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Silent Circulation of the Saint Louis Encephalitis Virus among Humans and Equids, Southeast Brazil
Saint Louis encephalitis virus (SLEV) is a mosquito-borne flavivirus that occurs throughout the Americas, and is considered a public health threat. In Brazil, SLEV has been detected from human cases associated with dengue-like disease, but no neurological symptoms were reported. Furthermore, the epi...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893452/ https://www.ncbi.nlm.nih.gov/pubmed/31694207 http://dx.doi.org/10.3390/v11111029 |
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author | Barbosa Costa, Galileu Marinho, Paula Eillany Silva Vilela, Ana Paula Pessoa Saraiva-Silva, Ana Teresa Crispim, Ana Paula Correia Borges, Iara Apolinário Dutra, Ana Gabriella Stoffella Lobato, Zélia Inês Portela dos Reis, Jenner Karlison Pimenta de Oliveira, Danilo Bretas Drumond, Betania Paiva Kroon, Erna Geessien Trindade, Giliane de Souza |
author_facet | Barbosa Costa, Galileu Marinho, Paula Eillany Silva Vilela, Ana Paula Pessoa Saraiva-Silva, Ana Teresa Crispim, Ana Paula Correia Borges, Iara Apolinário Dutra, Ana Gabriella Stoffella Lobato, Zélia Inês Portela dos Reis, Jenner Karlison Pimenta de Oliveira, Danilo Bretas Drumond, Betania Paiva Kroon, Erna Geessien Trindade, Giliane de Souza |
author_sort | Barbosa Costa, Galileu |
collection | PubMed |
description | Saint Louis encephalitis virus (SLEV) is a mosquito-borne flavivirus that occurs throughout the Americas, and is considered a public health threat. In Brazil, SLEV has been detected from human cases associated with dengue-like disease, but no neurological symptoms were reported. Furthermore, the epidemiology of SLEV in human populations is still poorly explored in the country. We reported serological and molecular detection of SLEV in a healthy population of equids and humans from rural areas in Southeast Brazil. A plaque reduction neutralization test was applied, and neutralizing antibodies were detected in 11 individuals (4.6%) and 60 horses (21.5%). A qPCR targeting the 5′UTR region and reverse transcription-PCR (RT-PCR) targeting the non-structural protein (NS5) gene were performed and three individuals tested positive in both assays. Subsequent phylogenetic analysis confirmed SLEV circulation and its findings suggest the occurrence of an asymptomatic or subclinical presence in human and animal cases, correlating with the risks for outbreaks and consequently burden of SLEV infections to public health. Preventive strategies should include improved surveillance in regions with a high probability of SLEV occurrence, improvement in diagnostic methods, and evaluation of exposure/risk factors that can favor SLEV emergence. |
format | Online Article Text |
id | pubmed-6893452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68934522019-12-23 Silent Circulation of the Saint Louis Encephalitis Virus among Humans and Equids, Southeast Brazil Barbosa Costa, Galileu Marinho, Paula Eillany Silva Vilela, Ana Paula Pessoa Saraiva-Silva, Ana Teresa Crispim, Ana Paula Correia Borges, Iara Apolinário Dutra, Ana Gabriella Stoffella Lobato, Zélia Inês Portela dos Reis, Jenner Karlison Pimenta de Oliveira, Danilo Bretas Drumond, Betania Paiva Kroon, Erna Geessien Trindade, Giliane de Souza Viruses Article Saint Louis encephalitis virus (SLEV) is a mosquito-borne flavivirus that occurs throughout the Americas, and is considered a public health threat. In Brazil, SLEV has been detected from human cases associated with dengue-like disease, but no neurological symptoms were reported. Furthermore, the epidemiology of SLEV in human populations is still poorly explored in the country. We reported serological and molecular detection of SLEV in a healthy population of equids and humans from rural areas in Southeast Brazil. A plaque reduction neutralization test was applied, and neutralizing antibodies were detected in 11 individuals (4.6%) and 60 horses (21.5%). A qPCR targeting the 5′UTR region and reverse transcription-PCR (RT-PCR) targeting the non-structural protein (NS5) gene were performed and three individuals tested positive in both assays. Subsequent phylogenetic analysis confirmed SLEV circulation and its findings suggest the occurrence of an asymptomatic or subclinical presence in human and animal cases, correlating with the risks for outbreaks and consequently burden of SLEV infections to public health. Preventive strategies should include improved surveillance in regions with a high probability of SLEV occurrence, improvement in diagnostic methods, and evaluation of exposure/risk factors that can favor SLEV emergence. MDPI 2019-11-05 /pmc/articles/PMC6893452/ /pubmed/31694207 http://dx.doi.org/10.3390/v11111029 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Barbosa Costa, Galileu Marinho, Paula Eillany Silva Vilela, Ana Paula Pessoa Saraiva-Silva, Ana Teresa Crispim, Ana Paula Correia Borges, Iara Apolinário Dutra, Ana Gabriella Stoffella Lobato, Zélia Inês Portela dos Reis, Jenner Karlison Pimenta de Oliveira, Danilo Bretas Drumond, Betania Paiva Kroon, Erna Geessien Trindade, Giliane de Souza Silent Circulation of the Saint Louis Encephalitis Virus among Humans and Equids, Southeast Brazil |
title | Silent Circulation of the Saint Louis Encephalitis Virus among Humans and Equids, Southeast Brazil |
title_full | Silent Circulation of the Saint Louis Encephalitis Virus among Humans and Equids, Southeast Brazil |
title_fullStr | Silent Circulation of the Saint Louis Encephalitis Virus among Humans and Equids, Southeast Brazil |
title_full_unstemmed | Silent Circulation of the Saint Louis Encephalitis Virus among Humans and Equids, Southeast Brazil |
title_short | Silent Circulation of the Saint Louis Encephalitis Virus among Humans and Equids, Southeast Brazil |
title_sort | silent circulation of the saint louis encephalitis virus among humans and equids, southeast brazil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893452/ https://www.ncbi.nlm.nih.gov/pubmed/31694207 http://dx.doi.org/10.3390/v11111029 |
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