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An Atlas of Transcription Factors Expressed in Male Pupal Terminalia of Drosophila melanogaster

During development, transcription factors and signaling molecules govern gene regulatory networks to direct the formation of unique morphologies. As changes in gene regulatory networks are often implicated in morphological evolution, mapping transcription factor landscapes is important, especially i...

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Autores principales: Vincent, Ben J., Rice, Gavin R., Wong, Gabriella M., Glassford, William J., Downs, Kayla I., Shastay, Jessica L., Charles-Obi, Kenechukwu, Natarajan, Malini, Gogol, Madelaine, Zeitlinger, Julia, Rebeiz, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893207/
https://www.ncbi.nlm.nih.gov/pubmed/31619460
http://dx.doi.org/10.1534/g3.119.400788
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author Vincent, Ben J.
Rice, Gavin R.
Wong, Gabriella M.
Glassford, William J.
Downs, Kayla I.
Shastay, Jessica L.
Charles-Obi, Kenechukwu
Natarajan, Malini
Gogol, Madelaine
Zeitlinger, Julia
Rebeiz, Mark
author_facet Vincent, Ben J.
Rice, Gavin R.
Wong, Gabriella M.
Glassford, William J.
Downs, Kayla I.
Shastay, Jessica L.
Charles-Obi, Kenechukwu
Natarajan, Malini
Gogol, Madelaine
Zeitlinger, Julia
Rebeiz, Mark
author_sort Vincent, Ben J.
collection PubMed
description During development, transcription factors and signaling molecules govern gene regulatory networks to direct the formation of unique morphologies. As changes in gene regulatory networks are often implicated in morphological evolution, mapping transcription factor landscapes is important, especially in tissues that undergo rapid evolutionary change. The terminalia (genital and anal structures) of Drosophila melanogaster and its close relatives exhibit dramatic changes in morphology between species. While previous studies have identified network components important for patterning the larval genital disc, the networks governing adult structures during pupal development have remained uncharted. Here, we performed RNA-seq in whole Drosophila melanogaster male terminalia followed by in situ hybridization for 100 highly expressed transcription factors during pupal development. We find that the male terminalia are highly patterned during pupal stages and that specific transcription factors mark separate structures and substructures. Our results are housed online in a searchable database (https://flyterminalia.pitt.edu/) as a resource for the community. This work lays a foundation for future investigations into the gene regulatory networks governing the development and evolution of Drosophila terminalia.
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spelling pubmed-68932072019-12-05 An Atlas of Transcription Factors Expressed in Male Pupal Terminalia of Drosophila melanogaster Vincent, Ben J. Rice, Gavin R. Wong, Gabriella M. Glassford, William J. Downs, Kayla I. Shastay, Jessica L. Charles-Obi, Kenechukwu Natarajan, Malini Gogol, Madelaine Zeitlinger, Julia Rebeiz, Mark G3 (Bethesda) Software and Data Resources During development, transcription factors and signaling molecules govern gene regulatory networks to direct the formation of unique morphologies. As changes in gene regulatory networks are often implicated in morphological evolution, mapping transcription factor landscapes is important, especially in tissues that undergo rapid evolutionary change. The terminalia (genital and anal structures) of Drosophila melanogaster and its close relatives exhibit dramatic changes in morphology between species. While previous studies have identified network components important for patterning the larval genital disc, the networks governing adult structures during pupal development have remained uncharted. Here, we performed RNA-seq in whole Drosophila melanogaster male terminalia followed by in situ hybridization for 100 highly expressed transcription factors during pupal development. We find that the male terminalia are highly patterned during pupal stages and that specific transcription factors mark separate structures and substructures. Our results are housed online in a searchable database (https://flyterminalia.pitt.edu/) as a resource for the community. This work lays a foundation for future investigations into the gene regulatory networks governing the development and evolution of Drosophila terminalia. Genetics Society of America 2019-10-16 /pmc/articles/PMC6893207/ /pubmed/31619460 http://dx.doi.org/10.1534/g3.119.400788 Text en Copyright © 2019 Vincent et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Software and Data Resources
Vincent, Ben J.
Rice, Gavin R.
Wong, Gabriella M.
Glassford, William J.
Downs, Kayla I.
Shastay, Jessica L.
Charles-Obi, Kenechukwu
Natarajan, Malini
Gogol, Madelaine
Zeitlinger, Julia
Rebeiz, Mark
An Atlas of Transcription Factors Expressed in Male Pupal Terminalia of Drosophila melanogaster
title An Atlas of Transcription Factors Expressed in Male Pupal Terminalia of Drosophila melanogaster
title_full An Atlas of Transcription Factors Expressed in Male Pupal Terminalia of Drosophila melanogaster
title_fullStr An Atlas of Transcription Factors Expressed in Male Pupal Terminalia of Drosophila melanogaster
title_full_unstemmed An Atlas of Transcription Factors Expressed in Male Pupal Terminalia of Drosophila melanogaster
title_short An Atlas of Transcription Factors Expressed in Male Pupal Terminalia of Drosophila melanogaster
title_sort atlas of transcription factors expressed in male pupal terminalia of drosophila melanogaster
topic Software and Data Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893207/
https://www.ncbi.nlm.nih.gov/pubmed/31619460
http://dx.doi.org/10.1534/g3.119.400788
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