Cargando…

Effects of Nutrient Intake Timing on Post-Exercise Glycogen Accumulation and Its Related Signaling Pathways in Mouse Skeletal Muscle

We investigated the effects of nutrient intake timing on glycogen accumulation and its related signals in skeletal muscle after an exercise that did not induce large glycogen depletion. Male ICR mice ran on a treadmill at 25 m/min for 60 min under a fed condition. Mice were orally administered a sol...

Descripción completa

Detalles Bibliográficos
Autores principales: Takahashi, Yumiko, Matsunaga, Yutaka, Banjo, Mai, Takahashi, Kenya, Sato, Yosuke, Seike, Kohei, Nakano, Suguru, Hatta, Hideo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893707/
https://www.ncbi.nlm.nih.gov/pubmed/31652791
http://dx.doi.org/10.3390/nu11112555
_version_ 1783476261759221760
author Takahashi, Yumiko
Matsunaga, Yutaka
Banjo, Mai
Takahashi, Kenya
Sato, Yosuke
Seike, Kohei
Nakano, Suguru
Hatta, Hideo
author_facet Takahashi, Yumiko
Matsunaga, Yutaka
Banjo, Mai
Takahashi, Kenya
Sato, Yosuke
Seike, Kohei
Nakano, Suguru
Hatta, Hideo
author_sort Takahashi, Yumiko
collection PubMed
description We investigated the effects of nutrient intake timing on glycogen accumulation and its related signals in skeletal muscle after an exercise that did not induce large glycogen depletion. Male ICR mice ran on a treadmill at 25 m/min for 60 min under a fed condition. Mice were orally administered a solution containing 1.2 mg/g carbohydrate and 0.4 mg/g protein or water either immediately (early nutrient, EN) or 180 min (late nutrient, LN) after the exercise. Tissues were harvested at 30 min after the oral administration. No significant difference in blood glucose or plasma insulin concentrations was found between the EN and LN groups. The plantaris muscle glycogen concentration was significantly (p < 0.05) higher in the EN group—but not in the LN group—compared to the respective time-matched control group. Akt Ser(473) phosphorylation was significantly higher in the EN group than in the time-matched control group (p < 0.01), while LN had no effect. Positive main effects of time were found for the phosphorylations in Akt substrate of 160 kDa (AS160) Thr(642) (p < 0.05), 5′-AMP-activated protein kinase (AMPK) Thr(172) (p < 0.01), and acetyl-CoA carboxylase Ser(79) (p < 0.01); however, no effect of nutrient intake was found for these. We showed that delayed nutrient intake could not increase muscle glycogen after endurance exercise which did not induce large glycogen depletion. The results also suggest that post-exercise muscle glycogen accumulation after nutrient intake might be partly influenced by Akt activation. Meanwhile, increased AS160 and AMPK activation by post-exercise fasting might not lead to glycogen accumulation.
format Online
Article
Text
id pubmed-6893707
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68937072019-12-23 Effects of Nutrient Intake Timing on Post-Exercise Glycogen Accumulation and Its Related Signaling Pathways in Mouse Skeletal Muscle Takahashi, Yumiko Matsunaga, Yutaka Banjo, Mai Takahashi, Kenya Sato, Yosuke Seike, Kohei Nakano, Suguru Hatta, Hideo Nutrients Article We investigated the effects of nutrient intake timing on glycogen accumulation and its related signals in skeletal muscle after an exercise that did not induce large glycogen depletion. Male ICR mice ran on a treadmill at 25 m/min for 60 min under a fed condition. Mice were orally administered a solution containing 1.2 mg/g carbohydrate and 0.4 mg/g protein or water either immediately (early nutrient, EN) or 180 min (late nutrient, LN) after the exercise. Tissues were harvested at 30 min after the oral administration. No significant difference in blood glucose or plasma insulin concentrations was found between the EN and LN groups. The plantaris muscle glycogen concentration was significantly (p < 0.05) higher in the EN group—but not in the LN group—compared to the respective time-matched control group. Akt Ser(473) phosphorylation was significantly higher in the EN group than in the time-matched control group (p < 0.01), while LN had no effect. Positive main effects of time were found for the phosphorylations in Akt substrate of 160 kDa (AS160) Thr(642) (p < 0.05), 5′-AMP-activated protein kinase (AMPK) Thr(172) (p < 0.01), and acetyl-CoA carboxylase Ser(79) (p < 0.01); however, no effect of nutrient intake was found for these. We showed that delayed nutrient intake could not increase muscle glycogen after endurance exercise which did not induce large glycogen depletion. The results also suggest that post-exercise muscle glycogen accumulation after nutrient intake might be partly influenced by Akt activation. Meanwhile, increased AS160 and AMPK activation by post-exercise fasting might not lead to glycogen accumulation. MDPI 2019-10-23 /pmc/articles/PMC6893707/ /pubmed/31652791 http://dx.doi.org/10.3390/nu11112555 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Takahashi, Yumiko
Matsunaga, Yutaka
Banjo, Mai
Takahashi, Kenya
Sato, Yosuke
Seike, Kohei
Nakano, Suguru
Hatta, Hideo
Effects of Nutrient Intake Timing on Post-Exercise Glycogen Accumulation and Its Related Signaling Pathways in Mouse Skeletal Muscle
title Effects of Nutrient Intake Timing on Post-Exercise Glycogen Accumulation and Its Related Signaling Pathways in Mouse Skeletal Muscle
title_full Effects of Nutrient Intake Timing on Post-Exercise Glycogen Accumulation and Its Related Signaling Pathways in Mouse Skeletal Muscle
title_fullStr Effects of Nutrient Intake Timing on Post-Exercise Glycogen Accumulation and Its Related Signaling Pathways in Mouse Skeletal Muscle
title_full_unstemmed Effects of Nutrient Intake Timing on Post-Exercise Glycogen Accumulation and Its Related Signaling Pathways in Mouse Skeletal Muscle
title_short Effects of Nutrient Intake Timing on Post-Exercise Glycogen Accumulation and Its Related Signaling Pathways in Mouse Skeletal Muscle
title_sort effects of nutrient intake timing on post-exercise glycogen accumulation and its related signaling pathways in mouse skeletal muscle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893707/
https://www.ncbi.nlm.nih.gov/pubmed/31652791
http://dx.doi.org/10.3390/nu11112555
work_keys_str_mv AT takahashiyumiko effectsofnutrientintaketimingonpostexerciseglycogenaccumulationanditsrelatedsignalingpathwaysinmouseskeletalmuscle
AT matsunagayutaka effectsofnutrientintaketimingonpostexerciseglycogenaccumulationanditsrelatedsignalingpathwaysinmouseskeletalmuscle
AT banjomai effectsofnutrientintaketimingonpostexerciseglycogenaccumulationanditsrelatedsignalingpathwaysinmouseskeletalmuscle
AT takahashikenya effectsofnutrientintaketimingonpostexerciseglycogenaccumulationanditsrelatedsignalingpathwaysinmouseskeletalmuscle
AT satoyosuke effectsofnutrientintaketimingonpostexerciseglycogenaccumulationanditsrelatedsignalingpathwaysinmouseskeletalmuscle
AT seikekohei effectsofnutrientintaketimingonpostexerciseglycogenaccumulationanditsrelatedsignalingpathwaysinmouseskeletalmuscle
AT nakanosuguru effectsofnutrientintaketimingonpostexerciseglycogenaccumulationanditsrelatedsignalingpathwaysinmouseskeletalmuscle
AT hattahideo effectsofnutrientintaketimingonpostexerciseglycogenaccumulationanditsrelatedsignalingpathwaysinmouseskeletalmuscle