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Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet

Porcine reproductive and respiratory syndrome virus (PRRSV) is a huge threat to the modern pig industry, and current vaccine prevention strategies could not provide full protection against it. Therefore, exploring new anti-PRRSV strategies is urgently needed. Ginsenoside Rg1, derived from ginseng an...

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Autores principales: Yu, Zhi-qing, Yi, He-you, Ma, Jun, Wei, Ying-fang, Cai, Meng-kai, Li, Qi, Qin, Chen-xiao, Chen, Yong-jie, Han, Xiao-liang, Zhong, Ru-ting, Chen, Yao, Liang, Guan, Deng, Qiwei, Tian, Kegong, Wang, Heng, Zhang, Gui-hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893584/
https://www.ncbi.nlm.nih.gov/pubmed/31717616
http://dx.doi.org/10.3390/v11111045
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author Yu, Zhi-qing
Yi, He-you
Ma, Jun
Wei, Ying-fang
Cai, Meng-kai
Li, Qi
Qin, Chen-xiao
Chen, Yong-jie
Han, Xiao-liang
Zhong, Ru-ting
Chen, Yao
Liang, Guan
Deng, Qiwei
Tian, Kegong
Wang, Heng
Zhang, Gui-hong
author_facet Yu, Zhi-qing
Yi, He-you
Ma, Jun
Wei, Ying-fang
Cai, Meng-kai
Li, Qi
Qin, Chen-xiao
Chen, Yong-jie
Han, Xiao-liang
Zhong, Ru-ting
Chen, Yao
Liang, Guan
Deng, Qiwei
Tian, Kegong
Wang, Heng
Zhang, Gui-hong
author_sort Yu, Zhi-qing
collection PubMed
description Porcine reproductive and respiratory syndrome virus (PRRSV) is a huge threat to the modern pig industry, and current vaccine prevention strategies could not provide full protection against it. Therefore, exploring new anti-PRRSV strategies is urgently needed. Ginsenoside Rg1, derived from ginseng and notoginseng, is shown to exert anti-inflammatory, neuronal apoptosis-suppressing and anti-oxidant effects. Here we demonstrate Rg1-inhibited PRRSV infection both in Marc-145 cells and porcine alveolar macrophages (PAMs) in a dose-dependent manner. Rg1 treatment affected multiple steps of the PRRSV lifecycle, including virus attachment, replication and release at concentrations of 10 or 50 µM. Meanwhile, Rg1 exhibited broad inhibitory activities against Type 2 PRRSV, including highly pathogenic PRRSV (HP-PRRSV) XH-GD and JXA1, NADC-30-like strain HNLY and classical strain VR2332. Mechanistically, Rg1 reduced mRNA levels of the pro-inflammatory cytokines, including IL-1β, IL-8, IL-6 and TNF-α, and decreased NF-κB signaling activation triggered by PRRSV infection. Furthermore, 4-week old piglets intramuscularly treated with Rg1 after being challenged with the HP-PRRSV JXA1 strain display moderate lung injury, decreased viral load in serum and tissues, and an improved survival rate. Collectively, our study provides research basis and supportive clinical data for using Ginsenoside Rg1 in PRRSV therapies in swine.
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spelling pubmed-68935842019-12-23 Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet Yu, Zhi-qing Yi, He-you Ma, Jun Wei, Ying-fang Cai, Meng-kai Li, Qi Qin, Chen-xiao Chen, Yong-jie Han, Xiao-liang Zhong, Ru-ting Chen, Yao Liang, Guan Deng, Qiwei Tian, Kegong Wang, Heng Zhang, Gui-hong Viruses Article Porcine reproductive and respiratory syndrome virus (PRRSV) is a huge threat to the modern pig industry, and current vaccine prevention strategies could not provide full protection against it. Therefore, exploring new anti-PRRSV strategies is urgently needed. Ginsenoside Rg1, derived from ginseng and notoginseng, is shown to exert anti-inflammatory, neuronal apoptosis-suppressing and anti-oxidant effects. Here we demonstrate Rg1-inhibited PRRSV infection both in Marc-145 cells and porcine alveolar macrophages (PAMs) in a dose-dependent manner. Rg1 treatment affected multiple steps of the PRRSV lifecycle, including virus attachment, replication and release at concentrations of 10 or 50 µM. Meanwhile, Rg1 exhibited broad inhibitory activities against Type 2 PRRSV, including highly pathogenic PRRSV (HP-PRRSV) XH-GD and JXA1, NADC-30-like strain HNLY and classical strain VR2332. Mechanistically, Rg1 reduced mRNA levels of the pro-inflammatory cytokines, including IL-1β, IL-8, IL-6 and TNF-α, and decreased NF-κB signaling activation triggered by PRRSV infection. Furthermore, 4-week old piglets intramuscularly treated with Rg1 after being challenged with the HP-PRRSV JXA1 strain display moderate lung injury, decreased viral load in serum and tissues, and an improved survival rate. Collectively, our study provides research basis and supportive clinical data for using Ginsenoside Rg1 in PRRSV therapies in swine. MDPI 2019-11-10 /pmc/articles/PMC6893584/ /pubmed/31717616 http://dx.doi.org/10.3390/v11111045 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Zhi-qing
Yi, He-you
Ma, Jun
Wei, Ying-fang
Cai, Meng-kai
Li, Qi
Qin, Chen-xiao
Chen, Yong-jie
Han, Xiao-liang
Zhong, Ru-ting
Chen, Yao
Liang, Guan
Deng, Qiwei
Tian, Kegong
Wang, Heng
Zhang, Gui-hong
Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet
title Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet
title_full Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet
title_fullStr Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet
title_full_unstemmed Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet
title_short Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet
title_sort ginsenoside rg1 suppresses type 2 prrsv infection via nf-κb signaling pathway in vitro, and provides partial protection against hp-prrsv in piglet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893584/
https://www.ncbi.nlm.nih.gov/pubmed/31717616
http://dx.doi.org/10.3390/v11111045
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