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Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo

BACKGROUND: Ant venoms express surface molecules that participate in antigen presentation involving pro- and anti-inflammatory cytokines. This work aims to investigate the expression of MHC-II, CD80 and CD86 on the polymorphonuclear cells (PMNs) in rats injected with samsum ant venom (SAV). METHODS:...

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Autores principales: Ebaid, Hossam, Abdel-Salam, Bahaa, Alhazza, Ibrahim, Al-Tamimi, Jameel, Hassan, Iftekhar, Rady, Ahmed, Mashaly, Ashraf, Mahmoud, Ahmed, Sammour, Reda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centro de Estudos de Venenos e Animais Peçonhentos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892565/
https://www.ncbi.nlm.nih.gov/pubmed/31839800
http://dx.doi.org/10.1590/1678-9199-JVATITD-2019-0020
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author Ebaid, Hossam
Abdel-Salam, Bahaa
Alhazza, Ibrahim
Al-Tamimi, Jameel
Hassan, Iftekhar
Rady, Ahmed
Mashaly, Ashraf
Mahmoud, Ahmed
Sammour, Reda
author_facet Ebaid, Hossam
Abdel-Salam, Bahaa
Alhazza, Ibrahim
Al-Tamimi, Jameel
Hassan, Iftekhar
Rady, Ahmed
Mashaly, Ashraf
Mahmoud, Ahmed
Sammour, Reda
author_sort Ebaid, Hossam
collection PubMed
description BACKGROUND: Ant venoms express surface molecules that participate in antigen presentation involving pro- and anti-inflammatory cytokines. This work aims to investigate the expression of MHC-II, CD80 and CD86 on the polymorphonuclear cells (PMNs) in rats injected with samsum ant venom (SAV). METHODS: Rats were divided into three groups - control, SAV-treated (intraperitoneal route, 600 μg/kg), and SAV-treated (subcutaneous route, 600 μg/kg). After five doses, animals were euthanized and samples collected for analysis. RESULTS: The subcutaneous SAV-trated rats presented decreased levels of glutathione with increased cholesterol and triglyceride levels. Intraperitoneal SAV-treated animals displayed significantly reduced concentrations of both IFN-γ and IL-17 in comparison with the control group. However, intraperitoneal and subcutaneous SAV-treated rats were able to upregulate the expressions of MHC-II, CD80 and CD86 on PMNs in comparison with the control respectively. The histological examination showed severe lymphocyte depletion in the splenic white pulp of the intraperitoneal SAV-injected rats. CONCLUSION: Stimulation of PMNs by SAV leads to upregulation of MHC-II, CD 80, and CD 86, which plays critical roles in antigen presentation and consequently proliferation of T-cells. Subcutaneous route was more efficient than intraperitoneal by elevating MHC-II, CD80 and CD86 expression, disturbing oxidative stability and increasing lipogram concentration.
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spelling pubmed-68925652019-12-13 Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo Ebaid, Hossam Abdel-Salam, Bahaa Alhazza, Ibrahim Al-Tamimi, Jameel Hassan, Iftekhar Rady, Ahmed Mashaly, Ashraf Mahmoud, Ahmed Sammour, Reda J Venom Anim Toxins Incl Trop Dis Research BACKGROUND: Ant venoms express surface molecules that participate in antigen presentation involving pro- and anti-inflammatory cytokines. This work aims to investigate the expression of MHC-II, CD80 and CD86 on the polymorphonuclear cells (PMNs) in rats injected with samsum ant venom (SAV). METHODS: Rats were divided into three groups - control, SAV-treated (intraperitoneal route, 600 μg/kg), and SAV-treated (subcutaneous route, 600 μg/kg). After five doses, animals were euthanized and samples collected for analysis. RESULTS: The subcutaneous SAV-trated rats presented decreased levels of glutathione with increased cholesterol and triglyceride levels. Intraperitoneal SAV-treated animals displayed significantly reduced concentrations of both IFN-γ and IL-17 in comparison with the control group. However, intraperitoneal and subcutaneous SAV-treated rats were able to upregulate the expressions of MHC-II, CD80 and CD86 on PMNs in comparison with the control respectively. The histological examination showed severe lymphocyte depletion in the splenic white pulp of the intraperitoneal SAV-injected rats. CONCLUSION: Stimulation of PMNs by SAV leads to upregulation of MHC-II, CD 80, and CD 86, which plays critical roles in antigen presentation and consequently proliferation of T-cells. Subcutaneous route was more efficient than intraperitoneal by elevating MHC-II, CD80 and CD86 expression, disturbing oxidative stability and increasing lipogram concentration. Centro de Estudos de Venenos e Animais Peçonhentos 2019-12-02 /pmc/articles/PMC6892565/ /pubmed/31839800 http://dx.doi.org/10.1590/1678-9199-JVATITD-2019-0020 Text en This article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ebaid, Hossam
Abdel-Salam, Bahaa
Alhazza, Ibrahim
Al-Tamimi, Jameel
Hassan, Iftekhar
Rady, Ahmed
Mashaly, Ashraf
Mahmoud, Ahmed
Sammour, Reda
Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo
title Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo
title_full Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo
title_fullStr Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo
title_full_unstemmed Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo
title_short Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo
title_sort samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892565/
https://www.ncbi.nlm.nih.gov/pubmed/31839800
http://dx.doi.org/10.1590/1678-9199-JVATITD-2019-0020
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