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Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms

Astroviruses cause disease in a variety of species. Yet, little is known about the epidemiology of a majority of astroviruses including porcine astrovirus type 3 (PoAstV3), which is a putative cause of polioencephalomyelitis in swine. Accordingly, a cross-sectional study was conducted on sow farms w...

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Autores principales: Rawal, Gaurav, Ferreyra, Franco Matias, Macedo, Nubia R., Bradner, Laura K., Harmon, Karen M., Mueller, Adam, Allison, Grant, Linhares, Daniel C.L., Arruda, Bailey L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893673/
https://www.ncbi.nlm.nih.gov/pubmed/31718108
http://dx.doi.org/10.3390/v11111051
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author Rawal, Gaurav
Ferreyra, Franco Matias
Macedo, Nubia R.
Bradner, Laura K.
Harmon, Karen M.
Mueller, Adam
Allison, Grant
Linhares, Daniel C.L.
Arruda, Bailey L.
author_facet Rawal, Gaurav
Ferreyra, Franco Matias
Macedo, Nubia R.
Bradner, Laura K.
Harmon, Karen M.
Mueller, Adam
Allison, Grant
Linhares, Daniel C.L.
Arruda, Bailey L.
author_sort Rawal, Gaurav
collection PubMed
description Astroviruses cause disease in a variety of species. Yet, little is known about the epidemiology of a majority of astroviruses including porcine astrovirus type 3 (PoAstV3), which is a putative cause of polioencephalomyelitis in swine. Accordingly, a cross-sectional study was conducted on sow farms with or without reported PoAstV3-associated neurologic disease in growing pigs weaned from those farms. Additionally, a conveniently selected subset of piglets from one farm was selected for gross and histologic evaluation. The distribution of PoAstV3 in the enteric system was evaluated through in situ hybridization. PoAstV3, as detected by RT-qPCR on fecal samples, was frequently detected across sows and piglets (66–90%) on all farms (65–85%). PoAstV3 was detected subsequently at a similar detection frequency (77% vs 85%) on one farm after three months. Viral shedding, as determined by the cycle quantification value, suggests that piglets shed higher quantities of virus than adult swine. No link between gastrointestinal disease and PoAstV3 was found. However, PoAstV3 was detected by in situ in myenteric plexus neurons of piglets elucidating a possible route of spread of the virus from the gastrointestinal tract to the central nervous system. These data suggest PoAstV3 has endemic potential, is shed in the feces at greater quantities by suckling piglets when compared to sows, and infection is widespread on farms in which it is detected.
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spelling pubmed-68936732019-12-23 Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms Rawal, Gaurav Ferreyra, Franco Matias Macedo, Nubia R. Bradner, Laura K. Harmon, Karen M. Mueller, Adam Allison, Grant Linhares, Daniel C.L. Arruda, Bailey L. Viruses Article Astroviruses cause disease in a variety of species. Yet, little is known about the epidemiology of a majority of astroviruses including porcine astrovirus type 3 (PoAstV3), which is a putative cause of polioencephalomyelitis in swine. Accordingly, a cross-sectional study was conducted on sow farms with or without reported PoAstV3-associated neurologic disease in growing pigs weaned from those farms. Additionally, a conveniently selected subset of piglets from one farm was selected for gross and histologic evaluation. The distribution of PoAstV3 in the enteric system was evaluated through in situ hybridization. PoAstV3, as detected by RT-qPCR on fecal samples, was frequently detected across sows and piglets (66–90%) on all farms (65–85%). PoAstV3 was detected subsequently at a similar detection frequency (77% vs 85%) on one farm after three months. Viral shedding, as determined by the cycle quantification value, suggests that piglets shed higher quantities of virus than adult swine. No link between gastrointestinal disease and PoAstV3 was found. However, PoAstV3 was detected by in situ in myenteric plexus neurons of piglets elucidating a possible route of spread of the virus from the gastrointestinal tract to the central nervous system. These data suggest PoAstV3 has endemic potential, is shed in the feces at greater quantities by suckling piglets when compared to sows, and infection is widespread on farms in which it is detected. MDPI 2019-11-12 /pmc/articles/PMC6893673/ /pubmed/31718108 http://dx.doi.org/10.3390/v11111051 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rawal, Gaurav
Ferreyra, Franco Matias
Macedo, Nubia R.
Bradner, Laura K.
Harmon, Karen M.
Mueller, Adam
Allison, Grant
Linhares, Daniel C.L.
Arruda, Bailey L.
Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms
title Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms
title_full Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms
title_fullStr Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms
title_full_unstemmed Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms
title_short Detection and Cellular Tropism of Porcine Astrovirus Type 3 on Breeding Farms
title_sort detection and cellular tropism of porcine astrovirus type 3 on breeding farms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893673/
https://www.ncbi.nlm.nih.gov/pubmed/31718108
http://dx.doi.org/10.3390/v11111051
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