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A Calorie-Restricted Ketogenic Diet Reduces Cerebral Cortex Vascularization in Prepubertal Rats

The antiepileptic effect of ketogenic diets is acknowledged but its mechanism of action is poorly understood. The present work aimed to evaluate possible effects of a calorie-restricted ketogenic diet (CRKD) on brain growth and angiogenesis in normal prepubertal rats. Two groups of prepubertal rats...

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Autores principales: Viggiano, Andrea, Meccariello, Rosaria, Santoro, Antonietta, Secondulfo, Carmine, Operto, Francesca Felicia, Monda, Marcellino, Coppola, Giangennaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893715/
https://www.ncbi.nlm.nih.gov/pubmed/31694345
http://dx.doi.org/10.3390/nu11112681
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author Viggiano, Andrea
Meccariello, Rosaria
Santoro, Antonietta
Secondulfo, Carmine
Operto, Francesca Felicia
Monda, Marcellino
Coppola, Giangennaro
author_facet Viggiano, Andrea
Meccariello, Rosaria
Santoro, Antonietta
Secondulfo, Carmine
Operto, Francesca Felicia
Monda, Marcellino
Coppola, Giangennaro
author_sort Viggiano, Andrea
collection PubMed
description The antiepileptic effect of ketogenic diets is acknowledged but its mechanism of action is poorly understood. The present work aimed to evaluate possible effects of a calorie-restricted ketogenic diet (CRKD) on brain growth and angiogenesis in normal prepubertal rats. Two groups of prepubertal rats were fed with a standard diet (group 1) or a CRKD (group 2) for ten weeks. Then, rats were sacrificed and the thickness for the following structures was evaluated by histology: (1) cerebral cortex, (2) deep cerebral white matter, and (3) substantia nigra. The capillary density was also evaluated within: (1) cerebral cortex, (2) dentate gyrus of the hippocampus, (3) periaqueductal grey matter, and (4) substantia nigra. The results showed a smaller thickness of all the areas examined and a reduced capillary density within the cerebral cortex in the CRKD-treated group compared to the control group. These findings suggest an association between reduced angiogenesis within the cerebral cortex and the antiepileptic effects of CRKD.
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spelling pubmed-68937152019-12-23 A Calorie-Restricted Ketogenic Diet Reduces Cerebral Cortex Vascularization in Prepubertal Rats Viggiano, Andrea Meccariello, Rosaria Santoro, Antonietta Secondulfo, Carmine Operto, Francesca Felicia Monda, Marcellino Coppola, Giangennaro Nutrients Article The antiepileptic effect of ketogenic diets is acknowledged but its mechanism of action is poorly understood. The present work aimed to evaluate possible effects of a calorie-restricted ketogenic diet (CRKD) on brain growth and angiogenesis in normal prepubertal rats. Two groups of prepubertal rats were fed with a standard diet (group 1) or a CRKD (group 2) for ten weeks. Then, rats were sacrificed and the thickness for the following structures was evaluated by histology: (1) cerebral cortex, (2) deep cerebral white matter, and (3) substantia nigra. The capillary density was also evaluated within: (1) cerebral cortex, (2) dentate gyrus of the hippocampus, (3) periaqueductal grey matter, and (4) substantia nigra. The results showed a smaller thickness of all the areas examined and a reduced capillary density within the cerebral cortex in the CRKD-treated group compared to the control group. These findings suggest an association between reduced angiogenesis within the cerebral cortex and the antiepileptic effects of CRKD. MDPI 2019-11-05 /pmc/articles/PMC6893715/ /pubmed/31694345 http://dx.doi.org/10.3390/nu11112681 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Viggiano, Andrea
Meccariello, Rosaria
Santoro, Antonietta
Secondulfo, Carmine
Operto, Francesca Felicia
Monda, Marcellino
Coppola, Giangennaro
A Calorie-Restricted Ketogenic Diet Reduces Cerebral Cortex Vascularization in Prepubertal Rats
title A Calorie-Restricted Ketogenic Diet Reduces Cerebral Cortex Vascularization in Prepubertal Rats
title_full A Calorie-Restricted Ketogenic Diet Reduces Cerebral Cortex Vascularization in Prepubertal Rats
title_fullStr A Calorie-Restricted Ketogenic Diet Reduces Cerebral Cortex Vascularization in Prepubertal Rats
title_full_unstemmed A Calorie-Restricted Ketogenic Diet Reduces Cerebral Cortex Vascularization in Prepubertal Rats
title_short A Calorie-Restricted Ketogenic Diet Reduces Cerebral Cortex Vascularization in Prepubertal Rats
title_sort calorie-restricted ketogenic diet reduces cerebral cortex vascularization in prepubertal rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893715/
https://www.ncbi.nlm.nih.gov/pubmed/31694345
http://dx.doi.org/10.3390/nu11112681
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