubmed/31832305 http://dx.doi.org/10.1002/advs.201802219 |
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