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Impact of Vitamin D Deficit on the Rat Gut Microbiome

Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven week...

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Autores principales: Robles-Vera, Iñaki, Callejo, María, Ramos, Ricardo, Duarte, Juan, Perez-Vizcaino, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893507/
https://www.ncbi.nlm.nih.gov/pubmed/31652902
http://dx.doi.org/10.3390/nu11112564
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author Robles-Vera, Iñaki
Callejo, María
Ramos, Ricardo
Duarte, Juan
Perez-Vizcaino, Francisco
author_facet Robles-Vera, Iñaki
Callejo, María
Ramos, Ricardo
Duarte, Juan
Perez-Vizcaino, Francisco
author_sort Robles-Vera, Iñaki
collection PubMed
description Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on α- diversity but no effect on β-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis.
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spelling pubmed-68935072019-12-23 Impact of Vitamin D Deficit on the Rat Gut Microbiome Robles-Vera, Iñaki Callejo, María Ramos, Ricardo Duarte, Juan Perez-Vizcaino, Francisco Nutrients Article Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on α- diversity but no effect on β-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis. MDPI 2019-10-24 /pmc/articles/PMC6893507/ /pubmed/31652902 http://dx.doi.org/10.3390/nu11112564 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Robles-Vera, Iñaki
Callejo, María
Ramos, Ricardo
Duarte, Juan
Perez-Vizcaino, Francisco
Impact of Vitamin D Deficit on the Rat Gut Microbiome
title Impact of Vitamin D Deficit on the Rat Gut Microbiome
title_full Impact of Vitamin D Deficit on the Rat Gut Microbiome
title_fullStr Impact of Vitamin D Deficit on the Rat Gut Microbiome
title_full_unstemmed Impact of Vitamin D Deficit on the Rat Gut Microbiome
title_short Impact of Vitamin D Deficit on the Rat Gut Microbiome
title_sort impact of vitamin d deficit on the rat gut microbiome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893507/
https://www.ncbi.nlm.nih.gov/pubmed/31652902
http://dx.doi.org/10.3390/nu11112564
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