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Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans

Animals change their behaviors in response to external stimuli, and numerous neurotransmitters are involved in these behavioral changes. In Caenorhabditis elegans, serotonin (5-HT) affects various behaviors such as inhibition of locomotion, stimulation of egg laying, and pharyngeal pumping. Previous...

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Autores principales: Mori, Haruka, Ashida, Keita, Shidara, Hisashi, Nikai, Tatsuya, Hotta, Kohji, Oka, Kotaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892467/
https://www.ncbi.nlm.nih.gov/pubmed/31800640
http://dx.doi.org/10.1371/journal.pone.0226044
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author Mori, Haruka
Ashida, Keita
Shidara, Hisashi
Nikai, Tatsuya
Hotta, Kohji
Oka, Kotaro
author_facet Mori, Haruka
Ashida, Keita
Shidara, Hisashi
Nikai, Tatsuya
Hotta, Kohji
Oka, Kotaro
author_sort Mori, Haruka
collection PubMed
description Animals change their behaviors in response to external stimuli, and numerous neurotransmitters are involved in these behavioral changes. In Caenorhabditis elegans, serotonin (5-HT) affects various behaviors such as inhibition of locomotion, stimulation of egg laying, and pharyngeal pumping. Previous research has shown that the neural activity of the RID interneuron increases when the worm moves forward, and the RID is necessary for sustaining forward locomotion. However, the relationship between 5-HT and neural activity of RID, and how it modulates the behavior of the worm has not been investigated. In this article, we reveal the relationship among 5-HT, RID activity, and the behavior of worms using a custom-made tracking and imaging system. We simultaneously measured the neural activity of the RID and behavior in worms with three conditions: mock animals, animals pre-exposed to 5-HT, and 5-HT receptor mod-1 mutants. As shown in previous research, the neural activity of the RID increased during the transition from backward to forward, whereas it decreased during the transition from forward to backward in mock animals. These changes in neural activity were not observed in animals pre-exposed to 5-HT and mod-1 mutants. Moreover, RID activity was correlated with the velocity of the worm in mock animals. However, this correlation was not observed in animals pre-exposed to 5-HT and mod-1 mutants. Our results demonstrate that 5-HT modulates the activity of the RID interneuron, and we infer that the RID plays a role in modulating forward locomotion by changing its activity through 5-HT.
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spelling pubmed-68924672019-12-14 Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans Mori, Haruka Ashida, Keita Shidara, Hisashi Nikai, Tatsuya Hotta, Kohji Oka, Kotaro PLoS One Research Article Animals change their behaviors in response to external stimuli, and numerous neurotransmitters are involved in these behavioral changes. In Caenorhabditis elegans, serotonin (5-HT) affects various behaviors such as inhibition of locomotion, stimulation of egg laying, and pharyngeal pumping. Previous research has shown that the neural activity of the RID interneuron increases when the worm moves forward, and the RID is necessary for sustaining forward locomotion. However, the relationship between 5-HT and neural activity of RID, and how it modulates the behavior of the worm has not been investigated. In this article, we reveal the relationship among 5-HT, RID activity, and the behavior of worms using a custom-made tracking and imaging system. We simultaneously measured the neural activity of the RID and behavior in worms with three conditions: mock animals, animals pre-exposed to 5-HT, and 5-HT receptor mod-1 mutants. As shown in previous research, the neural activity of the RID increased during the transition from backward to forward, whereas it decreased during the transition from forward to backward in mock animals. These changes in neural activity were not observed in animals pre-exposed to 5-HT and mod-1 mutants. Moreover, RID activity was correlated with the velocity of the worm in mock animals. However, this correlation was not observed in animals pre-exposed to 5-HT and mod-1 mutants. Our results demonstrate that 5-HT modulates the activity of the RID interneuron, and we infer that the RID plays a role in modulating forward locomotion by changing its activity through 5-HT. Public Library of Science 2019-12-04 /pmc/articles/PMC6892467/ /pubmed/31800640 http://dx.doi.org/10.1371/journal.pone.0226044 Text en © 2019 Mori et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mori, Haruka
Ashida, Keita
Shidara, Hisashi
Nikai, Tatsuya
Hotta, Kohji
Oka, Kotaro
Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans
title Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans
title_full Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans
title_fullStr Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans
title_full_unstemmed Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans
title_short Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans
title_sort serotonin modulates behavior-related neural activity of rid interneuron in caenorhabditis elegans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892467/
https://www.ncbi.nlm.nih.gov/pubmed/31800640
http://dx.doi.org/10.1371/journal.pone.0226044
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