Formaldehyde quantification using ampicillin is not selective

Formaldehyde (HCHO) is a simple and highly reactive human metabolite but its biochemistry is poorly defined. A limiting factor in HCHO research is lack of validated quantification methods for HCHO relevant to biological samples. We describe spectroscopic studies on a reported fluorescence-based HCHO...

Descripción completa

Detalles Bibliográficos
Autores principales: Reinbold, Raphael, John, Tobias, Spingardi, Paolo, Kawamura, Akane, Thompson, Amber L., Schofield, Christopher J., Hopkinson, Richard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892939/
https://www.ncbi.nlm.nih.gov/pubmed/31797955
http://dx.doi.org/10.1038/s41598-019-54610-3
_version_ 1783476116557660160
author Reinbold, Raphael
John, Tobias
Spingardi, Paolo
Kawamura, Akane
Thompson, Amber L.
Schofield, Christopher J.
Hopkinson, Richard J.
author_facet Reinbold, Raphael
John, Tobias
Spingardi, Paolo
Kawamura, Akane
Thompson, Amber L.
Schofield, Christopher J.
Hopkinson, Richard J.
author_sort Reinbold, Raphael
collection PubMed
description Formaldehyde (HCHO) is a simple and highly reactive human metabolite but its biochemistry is poorly defined. A limiting factor in HCHO research is lack of validated quantification methods for HCHO relevant to biological samples. We describe spectroscopic studies on a reported fluorescence-based HCHO detection method involving its reaction with ampicillin. The results validate the structure and fluorescence properties of the HCHO-ampicillin reaction product. However, the same adduct is observed after reaction of ampicillin with glyoxylate. Related fluorophores were formed with other biologically relevant carbonyl compounds. Overall, our studies suggest the ampicillin method is not reliable for selective detection and quantification of HCHO in biological samples.
format Online
Article
Text
id pubmed-6892939
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-68929392019-12-11 Formaldehyde quantification using ampicillin is not selective Reinbold, Raphael John, Tobias Spingardi, Paolo Kawamura, Akane Thompson, Amber L. Schofield, Christopher J. Hopkinson, Richard J. Sci Rep Article Formaldehyde (HCHO) is a simple and highly reactive human metabolite but its biochemistry is poorly defined. A limiting factor in HCHO research is lack of validated quantification methods for HCHO relevant to biological samples. We describe spectroscopic studies on a reported fluorescence-based HCHO detection method involving its reaction with ampicillin. The results validate the structure and fluorescence properties of the HCHO-ampicillin reaction product. However, the same adduct is observed after reaction of ampicillin with glyoxylate. Related fluorophores were formed with other biologically relevant carbonyl compounds. Overall, our studies suggest the ampicillin method is not reliable for selective detection and quantification of HCHO in biological samples. Nature Publishing Group UK 2019-12-04 /pmc/articles/PMC6892939/ /pubmed/31797955 http://dx.doi.org/10.1038/s41598-019-54610-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Reinbold, Raphael
John, Tobias
Spingardi, Paolo
Kawamura, Akane
Thompson, Amber L.
Schofield, Christopher J.
Hopkinson, Richard J.
Formaldehyde quantification using ampicillin is not selective
title Formaldehyde quantification using ampicillin is not selective
title_full Formaldehyde quantification using ampicillin is not selective
title_fullStr Formaldehyde quantification using ampicillin is not selective
title_full_unstemmed Formaldehyde quantification using ampicillin is not selective
title_short Formaldehyde quantification using ampicillin is not selective
title_sort formaldehyde quantification using ampicillin is not selective
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6892939/
https://www.ncbi.nlm.nih.gov/pubmed/31797955
http://dx.doi.org/10.1038/s41598-019-54610-3
work_keys_str_mv AT reinboldraphael formaldehydequantificationusingampicillinisnotselective
AT johntobias formaldehydequantificationusingampicillinisnotselective
AT spingardipaolo formaldehydequantificationusingampicillinisnotselective
AT kawamuraakane formaldehydequantificationusingampicillinisnotselective
AT thompsonamberl formaldehydequantificationusingampicillinisnotselective
AT schofieldchristopherj formaldehydequantificationusingampicillinisnotselective
AT hopkinsonrichardj formaldehydequantificationusingampicillinisnotselective

Ejemplares similares