Cargando…

Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome

Willaertia magna c2c maky is a thermophilic amoeba closely related to the genus Naegleria. This free-living amoeba has the ability to eliminate Legionella pneumophila, which is an amoeba-resisting bacterium living in an aquatic environment. To prevent the proliferation of L. pneumophila in cooling t...

Descripción completa

Detalles Bibliográficos
Autores principales: Hasni, Issam, Chelkha, Nisrine, Baptiste, Emeline, Mameri, Mouh Rayane, Lachuer, Joel, Plasson, Fabrice, Colson, Philippe, La Scola, Bernard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892926/
https://www.ncbi.nlm.nih.gov/pubmed/31797948
http://dx.doi.org/10.1038/s41598-019-54580-6
_version_ 1783476113400397824
author Hasni, Issam
Chelkha, Nisrine
Baptiste, Emeline
Mameri, Mouh Rayane
Lachuer, Joel
Plasson, Fabrice
Colson, Philippe
La Scola, Bernard
author_facet Hasni, Issam
Chelkha, Nisrine
Baptiste, Emeline
Mameri, Mouh Rayane
Lachuer, Joel
Plasson, Fabrice
Colson, Philippe
La Scola, Bernard
author_sort Hasni, Issam
collection PubMed
description Willaertia magna c2c maky is a thermophilic amoeba closely related to the genus Naegleria. This free-living amoeba has the ability to eliminate Legionella pneumophila, which is an amoeba-resisting bacterium living in an aquatic environment. To prevent the proliferation of L. pneumophila in cooling towers, the use of W. magna as natural biocide has been proposed. To provide a better understanding of the W. magna genome, whole-genome sequencing was performed through the study of virulence factors and lateral gene transfers. This amoeba harbors a genome of 36.5 megabases with 18,519 predicted genes. BLASTp analyses reported protein homology between 136 W. magna sequences and amoeba-resistant microorganisms. Horizontal gene transfers were observed based on the basis of the phylogenetic reconstruction hypothesis. We detected 15 homologs of N. fowleri genes related to virulence, although these latter were also found in the genome of N. gruberi, which is a non-pathogenic amoeba. Furthermore, the cytotoxicity test performed on human cells supports the hypothesis that the strain c2c maky is a non-pathogenic amoeba. This work explores the genomic repertory for the first draft genome of genus Willaertia and provides genomic data for further comparative studies on virulence of related pathogenic amoeba, N. fowleri.
format Online
Article
Text
id pubmed-6892926
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-68929262019-12-11 Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome Hasni, Issam Chelkha, Nisrine Baptiste, Emeline Mameri, Mouh Rayane Lachuer, Joel Plasson, Fabrice Colson, Philippe La Scola, Bernard Sci Rep Article Willaertia magna c2c maky is a thermophilic amoeba closely related to the genus Naegleria. This free-living amoeba has the ability to eliminate Legionella pneumophila, which is an amoeba-resisting bacterium living in an aquatic environment. To prevent the proliferation of L. pneumophila in cooling towers, the use of W. magna as natural biocide has been proposed. To provide a better understanding of the W. magna genome, whole-genome sequencing was performed through the study of virulence factors and lateral gene transfers. This amoeba harbors a genome of 36.5 megabases with 18,519 predicted genes. BLASTp analyses reported protein homology between 136 W. magna sequences and amoeba-resistant microorganisms. Horizontal gene transfers were observed based on the basis of the phylogenetic reconstruction hypothesis. We detected 15 homologs of N. fowleri genes related to virulence, although these latter were also found in the genome of N. gruberi, which is a non-pathogenic amoeba. Furthermore, the cytotoxicity test performed on human cells supports the hypothesis that the strain c2c maky is a non-pathogenic amoeba. This work explores the genomic repertory for the first draft genome of genus Willaertia and provides genomic data for further comparative studies on virulence of related pathogenic amoeba, N. fowleri. Nature Publishing Group UK 2019-12-04 /pmc/articles/PMC6892926/ /pubmed/31797948 http://dx.doi.org/10.1038/s41598-019-54580-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hasni, Issam
Chelkha, Nisrine
Baptiste, Emeline
Mameri, Mouh Rayane
Lachuer, Joel
Plasson, Fabrice
Colson, Philippe
La Scola, Bernard
Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
title Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
title_full Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
title_fullStr Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
title_full_unstemmed Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
title_short Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
title_sort investigation of potential pathogenicity of willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892926/
https://www.ncbi.nlm.nih.gov/pubmed/31797948
http://dx.doi.org/10.1038/s41598-019-54580-6
work_keys_str_mv AT hasniissam investigationofpotentialpathogenicityofwillaertiamagnabyinvestigatingthetransferofbacteriapathogenicitygenesintoitsgenome
AT chelkhanisrine investigationofpotentialpathogenicityofwillaertiamagnabyinvestigatingthetransferofbacteriapathogenicitygenesintoitsgenome
AT baptisteemeline investigationofpotentialpathogenicityofwillaertiamagnabyinvestigatingthetransferofbacteriapathogenicitygenesintoitsgenome
AT mamerimouhrayane investigationofpotentialpathogenicityofwillaertiamagnabyinvestigatingthetransferofbacteriapathogenicitygenesintoitsgenome
AT lachuerjoel investigationofpotentialpathogenicityofwillaertiamagnabyinvestigatingthetransferofbacteriapathogenicitygenesintoitsgenome
AT plassonfabrice investigationofpotentialpathogenicityofwillaertiamagnabyinvestigatingthetransferofbacteriapathogenicitygenesintoitsgenome
AT colsonphilippe investigationofpotentialpathogenicityofwillaertiamagnabyinvestigatingthetransferofbacteriapathogenicitygenesintoitsgenome
AT lascolabernard investigationofpotentialpathogenicityofwillaertiamagnabyinvestigatingthetransferofbacteriapathogenicitygenesintoitsgenome